Abstract
Highly purified hen oviduct progesterone receptor A subunit has been found to interact preferentially with the 5’-flanking region of the chicken ovalbumin gene. Saturation kinetics showed that 2-fold less receptor was required for half-maximal binding to ovalbumin gene flanking sequences than to plasmid pBR322 sequences. Studies of selective binding were facilitated by a preferential adsorption assay involving gel electrophoretic analysis of [32P]DNA restriction fragments eluted from receptor-DNA complexes isolated on nitrocellulose filters. Approximately 10-fold difference in binding was observed at 37°C for a 1.7 Kb ovalbumin DNA insert compared to binding of receptor to a 3.6 Kb plasmid pBR322 fragment. Selective receptor A binding was dramatically reduced when assayed at 4°C. In addition to temperature dependence, preferential binding required the presence of glycerol or dimethyl sulfoxide during binding. Divalent cations and ionic strength had no apparent effect on the relative sequence selectivity but did alter the total DNA binding observed. Binding data from a variety of different restriction digests suggest receptor A interacts between −135 and −249 bp (upstream) from the start of ovalbumin transcription. Preferential binding to a DNA fragment containing the sequence −16 to −247 compared to an adjacent upstream fragment or plasmid DNA fragment confirm this assignment. This binding site lies within the regulatory sequence required for progesterone induction of a transfected fusion gene in primary chicken oviduct cells.
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References
B. W. O’Malley, D. R. Roop, E. C. Lai, J. L. Nordstrom, J. F. Catterall, G. E. Swaneck, D. A. Colbert, M. J. Tsai, A. Dugaiczyk, S. L. C. Woo, The ovalbumin gene: organization, structure, transcription and regulation. Recent Prog. Horm. Res. 35: 1 (1979).
M. LeMeur, N. Glanville, J. L. Mandel, P. Gerlinger, R. Palmiter, P. Chambon, The ovalbumin gene family: Hormonal control of X and Y gene transcription and mRNA accumulation. Cell 23: 561 (1981).
G. E. Swaneck, J. L. Nordstrom, F. Kruezaler, M. J. Tsai, O’Malley, The effect of estrogen on gene expression in the chicken oviduct: Evidence for transcriptional control of ovalbumin gene. Proc. Natl. Acad. Sci. USA 76: 1049 (1979).
G. S. McKnight, R. D. Palmiter, Transcriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct. J. Biol. Chem. 254: 9050 (1979).
J. Mester, E. E. Beaulieu, Progesterone receptors in chick oviduct. Determination of total concentration of binding sites in cytosol and nuclear fraction and effect of progesterone on their distribution. Eur. J. Biochem. 72: 405 (1977).
W. T. Schrader, B. W. O’Malley, Progesterone-binding components of chick oviduct. IV. Characterization of purified subunits. J. Biol. Chem. 247: 51 (1972).
P. E. Hansen, A. Johnson, W. T. Schrader, B. W. O’Malley, Kinetics of progesterone binding to the chick oviduct receptor protein. J. Steroid Biochem. 7: 723 (1974).
W. V. Vedeckis, W. T. Schrader, B. W. O’Malley, The chick oviduct progesterone receptor. Biochem. Actions Horm. 5: 321 (1978).
M. R. Hughes, N. L. Weigel, W. W. Grody, B. W. O’Malley, Studies on the structure and function of the chicken progesterone receptor. Recent Prog. Horm. Res. 37: 51 (1981).
N. L. Weigel, P. P. Minghetti, B. Stevens, W. T. Schrader, B. W. O’Malley, Identification of the structure of the chicken progesterone receptor. In: Steroid Hormone Receptors: Structure and Function, Eriksson H, and Gustafsson JA, (eds), Elsevier, Amsterdam p 25 (1983).
W. A. Coty, W. T. Schrader, B. W. O’Malley, Purification and characterization of the chicken oviduct progesterone receptor A subunit. J. Steroid Biochem. 10: 1 (1979).
N. L. Weigel, J. S. Tash, A. R. Means, W. T. Schrader and B. W. O’Malley, Phosphorylation of hen progesterone receptor by cAMP dependent protein kinase. Biochem. Biophys. Res. Commun. 102: 513 (1981).
W. T. Schrader, D. 0. Toft, B. W. O’Malley, Progesterone-binding protein of chick oviducts. VI. Interaction of purified progesterone-receptor components with nuclear constituents. J. Biol. Chem. 247: 2401 (1972).
E. R. Mulvihill, J. P. LePennec, P. Chambon, Chicken oviduct progesterone receptors: Location of specific regions of high affinity binding in cloned DNA fragments of hormone responsive genes. Cell 24: 621 (1982).
J. G. Compton, W. T. Schrader, B. W. O’Malley, Progesterone receptor binding to DNA: Studies by sedimentation velocity methods. J. Steroid Biochem. 20: 89 (1984).
A. D. Riggs, H. Suzuki, S. Bouregeois, Lac repressor-operator interaction. 1. Equilibrium studies. J. Mol. Biol. 48: 67 (1970).
M. R. Hughes, J. G. Compton, W. T. Schrader, B. W. O’Malley, Interaction of the chick oviduct progesterone receptor with deoxyribonucleic acid. Biochemistry 20: 2481 (1981).
J. G. Compton, W. T. Schrader, B. W. O’Malley, Selective binding of chicken progesterone receptor A subunit to a DNA fragment containing ovalbumin gene sequences. Biochem. Biophys. Res. Commun. 104: 96 (1982).
J. G. Compton, W. T. Schrader, B. W. O’Malley, DNA sequence preference of the progesterone receptor. Proc. Natl. Acad. Sci. USA 80: 16 (1983).
D. C. Dean, B. J. Knoll, M. E. Riser, B. W. O’Malley, A 5’-flanking sequence (-95 to -222) is essential for progesterone regulation of an ovalbumin fusion gene. Nature 305: 551 (1983).
U. K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 118: 197 (1970).
A. Dugaiczyk, S. L. C. Woo, D. A. Colbert, E. C. Lai, M. L. Mace, B. W. O’Malley, The ovalbumin gene: Cloning and molecular organization of the entire natural gene. Proc. Natl. Acad. Sci. USA 76: 2253 (1979).
C. Scheidereit, S. Geisse, H. M. Westphal, N. M. Beato, The glucocorticoid receptor binds to defined nucleotide sequences near the promoter of mouse mammary tumor virus. Nature 304: 749 (1984).
M. Pfahl, D. McGinnis, M. Henricks, B. Groner, N. Hynes, Correlation of glucocorticoid receptor binding sites on MMTV proviral DNA with hormone inducible transcription. Science 222: 1341 (1984).
M. Karin, A. Haslinger, H. Holtgreve, R. I. Richards, P. Krauter, H. M. Westphal, M. Beato, Characterization of DNA sequences through which cadmium and glucocortioid hormone induce human metallothionein-IIa gene. Nature 308: 513 (1984).
B. J. Knoll, T. Zarucki-Schulz, D. C. Dean and O’Malley, Definition of the ovalbumin gene promoter by transfer of an ovalglobin fusion gene into cultured cells. Nucl. Acid Res. 11: 6733 (1983).
E. H. Davidson, H. T. Jacobs, H. J. Britten, Very short repeats and coordinate induction of genes. Nature 301: 468 (1983).
B. W. O’Malley, M. J. Tsai, W. T. Schrader, Structural considerations for the action of steroid hormones in eucaryotic cells. In: Steroid Hormone Receptors: Structure and Function, Eriksson H and Gustafsson JA (eds) Elsevier, Amsterdam p 307 (1983).
D. C. Hinkle, M. J. Chamberlain, Studies of the binding of E. coli RNA polymerase to DNA. I. The role of sigma subunit in site selection. J. Mol. Biol. 70: 157 (1972).
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© 1986 Plenum Press, New York
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Compton, J.G., Schrader, W.T., O’Malley, B.W. (1986). Progesterone Receptor Interaction in the 5’-Flanking Regulatory Region of the Ovalbumin Gene. In: Chrousos, G.P., Loriaux, D.L., Lipsett, M.B. (eds) Steroid Hormone Resistance. Advances in Experimental Medicine and Biology, vol 196. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5101-6_19
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DOI: https://doi.org/10.1007/978-1-4684-5101-6_19
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