Abstract
In much the same way that the double helix model of DNA has provided the backbone for molecular genetics, the chemiosmotic hypothesis formulated and refined by Peter Mitchell during the 1960s (Mitchell, 1961, 1963, 1966) is now the conceptual framework for a wide variety of bioenergetic phenomena. In its most general form (Fig. 1), the chemiosmotic concept postulates that the immediate driving force for many processes in energy-coupling membranes is a H+ electrochemical gradient (\(Delta \overline \mu _{H^ + }\) ) composed of electrical and chemical parameters according to the following relationship: \( Delta \overline \mu _{H^ + } /F = \Delta \psi - 2.3RT/F \Delta pH \) where Δψ represents the electrical potential across the membrane and the ΔpH is the chemical difference in H+ concentration across the membrane (R is the gas constant, T is absolute temperature, F is the Faraday constant; 2.3RT/F is equal to 58.8 at room temperature) (Mitchell, 1961).
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References
Beyreuther, K., Beiseler, B., Ehring, R., and Müller-Hill, B., 1981, Identification of internal residues of lactose permease of Escherichia coli by radiolabel of peptide mixtures, in: Methods in Protein Sequence Analysis (M. Elzina, ed.), Humana Press, Clifton, NJ, p. 139.
Carrasco, N., Herzlinger, D., DeChiara, S., Danho, W., Gabriel, T. F., and Kaback, H. R., 1983, Topology of the lac protein in the membrane of Escherichia coli, Biophys. J. 45:83a.
Carrasco, N., Herzlinger, D., Mitchell, R., DeChiara, S., Danho, W., Gabriel, T. F., and Kaback, H. R., 1984a, Intramolecular dislocation of the COOH-terminus of the lac carrier protein in reconstituted proteoliposomes, Proc. Natl. Acad. Sci. U.S.A. 81:4672–4676.
Carrasco, N., Viitanen, P., Herzlinger, D., and Kaback, H. R., 1984b, Monoclonal antibodies against the lac carrier protein from Escherichia coli, I. Functional studies, Biochemistry 23:3681–3687.
Costello, M. J., Viitanen, P., Carrasco, N., Foster, D. L., and Kaback, H. R., 1984, Morphology of proteoliposomes reconstituted with purified lac carrier protein from Escherichia coli, J. Biol. Chem. 259:15579–15586.
Foster, D. L., Boublik, M., and Kaback, H. R., 1983, Structure of the lac carrier protein of Escherichia coli, J. Biol. Chem. 258:31.
Fox, C. F., and Kennedy, E. P., 1965, Specific labeling and partial purification of the M protein, a component of the ß-galactoside transport system of Escherichia coli, Proc. Natl. Acad. Sci. U.S.A. 54:891–899.
Garcia, M. L., Viitanen, P., Foster, D. L., and Kaback, H. R., 1983, Mechanism of lactose translocation in proteoliposomes reconstituted with lac carrier protein purified from Escherichia coli. I. Effect of pH and imposed membrane potential on efflux, exchange and counterflow, Biochemistry 22:2524–2531.
Goldkorn, T., Rimon, G., and Kaback, H. R., 1983, Topology of the lac carrier protein in the membrane of Escherichia coli, Proc. Natl. Acad. Sci. U.S.A. 80:3322–3326.
Herzlinger, D., Viitanen, P., Carrasco, N., and Kaback, H. R., 1984, Monoclonal antibodies against the lac carrier protein from Escherichia coli. II. Binding studies with membrane vesicles and proteoliposomes reconstituted with purified lac carrier protein, Biochemistry 23:3688–3693.
Herzlinger, D., Carrasco, N., and Kaback, H. R., 1985, Functional and immunochemical characterization of a mutant of Escherichia coli energy uncoupled for lactose transport. Biochemistry 24:221–229.
Hong, J.-S., 1977, An ecf mutation in Escherichia coli pleiotropically affecting energy coupling in active transport but not generation or maintenance of membrane potential, J. Biol. Chem. 252:8582–8588.
Kaback, H. R., 1976, Molecular biology and energetics of membrane transport, J. Cell. Physiol. 89:575–594.
Kaback, H. R., 1983, Thelac carrier protein in Escherichia coli: From membrane to molecule, J. Membr. Biol. 76:95.
Kaback, H. R., 1986, Active transport in Escherichia coli: From membrane to molecule, in: Physiology of Membrane Disorders (T. E. Andreoli, J. F. Hoffman, D. D. Fanestil, and S. G. Schultz, eds.). Plenum Press, New York, pp. 387–407.
Matsushita, K., Patel, L., Gennis, R. B., and Kaback, H. R., 1983, Reconstitution of active transport in proteoliposomes containing cytochome o oxidase and lac carrier protein purified from Escherichia coli, Proc. Natl. Acad. Sci. U.S.A. 80:4889–4893.
Matsushita, K., Patel, L., and Kaback, H. R., 1984, Cytochromeo oxidase from Escherichia coli. Characterization of the enzyme and mechanism of electrochemical proton gradient generation, Biochemistry 23:4703–4714.
Mieschendahl, M., Büchel, D., Bocklage, H., and Müller-Hill, B., 1981, Mutations in the lac y gene of Escherichia coli define functional organization of lactose permease, Proc. Natl. Acad. Sci. U.S.A. 78:7652–7656.
Mitchell, P., 1961, Coupling of phosphorylation to electron hydrogen transfer by a chemi-osmotic type of mechanism. Nature 191:144–148’169.
Mitchell, P., 1963, Molecule, group, and electron translocation through natural membranes, Biochem. Soc. Symp. 22:142–148.
Mitchell, P., 1966, Chemiosmotic Coupling and Energy Transduction, Glynn Research Ltd., Bodmin, UK.
Overath, P., and Wright, J. K., 1983, Lactose permease: A carrier on the move, Trends Biochem. Sci. 8:404–408.
Plate, C. A., and Suit, J. L., 1981, Theeup genetic locus of Escherichia coli and its role in H+/solute symport, J. Biol. Chem. 256:12974–12980.
Seckler, R., and Wright, J. K., 1984, Sidedness of native membrane vesicles of Escherichia coli and orientation of the reconstituted lactose:H+ carrier, Eur. J. Biochem. 142:269–279.
Seckler, R., Wright, J. K., and Overath, P., 1983, Peptide-specific antibody locates the COOH terminus of the lactose carrier of Escherichia coli on the cytoplasmic side of the plasma membrane, J. Biol. Chem. 258:10817–10820.
Trumble, W. R., Viitanen, P. V., Sarkar, H. K., Poonian, M. S., and Kaback, H. R., 1984, Site-directed mutagenesis of Cys 148 in the lac carrier protein of Escherichia coli, Biochem. Biophys. Res. Commun. 119:860–867.
Viitanen, P., Garcia, M. L., Foster, D. L., Kaczorowski, G. J., and Kaback, H. R., 1983, Mechanism of lactose translocation in proteoliposomes reconstituted with lac carrier protein purified from Escherichia coli. 2. Deuterium solvent isotope effects, Biochemistry 22:2531–2536.
Viitanen, P., Garcia, M. L., and Kaback, H. R., 1984, Purified, reconstituted lac carrier protein from Escherichia coli is fully functional, Proc. Natl. Acad. Sci. U.S.A. 81:1629–1633.
Wong, P. T. S., Kashket, E. R., and Wilson, T. H., 1970, Energy coupling in the lactose transport system of Escherichia coli, Proc. Natl. Acad. Sci. U.S.A. 65:63–69.
Wright, J. K., Weigel, U., Lustig, A., Bocklage, H., Mieschendahl, M., Müller-Hill, B., and Overath, P., 1983, Does the lactose carrier of Escherichia coli function as a monomer? FEBS Lett. 162:11–15.
Zoller, M. J., and Smith, M., 1983, Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors, Methods Enzymol. 100:468–500.
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© 1986 Plenum Press, New York
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Kaback, H.R. (1986). Studies of a Biological Energy Transducer. In: Poste, G., Crooke, S.T. (eds) New Insights into Cell and Membrane Transport Processes. New Horizons in Therapeutics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5062-0_2
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DOI: https://doi.org/10.1007/978-1-4684-5062-0_2
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