Abstract
Each organ system of the mammalian anatomy is recognized as performing a unique or specific function in the overall biological activity of the organism. Every organ, in turn, also consists of several cell types, each with often divergent and unique properties that collectively carry out the functions of the particular organ. One approach to investigate the biochemical and physiological properties is to isolate and perfuse the organ with an extracorporeal system.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
A. L. Chute and D. H. Smyth, Metabolism of the isolated perfused cat’s brain, Quart, J. Exp, Physiol, 27:379 (1939).
L. R. Drewes and D. D. Gilboe, Nutrient transport systems in dog brain, Fed, Proc. 36:166 (1977).
D. D. Gilboe, Perfusion of the isolated brain, in: “Handbook of Neurochemistry,” Vol. 2 (2nd Ed.), A. Lajtha, ed., Plenum Press, NY (1983).
D. D. Gilboe, A. L. Betz, and L. R. Drewes, Use of the isolated canine brain in studies of cerebral metabolism, metabolite transport and cerebrovascular physiology, in: “Research Methods in Neurochemistry,” N. Marks and A. Rodnight, eds., Plenum Press, NY (1975).
L. R. Drewes, An improved apparatus for blood perfusion of the canine cerebral vasculature, Neurochem. Res. 5:553 (1980).
B. L. Nazar and A. Schoolwerth, An improved microfluorometric enzymatic assay for the determination of ammonia, Anal. Bio-chem. 95:507 (1979).
H. Honorst, Lactate analysis, in: “Methods in Enzymatic Analysis, “ H. Bergmeyer ed., Academic Press, NY (1965).
A. L. Betz, D. D. Gilboe, D. L. Yudilevich, and L. R. Drewes, Kinetics of unidirectional glucose transport into the isolated dog brain, Am. J. Physiol. 225:586 (1973).
L. R. Drewes and D. D. Gilboe, Glycolysis and the permeation of glucose and lactate in the isolated, perfused dog brain during anoxia and postanoxic recovery, J. Biol. Chem. 248:2489 (1973).
R. A. Hartwick and P. R. Brown, The performance of microparticle chemically-bonded anion-exchange resins in the analysis of nucleotides, J. Chromatogr. 112:651 (1975).
S. S. Kety, Blood-tissue exchange methods, Meth. Med. Res. 8:223 (1960).
O. Sakurada, C. Kennedy, J. Jehle, J. D. Brown, G. L. Carbin, and L. Sokoloff, Measurement of local cerebral blood flow with iodo[14C]antipyrine, Am. J. Physiol. 234:H59 (1978).
R. Hawkins, W. K. Hass, and J. Ransohoff, Measurement of regional brain glucose utilization in vivo using [2-14C]glucose, Stroke 10:690 (1979).
L. Sokoloff, M. Reivich, C. Kennedy, M. Des Rosiers, C. Patlak, K. Pettigrew, O. Sakurada, and M. Shinohara, The [14C]deoxy-glucose method for the measurement of local cerebral glucose utilization, J. Neurochem. 28:897 (1977).
J. Fitzpatrick, D. D. Gilboe, L. R. Drewes, and A. L. Betz, Relationship of cerebral oxygen uptake to EEG frequency in isolated canine brain, Am. J. Physiol. 231:1840 (1976).
W. H. Oldendorf, Carrier-mediated blood-brain transport of shortchain monocarboxylic organic acids, Am. J. Physiol. 224:1450 (1973).
M. Cavazutti and T. E. Duffy, Regulation of local cerebral blood flow in normal and hypoxic newborn dogs, Ann. Neurol. 11:247 (1982).
T. E. Duffy, M. Cavazzuti, N. Cruz, and L. Sokoloff, Local cerebral glucose metabolism in newborn dogs: Effects of hypoxia and halothane anesthesia, Ann. Neurol. 11:233 (1982).
A. L. Betz, D. D. Gilboe, and L. R. Drewes, Effects of anoxia on net uptake and unidirectional transport of glucose into the isolated dog brain, Brain Res. 67:307 (1974).
J. Jacquez, Modulation of glucose transport in human red blood cells by ATP, Biochim. Biophys. Acta 727:367 (1983).
A. Cooper, J. M. McDonald, A. Gelbard, R. Gledhill, and T. E. Duffy, The metabolic fate of 13N-labeled ammonia in rat brain, J. Biol. Chem. 254:4982 (1979).
B. K. Siesjö, “Brain Energy Metabolism,” John Wiley, NY (1978).
D. D. Gilboe and A. L. Betz, Oxygen uptake in the isolated canine brain, Am. J. Physiol. 224:588 (1973).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Plenum Press, New York
About this chapter
Cite this chapter
Drewes, L.R., Singh, A.K. (1984). Perfused Canine Brain: Metabolism and Blood-Brain Transport During Altered Metabolic States. In: Bruley, D., Bicher, H.I., Reneau, D. (eds) Oxygen Transport to Tissue—VI. Advances in Experimental Medicine and Biology, vol 180. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4895-5_12
Download citation
DOI: https://doi.org/10.1007/978-1-4684-4895-5_12
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4897-9
Online ISBN: 978-1-4684-4895-5
eBook Packages: Springer Book Archive