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Human Hybridomas and Monoclonal Antibodies pp 385–400Cite as

Human T-T Hybridomas Specific for Epstein—Barr Virus

Generation and Function

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Abstract

Two technologies currently exist to obtain clonal expression of human T cells. One relies on interleukin 2 (IL-2) to expand single-cell cultures. This creates lines of T cells whose maintenance remains totally dependent on the presence of the lymphokine. The second approach, somatic cell hybridization, generates clonal populations of T cells capable of autonomous growth. Successful hybridization is crucially dependent on the phenotype, functional properties, and growth characteristics of the parental cell lines. By appropriate matching of an immortal malignant T-cell line with a normal T cell prior to fusion, a hybrid can be created that expresses a specific molecule or activity in an antigen-specific or -nonspecific manner. The technique has its limitations, but it has resulted in initial successes.

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References

  • Asada, M., Higuichi, M., Kobayashi, Y. and Osawa, T., 1983, Human T-cell hybridomas producing lymphokines. I. Enchancement of lymphotoxin secretion from human T-cell hybridomas by phorbol myristate acetate, Cell. Immunol. 77: 150–160.

    Article  PubMed  CAS  Google Scholar 

  • Butler, J. L., Muraguchi, A., Lane, H. C., and Fauci, A. S., 1983, Development of a human T-T cell hybridoma secreting B cell growth factor, J. Exp. Med. 157: 60–68.

    Article  PubMed  CAS  Google Scholar 

  • Carson, D. A., Kaye, J., and Seegmiller, J. E., 1978, Differential sensitivity of human leukemic T cell lines and B cell lines to growth inhibition by deoxyadenosine, J. Immunol. 121: 1726–1731

    PubMed  CAS  Google Scholar 

  • DeFreitas, E. C., Vella, S., Linenbach, A., Zimjewski, C., Koprowski, H., and Croce, C. M., 1982, Antigen-specific human T-cell hybridomas with helper activity, Proc. Natl. Acad. Sci. USA 79: 6646–6650.

    Article  PubMed  CAS  Google Scholar 

  • Epstein, M. A., and Achong, G. B., 1977, Recent progress in Epstein—Barr virus research, Annu. Rev. Microbiol. 31: 421–445.

    Article  PubMed  CAS  Google Scholar 

  • Foung, S. K., Saski, D. T., Grumet, F. C., and Engleman, E. G., 1982, Production of functional human T-T hybridomas in selection medium lacking aminopterin and thymidine, Proc. Natl. Acad. Sci. USA 79: 7484–7488.

    Article  PubMed  CAS  Google Scholar 

  • Gallagher, G., and Stimson, W. H., 1983, Generation of human T-cell hybrids with the characteristics of human peripheral blood T-lymphocytes, Immunol. Lett. 6: 203–207.

    Article  PubMed  CAS  Google Scholar 

  • Gillis, S., and Watson, J., 1980, Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin-2 producing human leukemia T cell line, J. Exp. Med. 152: 1709–1719.

    Article  PubMed  CAS  Google Scholar 

  • Gillis, S., Ferm, M. M., Ou, W., and Smith, K. A., 1978, T-cell growth factor: Parameters of production and a quantitative microassay for activity, J. Immunol. 120: 2027–2032.

    PubMed  CAS  Google Scholar 

  • Greene, W. C., Fleisher, T. A., Nelson, D. L., and Waldmann, T. A., 1982, Production of human suppressor T cell hybridomas, J. Immunol. 129: 1986–1991.

    PubMed  CAS  Google Scholar 

  • Greene, W. C., Depper, J. M., Leonard, W. J., Fleisher, T. A., and Waldmann, T. A., 1983, Construction of human suppressor T cell hybridomas, Lymphokine Res. 2 (2): 53–60.

    PubMed  CAS  Google Scholar 

  • Grillot-Courvalin, C., Brouet, J., Berger, R., and Bernheim, A., 1981, Establishment of human Tcell hybrid line with suppressive activity, Nature 292: 844–845.

    Article  PubMed  CAS  Google Scholar 

  • Huff, T. F., and Isbizaka, K., 1984, Formation of IgE-binding factors by human T-cell hybridomas, Proc. Natl. Acad. Sci. 81: 1514–1518.

    Article  PubMed  CAS  Google Scholar 

  • Irigoyen, O., Rizzolo, P. V., Thomas, Y., Rogozinski, L., and Chess, L., 1981, Generation of functional human T-cell hybrids, J. Exp. Med. 154: 1827–1837.

    Article  PubMed  CAS  Google Scholar 

  • Kappler, J. W., Skidmore, B., White, J., and Marrack, P., 1981, Antigen-inducible, H-2 restricted, interleukin-2-producing T-cell hybridomas, J. Exp. Med. 153: 1198–1214.

    Article  PubMed  CAS  Google Scholar 

  • Klebe, R. J., and Mancuso, M. G., 1981, Chemicals which promote cell hybridization, Somat. Cell Genet. 7 (4): 473–488.

    Article  PubMed  CAS  Google Scholar 

  • Kobayashi, Y., Asada, M., Higuchi, M., and Osawa, T., 1982, Human T-cell hybridomas producing lymphokines. I. Establishment and characterization of human T-cell hybridomas producing lymphotoxin and migration inhibitory factor, J. Immunol. 128: 2714–2718.

    PubMed  CAS  Google Scholar 

  • Lakow, E., Tsoukas, C. D., Vaughan, J. H., Altman, A., and Carson, D. A., 1983, Human T-cell hybridomas specific for Epstein—Barr virus-infected B lymphocytes, J. Immunol. 130: 169–172.

    PubMed  CAS  Google Scholar 

  • Lakow, E. S., Valentine, M. A., Vaughan, J. H., Tsoukas, C. D., and Carson, D. A., 1984, Effects of monoclonal antibodies against lymphocyte surface antigens on interleukin-2 excretion by Epstein—Barr virus-specific human T cell hybridomas, Cell Immunol. 85: 67–74.

    Article  PubMed  CAS  Google Scholar 

  • Le, J., Vilcek, J., Adlik, J. R., Cheung, M. K., Balazs, I., Sarngadharan, M. G., and Prensky, W., 1983, Lymphokine production by human T cell hybridomas, J. Immunol. 130: 1231–1235.

    PubMed  CAS  Google Scholar 

  • Mayer, L., Fu, S. M., and Kunkel, H. G., 1982, Human T-cell hybridomas secreting factors for IgAspecific help, polyclonal B cell activation, and B cell proliferation, J. Exp. Med. 156: 1860–1865.

    Article  PubMed  CAS  Google Scholar 

  • Murakami, M., and Cathcart, M. K., 1984, Human thymus cell hybridoma secreting a nonspecific immuno-suppressor: participation of T4 positive T lymphocytes and monocytes, Fed. Proc. 43 (6): 1500 (abstract).

    Google Scholar 

  • Okada, M., Yoshimura, N., Kaieda, T., Yamamura, Y., and Kishimoto, T., 1981, Establishment and characterization of human T hybrid cells secreting immunoregulatory molecules, Proc. Natl. Acad. Sci. USA 78: 7717–7721.

    Article  PubMed  CAS  Google Scholar 

  • Osawa, T., Kobayashi, Y., Asada, M., and Higuchi, M., 1983, Lymphokine-producing human T-cell hybridomas, in: Humoral Factors in Host Defense (Y. Yamamura, ed.), Academic Press, New York, pp. 291–306.

    Google Scholar 

  • Platsoucas, C. D., Calvelli, T. A., Kunicka, J. E., Lawless, B. D., and Higgins, J. A., 1984, Constitutive production of a suppressor factor by human T-T cell hybridomas, Fed. Proc. 43 (6): 1605 (abstract).

    Google Scholar 

  • Ruscetti, F. W., and Gallo, R. C., 1981, Human T-lymphocyte growth factor: Regulation of growth and function of T lymphocytes, Blood 57: 379–394.

    PubMed  CAS  Google Scholar 

  • Schachtschabel, D. O., Lazarus, H., Farber, S., and Foley, G. E., 1966, Sensitivity of cultured human lymphoblasts (CCRF-CEM cells) to inhibition by thymidine, Exp. Cell Res. 43: 512–514.

    Article  PubMed  CAS  Google Scholar 

  • Schwenk, H., and Schneider, U., 1975, Cell cycle dependency of a T-cell marker on lymphoblasts, Blut 31: 299–306.

    Article  PubMed  CAS  Google Scholar 

  • Theodore, A. C., Beer, D. J., Picarella, D. E., Rosenwasser, L. J., and Center, D. M., 1984, Production of lymphotactic lymphokines by human T-T cell hybridomas, Fed. Proc. 43 (6): 1603 (abstract).

    Google Scholar 

  • Thompson, L. H., and Baker, R., 1973, Isolation of mutants of cultured mammalian cells, in: Methods in Cellular Biology ( D. A. Prescott, ed.), Academic Press, New York, pp. 209–281.

    Google Scholar 

  • Tsoukas, C. D., Fox, R. I., Slovin, S. F., Carson, D. A., Pelligrino, M., Fong, S., Pasquali, J. L., Ferrone, S., Kung, P., and Vaughan, J. H., 1981, T lymphocyte mediated cytotoxicity against autologous EBV-genome-bearing B cells, J. Immunol. 126: 1742–1746.

    PubMed  CAS  Google Scholar 

  • Valentine, M. A., Lakow, E. S., Vaughan, J. H., and Carson, D. A., 1983, T cell hybridomas: A new approach for studying the functional properties of T cells in rheumatoid arthritis, Arthritis Rheum. 26: 545 (abstract).

    Google Scholar 

  • Valentine, M. A., Tsoukas, C. D., Vaughan, J. H., and Carson, D. A., 1984, Human T cell hybrids expressing naturally occurring HLA-DR restricted receptors that recognize EBV-infected B cells, J. Cell. Biochem. (Suppl.) 8A: 275 (abstract).

    Google Scholar 

  • Vervliet, G., Asma, G., Vossen, J., and Billiau, A., 1983, Isolation and characterization of a human HAT-sensitive T-cell line as a tool for the construction of human T-cell hybridomas, Arch. Int. Physiol. Biochim. 90 (A): B224–225.

    Google Scholar 

  • Weiser, W., Kawaguchi, T., David, J., and Remold, H., 1984, Generation of human hybridomas producing MIF and of murine hybridomas secreting monoclonal antibodies to these MIF species, Fed. Proc. 43 (6): 1500 (abstract).

    Google Scholar 

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Authors and Affiliations

  1. Scripps Clinic and Research Foundation, La Jolla, California, 92037, USA

    Mary A. Valentine & Dennis A. Carson

Authors
  1. Mary A. Valentine
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  2. Dennis A. Carson
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Editors and Affiliations

  1. Department of Pathology, Stanford University School of Medicine, Stanford University Medical Center, 94305, Stanford, California, USA

    Edgar G. Engleman  & Steven K. H. Foung  & 

  2. Cetus Immune Research Laboratories, 94303, Palo Alto, California, USA

    James W. Larrick  & Andrew A. Raubitschek  & 

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© 1985 Plenum Press, New York

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Valentine, M.A., Carson, D.A. (1985). Human T-T Hybridomas Specific for Epstein—Barr Virus. In: Engleman, E.G., Foung, S.K.H., Larrick, J.W., Raubitschek, A.A. (eds) Human Hybridomas and Monoclonal Antibodies. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4949-5_23

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  • DOI: https://doi.org/10.1007/978-1-4684-4949-5_23

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-4951-8

  • Online ISBN: 978-1-4684-4949-5

  • eBook Packages: Springer Book Archive

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