Abstract
Our laboratories have been using two-dimensional gel electrophoresis and monoclonal antibody techniques for the study of contractile and structural proteins. The resolving power of the two-dimensional gels, combined with computer methods for quantitation and pattern matching, have been used in previous studies to analyze the proteins of cultured L6 muscle cells as they differentiate from myoblasts to myotubes (453,497,498). Highly specific monoclonal antibodies have made possible the identification and biochemical characterization of major and minor structural proteins. Matsumura et al (1983) have used the monoclonal antibodies to tropomyosins to identify 5 isoforms that are present in several rat cell lines (499). These results confirm and extend the earlier identification of tropomyosin isoforms in L6 myoblasts (453). Matsumura et al (1983) have further shown that actin-filament assemblies can be purified from non-muscle and muscle cells as ordered bundles cross-linked in a highly regular fashion by monoclonal antibodies to tropomyosin. (500).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1985 Plenum Press, New York
About this chapter
Cite this chapter
Garrels, J.I., Yamashiro-Matsumura, S., Lin, J.JC., Matsumura, F. (1985). A Set of Actin-Filament Associated Proteins Characterized by Quantitative Two-Dimensional Gel Electrophoresis. In: Strohman, R.C., Wolf, S. (eds) Gene Expression in Muscle. Advances in Experimental Medicine and Biology, vol 182. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4907-5_19
Download citation
DOI: https://doi.org/10.1007/978-1-4684-4907-5_19
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4909-9
Online ISBN: 978-1-4684-4907-5
eBook Packages: Springer Book Archive