Steric Hindrance Enzyme Immunoassay (Sheia)

  • Albert Castro
  • Nobuo Monji


We have developed a new enzyme immunoassay for the quantitation of small antigens. The assay is called SHEIA (Steric Hindrance Enzyme Immunoassay) since, this assay involves the interference of complex formation between the antigen (or hapten) coupled enzyme and the enzyme inhibitor attached to agarose by antibody to the antigen (or hapten), probably due to steric hindrance (Monji and Castro, 1979). The assay principle is shown in Figure 1.


Affinity Chromatography Separation Step Normal Rabbit Serum Detectable Enzyme Activity Alpha Omega 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Castro, A., I. Priesto, C. Wunsch, G. Ertingshausen and H. Malkus (1976). Automated radioimmunoassay of choriomammotropin (human placeatal lactogen). Clin. Chem. 22: 1655–1658.PubMedGoogle Scholar
  2. Collins, W. P., G. J. R. Barnard and J. F. Hennan (1975). Factors affecting the choice of separation techniques, in Steriod Immunoassay, Proceeding of the Fifth Tenivus Workshop (E. H. D. Cameron, S. G. Hillier and K. Griffiths, eds.). Alpha Omega, Wales, UK. p. 223–225.Google Scholar
  3. Dray, F., J. E. Andrieu and F. Renaud (1975). Enzyme immunoassay of progesterone at the picogram level using β-galactosidase as label. Biochem. Biophys. Acta. 403: 131–138.PubMedGoogle Scholar
  4. Harpaz, N., H. M. Flowers and N. Sharon (1974). Purification of coffee bean ß-galactosidase by affinity chromatography. Biochem. Biophys. Acta. 341: 213–221.PubMedGoogle Scholar
  5. Kitagawa, T. and T. Aikawa (1976). Enzyme coupled immunoassay for insulin using a novel coupling reagent. J. Biochem. (Toyko) 79:233–236.Google Scholar
  6. Lai, H. Y. L. and B. Axelrod (1973). 1-Aminoglycosides, a new class of specific inhibitors of glycosides. Biochem. Biophys. Res. Commun. 54: 463–468.PubMedCrossRefGoogle Scholar
  7. Monji, N. and A. Castro (1979). Steric Hindrance Enzyme Immunoassay (SHEIA); a novel method in enzyme immunoassay. Res. Commun. Chem. Path. Pharm. 26: 187–196.PubMedGoogle Scholar
  8. Odell, W. D., C. Silver and P. K. Grover (1975). Competitive protein binding assays methods of spearation of bound from free, in Steroid Immunoassay, Proceeding of the Fifth Tenovus Workshop (E. H. D. Cameron, S. G. Hillier and K. Griffiths, eds.). Alpha Omega, Wales, UK. p. 207–222.Google Scholar
  9. Ratchliff, J. G. (1976). Radioimmunoassay and saturation analysis; separation techniques in saturation analysis, Brit. Med. Bull. 30: 32–37.Google Scholar
  10. Rubenstein, K. E., R. S. Schneider and E. F. Ullman (1972). “Homogeneous” enzyme immunoassay. A new immunochemical technique. Biochem Biophys. Res. Comm. 47: 846–851.PubMedCrossRefGoogle Scholar
  11. Steers, E. and P. Cuatrecasas (1974). β-Galactosidase. Methods in Enzymology 34: 350–358.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Albert Castro
    • 1
  • Nobuo Monji
    • 2
  1. 1.Department of PathologyUniversity of Miami School of MedicineMiamiUSA
  2. 2.Genetic Systems CorporationSeattleUSA

Personalised recommendations