Separation-Free Enzyme Fluorescence Immunoassay by Continuous Flow Injection Analysis
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Enzyme imnunoassays (EIAs) are now known to be a viable alter-native to the more established radioimmunoassay techniques. The inproved sensitivity along with the inherently better speed, stability, and cost have resulted in the commercial production of several EIA systems (Monroe, 1984). Most systems have colorimet-rically monitored the indicating enzyme reaction. With the appropriate considerations, a fluorescence monitor can further enhance the sensitivity and selectivity of such methods without introducing significant sacrifices in the ease, speed, or cost of analysis (Kelly and Christian, 1982).
KeywordsFlow Injection Analysis Fluorescence Immunoassay Flow Injection Analysis System Significant Sacrifice Imicating Reaction
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