Abstract
The current interest in immune regulation and in characterizing the immune response against pathogens and substances of interest to human and animal medicine, has prompted attempts to develop techniques capable of accurately and reliably measuring specific antibodies in serum and body fluids. Especially important is the ability to measure the response according to the isotype of the antibody. The enzyme-linked immunosorbent assay (ELISA) provides a sensitive and non-hazardous immunochemical basis for potentially making such determinations (Avrameas and Gilbert, 1972; Engvall et al. 1971). The amplified ELISA (a-ELISA) is a modification of the original ELISA which combines the principles of indirect detection with the use of a non-covalent enzyme-antibody detection system (Butler et al. 1978b). The latter avoids alteration of both enzyme and antibody, the complex in fact dissociating during the substrate reaction. The result is a system with improved sensitivity and broad flexibility. The a-ELISA serves as a useful means of measuring the isotypic distribution of specific immune responses in many species.
Researchon the a-ELISA has been most recently supported by Grants 83-CRSR-2-2172 (USDA), HL22676 (NIH) and 5T32 DE 07007 (NIH).
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© 1985 Plenum Press, New York
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Butler, J.E., Peterman, J.H., Koertge, T.E. (1985). The Amplified Enzyme-Linked Immunosorbent Assay (a-ELISA). In: Ngo, T.T., Lenhoff, H.M. (eds) Enzyme-Mediated Immunoassay. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5012-5_14
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DOI: https://doi.org/10.1007/978-1-4684-5012-5_14
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