Abstract
Monoclonal antibodies have been generated that are reactive with human colon carcinomas. The rationale for the studies was to utilize extracts from patient biopsy material (and not colon cancer cell lines) as immunogen to increase the probability that any monoclonal antibody generated be reactive with colon carcinomas in a clinical setting. Five immunization protocols were used employing extracts and membrane enriched fractions from both primary and metastatic colon carcinoma lesions. Twenty nine monoclonal antibodies from double-cloned hybridoma cultures have been characterized; all are of the IgG isotope. Preliminary results indicate that the monoclonal antibodies can be placed into 15 groups on the basis of their differential reactivities to six colon carcinoma extracts, the surface of three colon carcinoma cell lines and five partially purified CEA preparations from bloods of colon cancer patients. Some of the monoclonal antibodies were shown to bind from one to five of the five CEA preparations, while others showed no anti-CEA reactivity. None of the 29 monoclonal antibodies reacted with extracts of 21 normal human tissues including livers, spleens, kidneys, red blood cells, (of several blood groups), or polymorphonuclear leucocytes. All the 29 monoclonal antibodies could be distinguished, on the basis of differential reactivities to various tumors and normal human tissues, from several monoclonal antibodies (B1.1, B6.2, B72.3) reactive with colon carcinomas that have been previously generated in our laboratory. Further immunohistochemical and radiolocalization studies will further define the potential clinical utility of the monoclonals described.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Heryln, M., Steplewsky, Z., Herlyn, D. and H. Koprowsky, 1973, Colorectal carcinoma - specific antigen: Detection by means of monoclonal antibodies, Proc. Natl. Sci. USA 76: 1438–1442.
Koprowsky, H., Steplewsky, Z., Mitchel, K., Herlyn, M., Herlyn, D. and P. Fuhrer, 1979, Colorectal carcinoma antigens detected by hybridoma antibodies, Somatic Cell Genet. 5: 957–971.
Sell, S., 1978, in Water, H. (Ed.): The Handbook of Cancer Immunology, vol. 3, New York, Garland STP M, p.l.
Lewis, M.G., Phillips, T.M., Rowden, G., 1978, in Water, H. (Ed.): The Handbook of Cancer Immunology, vol. 3, New York, Garland STP M, p. 159.
Ferrone, S., Pellegrino, M.A., 1978, in Walter H. (Ed.): The Handbook of Cancer Immunology, vol. 3, New York, Garland STP M, p. 291.
Leibovitz, A., Stinson, J.C., McCombs, W.B., McCoy, C.E., Masur, C. and Malony, N.D., 1976, Classification of human colorectal adenocarcinoma cell lines, Cancer Res. 36: 4562–4563.
Brockhaus, M., Magnani, J.L., Blaszczy, K.M., Steplousky, Z., Kopvowski, H., Karlsson, K.A., Larson, G. and V. Ginsburg, 1981, Monoclonal antibody directed against the human Leb blood group antigen, J. Biol. Chem. 256: 13223–13225.
Colcher, D., Hand, P., Nuti, M. and J. Schlom, 1981, A spectrum of monoclonal antibodies reactive with human mammary tumor cells. Proc. Natl. Acad. Sci. USA 78: 3199–3203.
Colcher, D., Hand, P., Nuti, M. and J. Schlom, 1983, Differential binding to human mammary and non-mammary tumors of monoclonal antibodies reactive with carcinoembryonic antigen, Cancer Invest. 1: 127–138.
Stramignani, D., Bowen, R., Atkinson, B. and J. Schlom, 1983, Differential reactivity of monoclonal antibodies with human colon adenocarcinomas and adenomas, Int. J. Cancer 31: 543–552.
Krupey, J.T., Wilson, S., Freedman, O. and P. Gold, 1972, The preparation of purified carcinoembryonic antigen of the human digestive system from large quantities of tumor tissues, Immunochemistry 9:617-
Coligan, J.E., Lautenschleger, J.T., Egan, M.L. and C.W. Todd, 1972, Isolation and characterization of carcinoembryonic antigen, Immunochemistry 9:377-
Goldenberg, D.M., Sharkey, R.M. and J. Primus, 1976, Carcinoembryonic antigen in histopathology: immunoperoxidase staining of conventional tissue section, JNCI 57: 11–22.
von Kleist, S., Charanel, G. and P. Butin, 1972, Identification of an antigen from normal human tissue that cross reacts with CEA, Proc. Natl. Acad. Sci. USA 9: 2492–2501.
Mach, J.P. and G. Pusztaszeri, 1972, CEA: demonstration of a partial identity between CEA and a normal glycoprotein, Immunochemistry 9: 1031–1038.
Burtin, P., Quan, P.C. and M.C. Sabine, 1975, Non specific cross reacting antigen as a marker for human polymorphs, macrophages and monocytes, Nature 255: 714–716.
Ahnen, D.J., Nokane, P.K. and W.R. Brown, 1982, Vetro-structural localization of carcinoembryonic antigen in normal intestine and colon cancer, Cancer 49: 2077–2090.
O’Brien, M.J., Zamcheck, N., Burke, B., Kirkham, S.E., Saravis, C.A. and L.S. Gottlieb, 1981, Immunocytochemical localization of CEA in benign and malignant colonrectal tissues, Amer. J. Clin. Path. 75: 283–290.
Lowry, O.H., Rosenbrough, N.J. and A.L. Farr, 1951, Protein measurement with the Folin phenol reagent, J. Biol. Chem. 193: 265–275.
Herzenberg, L.A., Herzenberg, L.A. and C. Milstein, 1978, Cell hybrids of myelomas with antibody forming cells and T lymphocytes with T cells, in Weir, D.M. (Ed.): Handbook of Experimental Immunology, London, Blackwell Scientific Publications, pp. 25.1–25.7.
Colcher, D., Hand, P., Teramoto, Y.A., Wunderlich, D. and J. Schlom, 1981, Use of monoclonal antibodies to define the diversity of mammary tumor vital gene products in virions and mammary tumors of the gene Mus, Cancer Res. 41: 1451–59.
Hsu, S.M., Raine, L. and H. Fanger, 1981, Use of avidinbiotinperoxidase complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody ( PA) procedures, J. Histochem. Cytochem. 29: 577–580.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Plenum Press, New York
About this chapter
Cite this chapter
Muraro, R., Wunderlich, D., Schlom, J. (1984). Generation of Monoclonal Antibodies Reactive with Human Colon Carcinomas. In: Aaronson, S.A., Frati, L., Verna, R. (eds) Genetic and Phenotypic Markers of Tumors. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4856-6_12
Download citation
DOI: https://doi.org/10.1007/978-1-4684-4856-6_12
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4858-0
Online ISBN: 978-1-4684-4856-6
eBook Packages: Springer Book Archive