Abstract
In man, the use of antigen-specific antibodies is an important clinical tool for diagnostic testing (e.g., blood typing for transfusion, tissue typing for transplantation) and for therapy (e.g., prophylaxis of Rh hemolytic disease of the newborn and zoster immune plasma) (Yankee et al., 1969; Grumet et al., 1982; Davey and Zipursky, 1979; Ross, 1962; Zaia et al., 1983). The limited availability or specificity of many of these reagents has placed an important restraint on their use. The production of hybrids between myeloma cell lines and lymphocytes from immunized hosts appears to make possible the unlimited production of monoclonal antibodies to predefined antigens (Köhler and Milstein, 1975). Successful application of this technique, primarily with rodents, is of limited clinical use because xenogeneic immunization with human cells mainly yields antibodies against monomorphic, species-specific antigens rather than polymorphic alloantigens. Moreover, rodent hybridomas have a theoretical limitation for therapeutic use because their secreted antibody would be treated by human recipients as a foreign protein with potential for inducing serum sickness.
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References
Arvin, A. M., and Koropchak, C. M., 1980, Immunoglobulins M and G to varicella-zoster virus measured by solid-phase radioimmunoassay: Antibody responses to varicella and herpes zoster infections, J. Clin. Microbial. 12:367.
Atkinson, K., Meyers J. D., Storb, R., Prentice, L., and Thomas, E, D., 1980, Varicella-zoster virus infection after marrow transplantation for aplastic anemia or leukemia, Transplantation 29:47.
Brodsky, F. M., Parham, P., Barnstable, C. J., Crumpton, M. J., and Bodmer, W. F., 1979, Monoclonal antibodies for analysis of the HLA system, Immunol. Rev. 47:3.
Croce, C. M., Linnenbach, A., Hall, W., Steplewski, Z., and Koprowski, H., 1980, Production of human hybridomas secreting antibodies to measles virus, Nature 288:488.
Davey, M. G., and Zipursky, A., 1979, McMaster Conference in Prevention of Rh Immunization, Vox Sang. 36:50.
De-The, G., 1982, Epidemiology of Epstein-Barr virus and associated diseases in man, In: The Herpesviruses, Volume 1, B. Roizman, ed., Plenum Press, New York, p. 25.
Feldman, S., Hughes, W. T., and Daniel, C. B., 1975, Varicella in children with cancer: Seventy-seven cases, Pediatrics 56:388.
Foung, S. K. H., Sasaki, D., Grumet, F. C., and Engleman, E. G., 1982, Production of functional human T-T hybridomas in selection medium lacking aminopterin and thymidine, Proc. Natl. Acad. Sci. USA 79:7484.
Foung, S. K. H., Perkins, S., Raubitschek, A., Larrick, J., Lizak, G., Fishwild, D., Engleman, E. G., and Grumet, F. C. 1984, Rescue of human monoclonal antibody production from an EBV transformed B cell line by fusion to a human—mouse hybridoma, J. Immunol. Meth. 70:83.
Grose, C., Edmond, B. J., and Brunell, P. A., 1979, Complement enhanced neutralizing antibody response to varicella-zoster virus, J. Infect. Dis. 139:432.
Grose, C., Edwards, D. P., Friedrichs, W. E., Weigle, K. A., and McGuire, W. L., 1983, Monoclonal antibodies against three major glycoproteins of varicella-zoster virus, Infect. Immunol. 40:381.
Grose, C., Edwards, D. P., Weigle, K. A., Friedrichs, W. E., and McGuire, W. L., 1984, Varicella-zoster virus-specific gp 140: A highly immunogenic and disulfide-linked structural glycoprotein, Virology 132:138.
Grumet, F. C., Fendly, B. M., Fish, L., Foung, S., and Engleman, E. G., 1982, A monoclonal antibody (B27M2) subdividing HLA-B27, Hum. Immunol. 5:61.
Henle, W., and Henle, G., 1982, Immunology of Epstein-Barr virus, In: The Herpesviruses, Volume 1, B. Roizman, ed., Plenum Press, New York, p. 209.
Hunter, S. W., Fujiwara, T., and Brennan, P. J., 1982, Structure and antigenicity of the major specific glycolipid antigen of Mycobacterium leprae,J. Biol. Chem. 257:15072.
Köhler, G., and Milstein, C., 1975, Continuous cultures of fused cells secreting antibody of predefined specificity, Nature 256:495.
Lane, H. C., Shelhomer, J. H., Mostowski, H. S., and Fauci, A. S., 1982, Human monoclonal anti-keyhole limpet hemocyanin antibody-secreting hybridoma produced from peripheral blood B lymphocytes of a keyhole limpet hemocyanin-immune individual, J. Exp. Med. 155:333.
Meyers, J. D., Flournoy, N., and Thomas, E. D., 1980, Cell-mediated immunity to varicella-zoster virus after allogeneic marrow transplant, J. Infect. Dis. 141:479.
Nowinski, R., Boglund, C., Lane, J., Lastrum, M., Bernstein, I., Young, W., Hakomori, S., Hall, L., and Cooney, M., 1980, Production of antibody to tetanus toxoid by continuous human lymphoblastoid cell lines, Science 199:1439.
Olsson, L., and Kaplan, H. S., 1980, Human-human hybrids producing monoclonal antibodies of predefined antigenic specificity, Proc. Natl. Acad. Sci. USA 77:5429.
Parker, J., Marcoux, D. A., Hafleigh, E. B., and Grumet, F. C., 1978, Modified microtiter tray method for blood typing, Transfusion 18:417.
Ross, A. H., 1962, Modification of chickenpox in family contacts by administration of gamma globulin, N. Engl. J. Med. 267:369.
Schlom, J., Wunderlich, D., and Teramoto, Y. A., 1980, Generation of human monoclonal antibodies reactive with human mammary carcinoma cells, Proc. Natl. Acad. Sci. USA 77:6841.
Sly, W. S., Sekhon, G. S., Kennett, R., Bodmer, W. F., and Bodmer, J., 1976, Permanent lymphoid lines from genetically marked lymphocytes: Success with lymphocytes recovered from frozen storage, Tiss. Antigens 7:165.
Steinitz, M., Klein, G., Koskimies, S., and Makela, O., 1977, EB virus-induced B lymphocyte cell lines producing specific antibodies, Nature 269:420.
Takahashi, M., Otsuka, T., Okuno, Y., Asano, Y., Yazaki, T., and Isomura, S., 1974, Live vaccine used to prevent the spread of varicella in children in hospital, Lancet 2:1288.
Tamura, G. S., Dailey, M. O., Gallatin, W.M., McGrath, M. S., Weissman, I. L., and Pillemer, E. A., 1984, Isolation of molecules recognized by monoclonal antibodies and antisera: The solid phase immunoisolation technique, Anal. Biochem. 136:458.
Watson, J. G., 1983, Problems of infection after bone marrow transplantation, J. Clin. Pathol. 36:683.
Weller, T. H., 1983, Varicella and herpes zoster, N. Engl. J. Med. 309:1362.
Yankee, R. A., Grumet, F. C., and Rogentine, G. N., 1969. The selection of compatible platelet donors for refractory patients by lymphocyte HLA typing, N. Engl. J. Med. 281:1208.
Zaia, J. A., Levin, M. J., Preblud, S. R., Leszczynski, J., Wright, G. G., LeGore, J., Ellis, R. J., Curtis, A. C., and Valerio, M.A., 1983. Evaluation of varicella-zoster immune globulin: Protection of immunosuppressed children after household exposure to varicella, J. Infect. Dis. 147:737.
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© 1985 Plenum Press, New York
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Foung, S.K.H. et al. (1985). Production of Human Monoclonal Antibodies Using a Human-Mouse Fusion Partner. In: Engleman, E.G., Foung, S.K.H., Larrick, J.W., Raubitschek, A.A. (eds) Human Hybridomas and Monoclonal Antibodies. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4949-5_8
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DOI: https://doi.org/10.1007/978-1-4684-4949-5_8
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