Radial Partition Enzyme Immunoassay
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Radial Partition Immunoassay permits highly sensitive measurements of both low and high molecular weight ligands. The entire immunoassay is conducted on a small section of filter paper and assays can be done in less than 10 minutes. A variety of labels can be used with the technology including enzymes, fluorophores, chromophores or radioisotopes. We have achieved a high degree of sensitivity using enzyme labels and fluorescent readout of enzyme activity. The general principles of the radial partition enzyme immunoassay system are illustrated in Fig. 1.
KeywordsSandwich Assay Competitive Assay Sequential Assay Glass Fiber Filter Paper Hook Effect
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