Monoclonal Antibodies in Immunoenzymetric Assays

  • Stanley Y. Shimizu
  • David S. Kabakoff
  • E. Dale Sevier


A new world of immunodiagnostics emerged when Köhler and Milstein (1975) elevated antibodies to the status of “reagent grade chemicals” by producing hybrid cells capable of virtually unlimited synthesis of homogeneous antibody molecules. These monoclonal antibodies have rapidly found their niche in various versions of the immunometric — or labeled antibody — assay technique. Six years after the initial work of Köhler and Milstein (1975), routine monoclonal antibody-based immunoradiometric and immunoenzymetric assays were developed (Sevier et al., 1981). The favorable reaction conditions of these excess antibody systems (Ekins, 1981) facilitate rapid approach to equilibrium and determination of extremely low minimal detectable antigen concentrations.


Human Chorionic Gonadotropin Antigenic Determinant Polyclonal Antiserum Label Antibody Prostatic Acid Phosphatase 
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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Stanley Y. Shimizu
    • 1
  • David S. Kabakoff
    • 1
  • E. Dale Sevier
    • 1
  1. 1.Hybritech IncorporatedSan DiegoUSA

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