Metabolism of Food Toxicants: Saccharin and Aflatoxin B1, A Contrast in Metabolism and Toxicity

  • J. L. Byard
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 177)


The artificial sweetener, saccharin, and the secondary mold product, aflatoxin B1, are present in many foods consumed by humans. Both chemicals produce cancer in rats. For this reason, they have aroused concern among scientists and the public as to the carcinogenic risk that they pose to humans. A comparison of these two chemicals reveals striking contrasts in potency, metabolism, mechanism-of-action, and experimental approach to assessing metabolism. These contrasts are examined in detail to illustrate the importance of metabolism in safety evaluation.


Covalent Binding Aspergillus Parasiticus Cyclohexene Oxide Primary Hepatocyte Culture Diethyl Maleate 
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  1. Arnold, D.L., C.A. Moodie, H.C. Grice, S.M. Charbonneau, B. Stauric, B.T. Collins, P.F. McGuire, Z.Z. Zawidzka and I.C. Munro, 1980, Long-term toxicity of ortho-toluenesulfonamide and sodium saccharin in the rat, Toxicol. Appl. Pharmacol., 52: 113.Google Scholar
  2. Booth, S.C., H. Bosenberg, R.C. Garner, P.J. Hertzog and K. Norpoth, 1981, The activation of aflatoxin B1 in liver slices and in bacterial mutagenicity assays using livers from different species including man, Carcinogenesis, 2: 1063.PubMedCrossRefGoogle Scholar
  3. Byard, J.L., E.W. McChesney, L. Golberg and F. Coulston, 1974, Excretion and metabolism of saccharin in man: II. Studies with [14C] labeled and unlabeled saccharin, Fd. Cosmet. Toxicol., 12: 175.Google Scholar
  4. Byard, J.L. and L. Golberg, 1973, Metabolism of saccharin in laboratory animals, Fd. Cosmet. Toxicol., 11: 391.Google Scholar
  5. Cranmer, M.F., 1980, “Saccharin, a Report,” American Drug Research Institute, U.S.A.Google Scholar
  6. Croy, R.G. and G.N. Wogan, 1981, Quantitative comparison of covalent aflatoxin-DNA adducts formed in rat and mouse livers and kidneys, J. Natl. Cancer Inst., 66: 761.Google Scholar
  7. Decad, G.M., K.K. Dougherty, D.P.H. Hsieh and J.L. Byard, 1979, Metabolism of aflatoxin Bj in cultured mouse hepatocytes: Comparison with rat and effects of cyclohexene oxide and diethyl maleate, Toxicol, Appl. Pharmacol,, 50: 429.Google Scholar
  8. Essigman, J.M., R.G. Croy, A.M. Nadzan, W.F. Busby, V.N. Reinhold, G. Buchi and G.N. Wogan, 1977, Structural identification of the major DNA adduct formed by aflatoxin B1 in vitro, Proc. Natl. Acad. Sei. U.S.A., 74: 1870.Google Scholar
  9. Heathcote, J.G. and J.R. Hibbert, 1978, “Aflatoxins: Chemical and Biological Aspects,” Elsevier, New York.Google Scholar
  10. Lin, J., J.A. Miller and E.C. Miller, 1977, 2,3-Dihydro-3-(guan-7-yl)-3-hydroxy-aflatoxin B1, a major acid hydrolysis product of aflatoxin B1-DNA of ribosomal RNA adducts formed in hepatic microsome-mediated reactions and rat liver in vivo, Cancer. Res., 37: 4430.Google Scholar
  11. Lutz, W.K., 1979, In vivo covalent binding of organic chemicals to DNA as a quantitative indicator in the process of chemical carcinogenesis, Mutation Research, 65: 289.PubMedCrossRefGoogle Scholar
  12. Lutz, W.K. and C.H. Schlatter, 1977, Saccharin does not bind to DNA of liver or bladder in the rat, Chem.-Biol. Interactions, 19: 253.Google Scholar
  13. Mathews, H.B., M. Fields and L. Fishbein, 1973, Saccharin Distribution and excretion of a limited dose in the rat, J. Agr. Food Chem., 21: 916.Google Scholar
  14. McCann, J., E. Choi, E. Yamasaki and B.N. Ames, 1975, Detection of carcinogens as mutagens in the Salmonella/microsome test: Assay of 300 chemicals, Proc. Nat. Acad. Sei. USA, 72: 5135.Google Scholar
  15. Minegishi, K.I., M. Asahina and T. Yamaha, 1972, The metabolism of saccharin and related compounds in rats and guinea pigs, Chem. Pharm. Bull., 20: 1351.Google Scholar
  16. Sweatman, T.W., A.G. Renwick and C.D. Burgess, 1981, The pharmacokinetics of saccharin in man, Xenobiotica, 11: 531.PubMedCrossRefGoogle Scholar
  17. Wogan, G.N., 1973, Aflatoxin carcinogenesis, Methods Cancer Res., 7: 309.Google Scholar
  18. Wogan, G.N., S. Paglialunga and P.M. Newberne, 1974, Carcinogenic effects of low dietary levels of aflatoxin B1 in rats, Fd. Cosmet. Toxicol., 12: 681.Google Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • J. L. Byard
    • 1
  1. 1.Department of Environmental ToxicologyUniversity of CaliforniaDavisUSA

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