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The Production of Monoclonal Antibodies by Human—Human Hybridomas

Their Application to Studies of Autoimmune Diseases

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Human Hybridomas and Monoclonal Antibodies

Abstract

In this chapter we review our experience with the human—human hybridoma technology and compare our results with those of others. We also provide a brief review of our results in the analysis of human monoclonal lupus autoantibodies. A more comprehensive review of applications of hybridoma technology to studies of human autoimmune disease has been published elsewhere (Shoenfeld and Schwartz, 1984). Anyone just beginning to use the hybridoma method with human lymphocytes should appreciate that the technology is in a state of flux. Many of the methodological details are bound to change as new fusion partners and other improvements become introduced. Nothing written here should be considered as the final word on the topic.

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Author information

Authors and Affiliations

  1. Department of Medicine “D” (Research Laboratory of Autoimmune Diseases), Beilinson Medical Center, Petach Tiqva, Israel

    Yehuda Shoenfeld

  2. Hematology-Oncology Division, New England Medical Center, Boston, Massachusetts, 02111, USA

    Robert S. Schwartz

Authors
  1. Yehuda Shoenfeld
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  2. Robert S. Schwartz
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Editor information

Editors and Affiliations

  1. Department of Pathology, Stanford University School of Medicine, Stanford University Medical Center, 94305, Stanford, California, USA

    Edgar G. Engleman  & Steven K. H. Foung  & 

  2. Cetus Immune Research Laboratories, 94303, Palo Alto, California, USA

    James W. Larrick  & Andrew A. Raubitschek  & 

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© 1985 Plenum Press, New York

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Shoenfeld, Y., Schwartz, R.S. (1985). The Production of Monoclonal Antibodies by Human—Human Hybridomas. In: Engleman, E.G., Foung, S.K.H., Larrick, J.W., Raubitschek, A.A. (eds) Human Hybridomas and Monoclonal Antibodies. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4949-5_14

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  • DOI: https://doi.org/10.1007/978-1-4684-4949-5_14

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-4951-8

  • Online ISBN: 978-1-4684-4949-5

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