Increased Resolution Power in Po2 Analysis at Lower Po2 Levels Via Sensitivity Enhanced Optical Po2 Sensors (Po2 Optodes) using Fluorescence Dyes

  • N. Opitz
  • D. W. Lübbers
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 180)


Po2 optodes (Lübbers and Opitz, 1975) are based on fluorescence quenching of suitable indicator molecules, e.g. pyrenebutyric acid (PBA) (Vaughan and Weber, 1970; Knopp and Longmuir, 1972), by molecular oxygen within a membrane or boundary layer protected indicator film of about 10/um thickness. Fig. 1 shows a couple of O2-depen-dent corrected excitation spectra of PBA (λ em = 395 nm) in a silisensitivity is drastically enhanced at lower PO2 values for the indicator membrane, whereas for PO2 values > 100 Torr the sensitivity of the indicator solution would be more favourable.


Silicone Membrane Quenching Constant Indicator Solution Broad Band Excitation Indicator Molecule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Boldt, M., 1971, Ein korrigiert messendes Spektrofluorometer zur Messung an trüben Medien, Dissertation, Marburg.Google Scholar
  2. Knopp, J.A., and I.S. Longmuir, 1972, Intracellular measurement of oxygen by quenching of fluorescence of pyrenebutyric acid, Biochim. Biophys. Acta, 279:393–397.PubMedCrossRefGoogle Scholar
  3. Lubbers, D.W., and Opitz, N., 1975, Die pCO2/pO2-Optode: eine neue pCO2-bzw. pO2-Meßsonde zur Messung des pCO2 oder pO2 von Gasen und Flüssigkeiten, Z. Naturforsch., 30c:532–533.Google Scholar
  4. Opitz, N., and Lübbers, D.W., 1982, Evidence for boundary layer effects influencing the sensitivity of microencapsulated O2 fluorescence indicator molecules in: Oxygen Transport to Tissue V, D.W. Lübbers, H. Acker, T.K. Goldstick, E. Leniger-Follert, eds., Plenum Press, New York, in press.Google Scholar
  5. Opitz, N., and D.W. Lübbers, 1983, Hochauflösende PO2-Analyse mit abstimmbaren Fluoreszenzmeßsonden, Biomed. Technik, 31(28):Google Scholar
  6. Opitz, N., and Lübbers, D.W., 1983, Compact CO2 gas analyzer with favourable SNR and resolution using special fluorescence sensors (optodes) illuminated by blue LED’S, this volumeGoogle Scholar
  7. Stern, O., and Volmer, M., 1919, Über die Abklingzeit der Fluorezenz, Z. Phys., 20:183.Google Scholar
  8. Vaughan, W.M., and Weber, G., 1970, Oxygen quenching of pyrenebutyric acid fluorescence in water. A dynamic probe of the microenvironment, Biochem., 9:464.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • N. Opitz
    • 1
  • D. W. Lübbers
    • 1
  1. 1.Max-Planck-Institut für SystemphysiologieDortmundGermany

Personalised recommendations