Abstract
The cell surface is the interface between the cell and its environment. The surface acts to regulate cell function through antigens, receptors, cell-cell interaction, and other factors. One important and measurable property of the cell surface is charge. Surface charge results from the accumulation of exposed charge groups at the external surface of the cell membrane, and is peripheral to the region of transmembrane ion separation which defines transmembrane potential. The surface of normal human cells is negatively charged due to the presence of acidic oligosaccharide groups at the terminal regions of membrane glycoproteins (1).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Tenforde, T., Microelectrophoretic studies on the surface chemistry of erythrocytes, Adv. Biol. Med. Physics 13: 43 (1971).
Seaman, G. V. F., Electrokinetic behavior of red cells, in: “The Red Cell,” Vol. II, D. Surgenor, ed., Academic Press, New York (1975), p. 1135.
Pretlow, T. G. and Pretlow, T. P., International Review of Cytology 61: 85 (1979).
Ambrose, E. J., ed., “Cell Electrophoresis,” Churchill Ltd., London (1965).
Vassar, P. S., Hards, J. M., and Seaman, G. V. F., Surface properties of human lymphocytes, Biochim. Biophys. Acta 291: 107 (1973).
Seaman, G. V. F. and Uhlenbruck, G., The surface structure of erythrocytes from some animal sources, Arch. Biochem. Biophys. 100: 493 (1963).
Chollet, P. H., Chassagne, C., Thierry, C., Sauvezie, B., Serrou, B., and Plagne, R., Isolation and electrophoretic mobility of three lymphoid populations in normal human blood, Eur. J. Cancer 13: 333 (1977).
Kapken, J. H. and Uzgiris, Identification of T and B cell sub-populations in human peripheral blood: electrophoretic mobility distributions associated with surface marker characteristics, J. Immunol. 117: 115 (1976).
Wiig, J. N., Electrophoresis of lymphoid cells. A study of Bruton type of agammaglobulinaemia, thymic dysplasia, chronic lymphatic leukaemia and of normal human thymocytes, Clin. Exptl. Immunol. 19: 159 (1975).
Hause, L. L., Koethe, S. M., Rothwell, D. J., and Straumfjord, Jr., Relations between surface charge and in vitro lysis of red blood cells in paroxysmal nocturnal hemoglobinuria, Scand. J. Haematol. 16: 241 (1978).
Field, E. J., Caspary, E. A., and Smith, K. S., Macrophage electrophoretic mobility (MEM) test in cancer: a critical evaluation, Brit. J. Cancer 28: 208 (1973).
Hoffmann, W., Werner, W., Steiner, R., and Kaufmann, R., Cell electrophoresis for diagnostic purposes. I. Diagnostic value of the electrophoretic mobility test (EMT) for the detection of gynaecological malignancies, Brit. J. Cancer 43:
Ruhenstroth-Bauer, G., in: “Cell Electrophoresis,” E. J. Ambrose, ed., Churchill, London, (1965), p. 66.
Zeller, K. and Hannig, K., Free-flow electrophoretic separation of lymphocytes. Evidence for specific organ distributions of lymphoid cells, Hoppe-Seyler’s Z. Physiol. Chem. Bd. 352: 1162 (1971).
Gottschalk, A., Neuraminidase: the specific enzyme of influenza virus and Vibrio cholerae, Biochim. Biophys. Acta 23: 645 (1957).
Eylar, E. H., Madoff, M. A., Brody, 0. V., and Oncley, J. L., The contribution of sialic acid to the surface charge of the erythrocyte, J. Biol. Chem. 237: 1992 (1962).
Lillie, R. S., On differences in the direction of the electrical connection of certain free cells and nuclei, Amer. J. Physiol. viii: 273 (1902).
Valet, G., Bamberger, S., Hofmann, H., Schindler, R., and Ruhenstroth-Bauer, G., Flow cytometry as a new method for the measurement of electrophoretic mobility of erythrocytes using membrane charge staining by fluoresceinated polycations, J. Histochem. Cytochem. 27: 342 (1979).
Hause, L. L., Pattillo, R. A., Sauces, A., Jr., and Mattingly, R. F., Cell surface coatings and membrane potentials of malignant and nonmalignant cells, Science 169: 601 (1970).
Currie, G. A. and Bagshawe, K. D., The masking of antigens on trophoblast and cancer cells, Lancet 1: 708 (1967).
Vassar, P. S., The electric charge density of human tumor cell surfaces, Lab. Invest. 12: 1072 (1963).
Beckmann, A., Jenssen, H. L., Kalkoff, W., and Redmann, K., Transmembrane potential of white cells is found along with some surface potential, Separatum Experientia 26: 186 (1970).
Pattillo, R. A. and Gey, G. 0., The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro, Cancer Res. 28: 1231 (1968).
Hussa, R. 0., Pattillo, R. A., Ruckert, A. C. F., and Scheuermann, K. W., Effects of butyrate and dibutyryl cyclic AMP on hCG-secreting trophoblastic and non-trophoblastic cells, J. Clin. Endocrinol. Metab. 46: 69 (1978).
Kornfeld, R. and Kornfeld, S., The structure of a phytohemagglutinin receptor site from human erythrocytes, J. Biol. Chem. 245: 2536 (1970).
Yorde, D. E., Hussa, R. 0., Garancis, J. C., and Pattillo, R. A., Immunocytochemical localization of human choriogonado-tropin in human malignant trophoblast, Lab. Invest. 40: 391 (1979).
Cole, L. A., Birken, S., Sutphen, S., Hussa, R. 0., and Pattillo, R. A., Absence of the COOH-terminal peptide on ectopic human chorionic gonadotropin 13-subunit (hCGß), Endocrinology 110: 2198 (1982).
Mead, J. S., Horan, P. K., and Wheeless, L. L., Syringing as a method of cell dispersal, Acta Cytologica 22: 86 (1978).
Hause, L. L., Rothwell, D. J., and Straumfjord, Jr., J. V., Measurement of cellular surface charge, Proc. 27th Ann. Conf. Engineer. Biol., Chevy Chase, Maryland (1974), p. 404.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Plenum Press, New York
About this chapter
Cite this chapter
Hause, L.L., Tosaka, N., Pattillo, R.A. (1984). The Surface Charge of Cells Producing hCG. In: Pattillo, R.A., Hussa, R.O. (eds) Human Trophoblast Neoplasms. Advances in Experimental Medicine and Biology, vol 176. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4811-5_16
Download citation
DOI: https://doi.org/10.1007/978-1-4684-4811-5_16
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4813-9
Online ISBN: 978-1-4684-4811-5
eBook Packages: Springer Book Archive