Nonseparation Enzyme Immunoassays for Macromolecules

  • Ian Gibbons


Two classes of homogeneous enzyme immunoassays for macromolecules have been developed at the Syva Research Institute. The first class uses β-galactosidase together with a macromolecular substrate (Gibbons et al., 1980). The second is based on the phenomenon of “enzyme channeling” (Litman et al., 1980) involving two or more enzymes. This chapter will discuss the principles, characteristics, performance and clinical utility of both types of assay.


Serum Ferritin Label Antibody Large Bead Homogeneous Enzyme Macromolecular Substrate 
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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Ian Gibbons
    • 1
  1. 1.Syva Research InstitutePalo AltoUSA

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