Prosthetic Group Labeled Enzyme Immunoassay

  • T. T. Ngo


Prosthetic group labeled enzyme immunoassay (PGLEIA) is a separation-free (homogeneous) enzyme mediated immunoassay. The assay depends on: (1) the ability of a stable covalent ligand-prosthetic group conjugate to activate an apoenzyme which by itself is enzymatically inactive; and (2) the ability of anti-ligand antibody to bind ligand-prosthetic group conjugate such that the bound ligand-prosthetic group is incapable of activating the apoenzyme.


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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • T. T. Ngo
    • 1
  1. 1.Department of Developmental and Cell BiologyUniversity of CaliforniaIrvineUSA

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