Advertisement

Prosthetic Group Labeled Enzyme Immunoassay

  • T. T. Ngo
Chapter

Abstract

Prosthetic group labeled enzyme immunoassay (PGLEIA) is a separation-free (homogeneous) enzyme mediated immunoassay. The assay depends on: (1) the ability of a stable covalent ligand-prosthetic group conjugate to activate an apoenzyme which by itself is enzymatically inactive; and (2) the ability of anti-ligand antibody to bind ligand-prosthetic group conjugate such that the bound ligand-prosthetic group is incapable of activating the apoenzyme.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Barham, D. and P. Trinder, 1972. An improved color reagent for the detrmination of blood glucose by the oxidase system, Analyst, 97, 142–145.PubMedCrossRefGoogle Scholar
  2. Cook, C.E., M.E. Twine, M. Myers, E. Amerson, J.A. Kepler, and G.F. Taylor, 1976. Theophylline radioimmunoassay: synthesis of antigen and characterization of antiserum. Res. Cm. Chem. Pathol. Pharmacol. 13, 497–504.Google Scholar
  3. Friedman, H.C., 1963. Flavin-adenine dinucleotide. In: Methods of Enzymatic Analysis, H.U. Bergmeyer, ed., Verlag Chemie, Weinheim, Vol. 4, pp. 2182–2185.Google Scholar
  4. Geoghegan, W.D., M.F. Struve, and R.E. Jordan, 1983. Adaptation of Ngo-Lenhoff peroxidase assay for solid phase ELISA. J. Immunol. Methods 60, 61–68.PubMedCrossRefGoogle Scholar
  5. Hellis, P.F., 1982. The photophysical and photochemical properties of flavins (isoalloxazines). Chem. Soc. Rev. 11, 15–39.CrossRefGoogle Scholar
  6. Massey, V. and L.D. Mendelsohn, 1979. Immobilized glucose oxidase and D-amino acid oxidase: a convenient method for the purification of flavin adenine dinucleotide and its analogs. Anal. Biochem. 95, 156–159.PubMedCrossRefGoogle Scholar
  7. Morris, D.L., R.J. Carrico, P.B. Ellis, W.E. Hornby, H.R. Schroeder, T.T. Ngo and R.C. Boguslaski, 1980, Colorimetric immunoassays with flavin adenine dinucleotide as label, in: “Innovative approaches to clinical analytical chemistry,” Lab Professional Series, American Chemical Society.Google Scholar
  8. Morris, D.L., P.B. Ellis, R.J. Carrico, F.M. Yeager, H.R. Schroeder, J.P. Albarella, R.C. Boguslaski, W.E. Hornby, and D. Rawson, 1981. Flavin adenine dinucleotide as a label in homogeneous colorimetric immunoassays. Anal. Chem. 53, 658–665.PubMedCrossRefGoogle Scholar
  9. Ngo, T.T. et al. (1981). Reference 22 in Anal. Chem. 53, 1981, 658–665 and reference 12 in Applied Biochem. Biotech. 7, 1982, 401–414.Google Scholar
  10. Ngo, T.T. and H.M. Lenhoff, 1980a, Amperometric determination of picomolar levels of flavin adenine dinucleotide by cyclic oxidation-reduction in apo-glucose oxidase system. Anal. Letters 13, 1157–1165.CrossRefGoogle Scholar
  11. Ngo, T.T. and H.M. Lenhoff, 1980, A sensitive and versatile chromogenic assay for peroxidase and peroxidase-coupled reactions. Anal. Biochem. 105, 389–397.PubMedCrossRefGoogle Scholar
  12. Ngo, T.T. and H.M. Lenhoff, 1981, Recent advances in homogeneous and separation-free enzyme immunoassays. Applied Biochem. Biotechnol. 6, 53–64.CrossRefGoogle Scholar
  13. Ngo, T.T. and H.M. Lenhoff, 1982, Enzymes as versatile labels and signal amplifiers for monitoring immunochemical reactions. Mol. Cell. Biochem. 44, 3–12.PubMedCrossRefGoogle Scholar
  14. Okuda, J., J. Nagaraine and K. Yagi, 1979, Exchange of free and bound coenzyme of flavin enzymes studied with [14C]FAD. Biochim. Biophys. Acta 566, 245–252.PubMedGoogle Scholar
  15. Solomon, B. and Y. Levin, 1976, Flavin-protein interaction in bound glucose oxidase. J. Solid-Phase Biochem. 1, 159–171.Google Scholar
  16. Tyhach, R.J., P.A. Rupchock, J.H. Pendergrass, A.C. Skjold, P.J. Smith, R.D. Johnson, J.P. Albarella, and J.A. Profitt, 1981, Adaptation of prosthetic-group-label homogeneous immunoassay to reagent-strip format. Clin. Chem. 27 (1981), 1499–1504.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • T. T. Ngo
    • 1
  1. 1.Department of Developmental and Cell BiologyUniversity of CaliforniaIrvineUSA

Personalised recommendations