Prosthetic Group Labeled Enzyme Immunoassay
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Prosthetic group labeled enzyme immunoassay (PGLEIA) is a separation-free (homogeneous) enzyme mediated immunoassay. The assay depends on: (1) the ability of a stable covalent ligand-prosthetic group conjugate to activate an apoenzyme which by itself is enzymatically inactive; and (2) the ability of anti-ligand antibody to bind ligand-prosthetic group conjugate such that the bound ligand-prosthetic group is incapable of activating the apoenzyme.
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