Abstract
Growth of the yeast Rhodotorula graminis on benzoate results in the induction of benzoate hydroxylase, p-hydroxybenzoate-3-hydroxy-lase, protocatechuate-3,4-dioxygenase, and enzymes of the ß-ketoadi-pate pathway. Following subcellular fractionation of crude extracts of benzoate-grown cells, benzoate hydroxylase activity was observed in the particulate (membrane) fraction. Thus, washed membrane preparations, which are devoid of other benzoate-dissimilatory enzymes, were used as a source of partially purified benzoate hydroxylase for further study.
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© 1984 Plenum Press, New York
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McNamee, C., Durham, D.R. (1984). Membrane-Associated Benzoate-4-Hydroxylase from Rhodotorula Graminis. In: Omenn, G.S., et al. Genetic Control of Environmental Pollutants. Basic Life Sciences, vol 28. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4715-6_26
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DOI: https://doi.org/10.1007/978-1-4684-4715-6_26
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4717-0
Online ISBN: 978-1-4684-4715-6
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