Skip to main content

Use of Autoradiographic Techniques for the Localization of Neurotransmitter Receptors in Brain and Periphery: Recent Applications

  • Chapter
  • First Online:

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 175))

Abstract

The role of the receptor in mediating drug and neurotransmitter action has become increasingly more well-defined. Studies of a compound’s interaction with its receptor has led to the concept of rational drug design and subsequent in vitro testing procedures. Anatomical localization of these drug and neurotransmitter receptors can lead to an increased understanding of their potential role in physiological and behavioral responses, as well as helping to define mechanisms of drug action.

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. S.H. Snyder, Overview of neurotransmitter binding, in: “Neurotransmitter Receptor Binding,” S.J. Enna and M.J. Kuhar, eds., Raven Press, New York, p. 1 (1978).

    Google Scholar 

  2. W.S. Young and M.d. Kuhar, A new method for receptor autoradiography: H-opioid receptor labeling in mounted tissue sections, Brain Res. 179: 255 (1979).

    Article  CAS  Google Scholar 

  3. J.M. Palacios, D.L. Niehoff and M.J. Kuhar, Receptor autoradiography with tritium sensitive film: potential for computerized densitometry, Neurosci. Lett. 25: 101 (1981).

    Article  CAS  Google Scholar 

  4. J.K. Wamsley and J.M. Palacios, Receptor mapping by histochemistry, in: “Handbook of Neurochemistry,” Vol. 2, A. Lajtha, ed., Plenum Press, New York, p. 27 (1982).

    Google Scholar 

  5. J.P. Bennett, Jr., Methods in binding studies, in: “Neurotransmitter Receptor Binding,” H.I. Yamamura, S.J. Enna and M.J. Kuhar, eds., Raven Press, New York, p. 57 (1978).

    Google Scholar 

  6. J.K. Wamsley and J.M. Palacios, Apposition techniques of autoradiography for microscopic receptor localization, in: “Current Methods in Cellular Neurobiology,” J. Barker and J. McKelvy, eds., John Wiley and Sons, New York, p. 241 (1983).

    Google Scholar 

  7. J.R. Unnerstall, D.L. Niehoff, M.J. Kuhar and3J.M. Palacios, Quantitative receptor autoradiography using [H] ultrofilm: Application to multiple benzodiazepine receptors, J. Neurosci. Methods 6: 59 (1982).

    Article  CAS  Google Scholar 

  8. D.R. Gehlert, H.I. Yamamura and J.K Wamsley, Autoradiographic localization of peripheral benzodiazepine blinding sites in the rat brain and kidney using [H1–R05–4864, Eur. J. Pharmacol., in press.

    Google Scholar 

  9. J.K. Wamsley, M.A. Zarbin, N.J.M. Birdsall and M.J. Kuhar, Muscarinic cholinergic receptors: Autoradiographic localization of high and low affinity agonist binding sites, Brain Res. 200: 1 (1980).

    Article  CAS  Google Scholar 

  10. M.A. Zarbin, J.K. Wamsley and M.J. Kuhar, Axonal transport of muscarinic cholinergic receptors in rat vagus nerve: High and low affinity agonist receptors move in opposite directions and differ in nucleotide sensitivity, J. Neurosci. 2: 934 (1982).

    Article  CAS  Google Scholar 

  11. E. Burgisser, A. DeLeon and R.J. Lefkowitz, Reciprocal modulation of agonist and antagonist binding to muscarinic cholinergic receptor by guanine nucleotide, Proc. Natl. Acad. Sci. 79: 1732 (1982).

    Article  ADS  CAS  Google Scholar 

  12. F.J. Ehlert, W.R. Roeske and H.I. Yamamura, Muscarinic receptor: Regulation by guanine nucleotides, ions and N-ethylmaleimide, Fed. Proc. 40: 153 (1981).

    CAS  PubMed  Google Scholar 

  13. N.J.M. Birdsall, A.S.V. Burgen and E.C. Hulme, Correlation between the binding properties and pharmacological responses of muscarinic receptors, in: “Cholinergic Mechanisms and Psychopharmacology,” D.J. Jenden, ed., New York, Plenum Press, p. 25 (1977).

    Google Scholar 

  14. N.J.M. Birdsall and E.C. Hulme, Biochemical studies on muscarinic acetylcholine receptors, J. Neurochem. 27: 7 (1976).

    Article  CAS  Google Scholar 

  15. P.G. Strange, N.J.M. Birdsall and A.S.V. Burgen, Occupancy of muscarinic acetylcholine receptors stimulates a guanylate cyclase in neuroblastoma cells, Biochem. Soc. Trans. 5: 189 (1977).

    Article  CAS  Google Scholar 

  16. J.K. Wamsley, M.A. Zarbin and M.J. Kuhar, Muscarinic cholinergic receptors flow in the sciatic nerve, Brain Res. 217: 155 (1981).

    Article  CAS  Google Scholar 

  17. R. Hammer, C.P. Berrie, N.J.M. Birdsall, A.S.V. Burgen and E.C. Hulme, Pirenzepine distinguishes between different subclasses of muscarinic receptors, Nature 283: 90 (1980).

    Article  ADS  CAS  Google Scholar 

  18. N.J.M. Birdsall, A.S.V. Burgen, R. Hammer, E.C. Hulme and J. Stockton, Pirenzepine - a ligand with original binding properties to muscarinic receptors, Scand. J. Gastroenterol. 15, Suppl. 66: 1 (1980).

    CAS  Google Scholar 

  19. M. Watson, W.R. Roeske and H.I. Yamamura, [3H]-Pirenzepine selectively identifies a high affinity population of muscarinic cholinergic receptors in the rat cerebral cortex, Life Sci. 31: 2019 (1982).

    CAS  Google Scholar 

  20. M. Watson, H.I. Yamamura and W.R. Roeske, A unique re3gulatory profile and regional distribution of [H]-pirenzepine in the rat provide evidence for distinct M1 and M2 muscarinic receptor subtypes, Life Sci. 32: 3001 (1983).

    Article  CAS  Google Scholar 

  21. J.K. Wamsley, M. Lewis, W.S. Young, III and M.J. Kuhar, Autoradiographic localization of muscarinic cholinergic receptors in the rat brainstem, J. Neurosci. 1: 176 (1981).

    Article  CAS  Google Scholar 

  22. H.I. Yamamura, J.K. Wamsley, P. Deshmukh and W.R. Roeske, Differential light microscopic autoradiographic localization of muscarinic cholinergic rece.gtors in the brainstem and spinal cord of the rat using [H]-pirenzepine, Eur. J. Pharmacol. 91: 147 (1983).

    Article  CAS  Google Scholar 

  23. J.K. Wamsley, D.R. Gehlert, W.R. Roeske and H.I. Yamamura, Muscarinic antagonist binding site heterogeneity as e3idenced by atoradiography after direct labeling with [H]-QNB and [H]-pirenzepine, Life Sci., in press.

    Google Scholar 

  24. E. Costa and A. Guidotti, Molecular mechanisms in the receptor action of benzodiazepines, Ann. Rev. Pharmacol. Toxicol. 19: 531 (1979).

    Article  CAS  Google Scholar 

  25. W. Haefely, P. Polc, R. Schaffner, H.H. Keller, L. Pieri and H. Móhler, Facilitation of GABA-ergic transmission of drugs, in: “GABA-Neurotransmitters,” P. Krogsgaard-Larsen, J. Scheel-Kruger and H. Kofod, eds., Munksgaard, Copenhagen, p. 357 (1979).

    Google Scholar 

  26. W. Haefely, L. Pieri, P. Pole and R. Schaffner, in: “Handbook of Experimental Pharmacology,” F. Hoffmeister and G. Stille, eds., Springer-Verlag, Berlin, p. 213 (1981).

    Google Scholar 

  27. R.W. Olsen, GABA-benzodiazepine-barbiturate receptor interactions, J. Neurochem. 37: 1 (1981).

    Article  CAS  Google Scholar 

  28. R.W. Olsen, Drug interactions at the GABA receptor ionophore complex, Ann. Rev. Pharmacol. Toxicol. 22: 245 (1982).

    Article  CAS  Google Scholar 

  29. K.W. Gee, J.K. Wamsley and H.I. Yamamura, Light microscopic autoradiographic identification of picrotoxin/barbiturate b7ipgding sites in rat brain with [S]-t-butyl-bicyclophosphorothionate, Eur. J. Pharmacol. 89: 323 (1983).

    Article  CAS  Google Scholar 

  30. R. Squires, J.E. Casida, M. Richardson and E. Saederup, [S]-t-butylbicyclophosphorothionate binds with high affinity to brain specific sites coupled to GABA-A and ion recognition sites, Mol. Pharmacol. 23: 326 (1983).

    CAS  PubMed  Google Scholar 

  31. W.S. Young and M.J. Kuhar, Autoradiographie localization of benzodiazepine receptors in the brains of humans and animals, Nature 280: 393 (1979).

    Article  ADS  Google Scholar 

  32. W.S. Young and M.J. Kuhar, Radiohistochemical localization of benzodiazepine receptors in rat brain, J. Pharmacol. Exp. Therap. 212: 337 (1980).

    CAS  Google Scholar 

  33. J.K. Wamsley, K.W. Gee and H.I. Yamamura, Comparison of the distribution of convulsant/barbiturate and benzodiazepine receptors using light microscopic autoradiography, Life Sci. 33: 2321 (1983).

    Article  CAS  Google Scholar 

  34. R.W. Olsen and F. Leeb-Lundberg, Convulsant and anticonvulsant drug binding sites related to the GABA receptor/ionophore system, in: “Neurotransmitters, Seizures and Epilepsy,” P.O. Morselli, K.G. Lloyd, W. Lgscher, B.S. Meldrum and E.H. Reynolds, eds., Raven Press, New York, p. 151 (1981).

    Google Scholar 

  35. F. Leeb-Lundberg and R.W. Olsen, Heterogeneity of benzodiazepine receptor interactions with gamma-aminobutyric acid and barbiturate receptor sites, Mol. Pharmacol. 23: 315 (1983).

    CAS  PubMed  Google Scholar 

  36. R. Hammer and F.W. Koss, The pharmacokinetic profile of pirenzepine, Scand. J. Gastroenterol. 14, Suppl. 57: 1 (1979).

    CAS  Google Scholar 

  37. H.I. Yamamura, K.W. Gee, R.E. Brinton, J.P. Davis, M. Hadley and J.K. Wamsley, Tight microscopic autoradiographic visualization of [H]-arginine vasopressin binding sites in the rat brain, Life Sci. 32: 1919 (1983).

    Article  CAS  Google Scholar 

  38. D.G. Baskin, F. Petracca and D.M. Dorsa, Autsradiogrgphic localization of specific binding sites for [H]-[Arg] vasopressin in the septum of the rat brain with tritium sensitive film, Eur. J. Pharmacol. 90: 155 (1983).

    Article  CAS  Google Scholar 

  39. F.W. Van Leeuwen and P. Wolters, Lyht microscopic autoradiographic localization of [H]-arginine-vasopressin binding sites in the rat brain and kidney, Neurosci. Lett. 41: 61 (1983).

    Article  Google Scholar 

  40. D.R. Gehlert, R.C. Speth, D.P. Healy and J.K. Wamsley, Autoradiographic localization of angiotensin II receptors in the rat brainstem, Life Sci., in press.

    Google Scholar 

  41. R. Quirion, C.W. Shul.ts, T.W. Moody, C.B. Pert, T. N. Chase and T.L. O’Donohue, Autoradiographic distribution of substance P receptors in rat central nervous system, Nature 203: 714 (1983).

    Article  ADS  CAS  Google Scholar 

  42. C.J. Heike, J.J. Neil, V.J. Massari and A.D. Loewy, Substance P neurons project from the ventral medulla to the intermediolateral cell column and ventral horn in the rat, Brain Res. 243: 147 (1982).

    Article  Google Scholar 

  43. J.K. Wamsley, Muscarinic cholinergic receptors undergo axonal transport in the brain, Eur. J. Pharmacol. 86: 309 (1983).

    Article  Google Scholar 

  44. E. Snowhill and J.K. Wamsley, Serotonin type-2 receptors undergo axonal transport in the medial forebrain bundle, Eur. J. Pharmacol., in press.

    Google Scholar 

  45. C.U. Vorhees, D.E. Schmidt and R.J. Barrett, Effects of pyrithiamin and oxythiamin on acetylcholine levels and utilization in rat brain, Brain Res. Bull. 3: 493 (1978).

    Article  CAS  Google Scholar 

  46. K.V. Speeg, D. Chen, D.W. McCandless and S. Scheuker, Cerebral acetylcholine in thiamine deficiency, Proc. Soc. Exp. Biol. Med. 135: 1005 (1970).

    Article  Google Scholar 

  47. G.A. Bray and D.A. York, Hypothalamic and genetic obesity in experimental animals: An autonomic and endocrine hypothesis, Physiol. Rev. 59 (3): 719 (1979).

    Article  CAS  Google Scholar 

  48. S.C. Woods and D. Porte, The central nervous system, pancreatic hormones, feeding and obesity, Adv. Metab. Disord. 9: 283 (1978).

    Article  CAS  Google Scholar 

  49. H. Shoemaker, R.G. Boles, W.D. Horst and H31. Yamamura, Specific high affinity binding sites for [311]-R05–4864 in rat brain and kidney, J. Pharmacol. Exp. Therap. 225: 61 (1983).

    Google Scholar 

  50. P.J. Whitehouse, J.K. Wamsley, M.A. Zarbin, D.L. Price, W.W. Tourtellotte and M.J. Kuhar, Amyotrophic lateral sclerosis: Alteration in neurotransmitter receptors, Ann. Neurol. 14: 8 (1983).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1984 Plenum Press, New York

About this chapter

Cite this chapter

Gehlert, D.R., Yamamura, H.I., Wamsley, J.K. (1984). Use of Autoradiographic Techniques for the Localization of Neurotransmitter Receptors in Brain and Periphery: Recent Applications. In: Kito, S., Segawa, T., Kuriyama, K., Yamamura, H.I., Olsen, R.W. (eds) Neurotransmitter Receptors. Advances in Experimental Medicine and Biology, vol 175. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-4805-4_20

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-4805-4_20

  • Published:

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4684-4807-8

  • Online ISBN: 978-1-4684-4805-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics