Secondary CO2 Diffusion Following HCO3- Shift Across the Red Cell Membrane

  • Masaji Mochizuki
  • Tomoko Kagawa
  • Kyuichi Niizeki
  • Akito Shimouchi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 180)


In order to elucidate the effect of CO2 diffusion in the red blood cell (RBC) on the Bohr shift, it is absolutely necessary to evaluate the diffusivity of CO2 and HCO3 within the RBC as well as across the RBC membrane. Uchida et al.(1) measured the diffusion coefficients of CO2 and HCO 3 - ions in hemoglobin solution (Hb) in a Hb layer with a constant thickness by varying Hb concentration. Extrapolating the relation between the diffusion coefficient and Hb concentration, the coefficients of CO2 and HCO 3 - within the RBC were estimated as follows: D(CO2) = 3.4 × 10-6, and D(HCO 3 - ) = 1.4 × 10-6 cm2/sec, respectively. Furthermore, Niizeki et al. (2) measured the diffusion rate of CO2 into the RBC by using a stopped flow method, where the HCO 3 - shift was suppressed by contrplling intracellular Cl- concentration preliminarily. The half-time, being about 77 msec, was longer than the values measured by a rapid flow method by Pilper (3), and Constantine et al.(4). From Niizeki’s data the diffusivity across the boundary layer, or the transfer coefficient of CO2, η(CO2) was estimated to be; η(CO2) = 2 × 10-6 cm/sec • Torr, on an average. From our microphotometric observation it was confirmed that the Bohr shift does not occur in a buffer solution.


Transfer Coefficient Diffusion Equation Outward Diffusion Hemoglobin Solution Alternate Direction Implicit Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    K. Uchida, M. Mochizuki, and K. Niizeki, Diffusion coefficients of CO2 molecule and bicarbonate ion in hemoglobin solution measured by fluorescence technique. Jap. J. Physiol. 33(4), in press (1983).Google Scholar
  2. 2.
    K. Niizeki, M. Mochizuki, and K. Uchida, Rate of CO2 diffusion in the human red blood cell measured with pH sensitive fluorescence. Jap. J. Physiol. 33(4), in press (1983).Google Scholar
  3. 3.
    J. Piiper, Geschwindigkeit des CO2 Austausches zwischen Erythrocyten und Plasma. Pflügers Arch. 278, 500–512 (1964).CrossRefGoogle Scholar
  4. 4.
    H. P. Constantine, M. R. Craw, and R. E. Forster, Rate of the reaction of carbon dioxide with human red blood cells. Am. J. Physiol. 208, 801–811 (1965).PubMedGoogle Scholar
  5. 5.
    R. A. Klocke, Rate of bicarbonate-chloride exchange in human red cells at 37°C. J. Appl. Physiol. 40, 707–714 (1976).PubMedGoogle Scholar
  6. 6.
    G. Thews, Die Sauerstoffdiffusion in den Lungencapillaren. In: Physiologie und Pathophysiologie des Gasaustausehes in der Lunge, ed. by Bartels, H. and Witzleb, E. Springer Verlag, Berlin and Heidelberg, pp 1–19 (1961).CrossRefGoogle Scholar
  7. 7.
    H. Takiwaki, M. Mochizuki, and K. Niizeki, Relationship between hematocrit and CO2 contents in whloe blood and true plasma. Jap. J. Physiol. 33(4), in press (1983).Google Scholar
  8. 8.
    M. Mochizuki, H. Takiwaki, T. Kagawa, and H. Tazawa, Derivation of theoretical equations of the CO2 dissociation curve and the carbamate fraction in the Haldane effect. Jap. J. Physiol. 33(4), in press (1983).Google Scholar
  9. 9.
    H. Tazawa, M. Mochizuki, M. Tamura, and T. Kagawa, Quantitative analyses of the CO2 dissociation curve of oxygenated blood and the Haldane effect in human blood. Jap. J. Physiol. 33(4), in press (1983).Google Scholar
  10. 10.
    D. D. Van Slyke, H. Wu, and F. C. Mclean, Studies of gas and electrolyte equilibria in the blood. V. Factors controlling the electrolyte and water distribution in the blood. J. Biol. Chem. 106, 765–849 (1923).Google Scholar
  11. 11.
    T. Kagawa, and M. Mochizuki, Numerical solution of partial differential equation describing oxygenation rate of the red blood cell. Jap. J. Physiol. 32, 197–218 (1982).CrossRefGoogle Scholar
  12. 12.
    R. E. Forster, and E. D. Crandall, Time course of exchanges between red cells and extracellular fluid during CO o uptake. J. Appl. Physiol. 38, 710–718 (1975).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Masaji Mochizuki
    • 1
  • Tomoko Kagawa
    • 1
  • Kyuichi Niizeki
    • 1
  • Akito Shimouchi
    • 1
  1. 1.Department of PhysiologyYamagata University School of MedicineYamagataJapan

Personalised recommendations