Affinity Column Mediated Immunoenzymometric Assays
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Immunometric assays, by definition, are immunoassays configured in such a way that labeled-antibodies are utilized as the quantifying entity. This is in contrast to competitive radioimmunoassays or enzyme-immunoassays in which labeled-antigens are utilized in competition with sample antigen to bind to a limited number of antibody sites. The label applied to the antibody can take the form of a radioisotope, enzyme, fluorophore, luminescent tag, or almost any tag ultimately capable of generating a signal, even through some complex coupling mechanism. Two fundamentally different immunometric assay configurations have been described: the one-site immunometric assay and the two site (sandwich) immunometric assay.
KeywordsAffinity Column Unbind Fraction Immunometric Assay Crosslinking Reagent Control Pore Glass
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