Abstract
A new erythroleukemic line, designated IDA, has been established from a non-lymphoid acute leukemia of M6 type. This line is characterized by a doulgling time of 35.9 ± 2.5 h and a saturation density of ~ 3 x 106 cells/ml. Interestingly it is essentially euployd (modal No. of chromosomes, 46), although it may generate occasionally polyploid cells and elements with aberrations often involving chromosome 21. When treated with hemin ± DMSO, cells are induced to massive erythroid dfferentiation (up to ≧ 90% benzidine positive cells). The IDA line is characterized by spontaneous and induced production of embryonic ς- and ε-globin chains, as evaluated by analytical isoelectric-focusing of the haptoglobin-Sepharose purified globin fraction.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Ferrero, D. and Rovera, G. (1984) Clinics in Hematology, in press.
Lozzio, C.B. and Lozzio, B.B. (1975) Blood, 45, 321–330.
Martin, P. and Papayannopoulou, T. (1982) Science 216, 1233–1235.
Lozzio, B.B., Lozzio, C.B., Bamberger, E.G. and Felin, A.S. (1981) Proc. Soc. Exp. Biol. Med. 166, 546–550.
Reuben, R.C., Rifkind, R.A., Marks, P.A. (1980) BBA Rev. Cancer 605, 325–331.
Anderson, I.C., Jokinen, M. and Gahmberg, C.G. (1979) Nature, 278, 364–366.
Rutherford, T.R., Clegg, J.B. and Weatherall D.J. (1979) Nature, 280, 164–165.
Cioè, L., McNab, A., Hubbell, H.R., Meo, P., Curtis, P. and Rovera, G. (1981) Cancer Res. 41, 237–247.
Rutherford, T., Clegg, J.B., Higgs, D.R., Jones, R.W., Thompson, J. and Weatherall, D.J. (1981) Proc. Natl. Acad. Sci. USA 78, 348–352.
Charnay, P., Maniatis, T. (1983) Science 220, 1281–1283.
Marie, J.P., Izaguirre, C.A., Civin, C.I., Mirro, J. and McCulloch, E.A. (1981) Blood 58, 708–711.
Gewirtz, A.M., Burger, D., Rado, T.A., Benz, E.J. and Hoffman,R. (1982) Blood 60, 785–789.
Sandoz, S.A., Bale, Atlas of Hematology, 1972.
Gianni, A.M., Presta, M., Polli, E., Saglio, G., Lettieri, F., Peschle, C., Comi, P., Giglioni, B. and Ottolenghi, S. (1982) In “Advances in Red Cell Biology” Weatherall, D.J. et al., eds., Raven Press, New York, pp. 279–287.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Plenum Press, New York
About this chapter
Cite this chapter
Migliaccio, G. et al. (1984). A New Human Erythroleukemic Line: Initial Characterization and Hemin-Induced Erythroid Differentiation. In: Aaronson, S.A., Frati, L., Verna, R. (eds) Genetic and Phenotypic Markers of Tumors. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4856-6_31
Download citation
DOI: https://doi.org/10.1007/978-1-4684-4856-6_31
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4858-0
Online ISBN: 978-1-4684-4856-6
eBook Packages: Springer Book Archive