Abstract
Since cerebral metabolic processes are mediated and controlled by enzymes and regulating peptides such as neurohormones, there is considerable interest to relate altered synthesis rates of single specific proteins to the functional activity in various cerebral structures, especially in altered physiological states. The basic mechanisms underlying changes in protein synthesis can be analyzed on a regional level by investigation of the total cellular protein biosynthesis in vivo by radioactive amino acid incorporation2. Coronal sections subjected to autoradiography for regional visualization of in vivo labeled proteins may be further utilized for demonstration of single specific proteins by direct localization of their immunoreactive sites with radioactively labeled antibodies. A correlation can also be carried out with regional blood flow and the energy state of the brain, using appropriate autoradiograph-ic10,9, bioluminescent6,8 and fluoroscopic11 techniques.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Baker RN, Cancilla PA, Pollock PS, Frommes SP: The movement of exogenous protein in experimental edema. An electronmicroscopic study after freeze injury. J Neuropathol Exp Neurol 30: 668–679 (1971).
Bodsch W, Hossmann KA: Regional protein and RNA synthesis in rat brain. Autoradiography and quantitative HPLC. Neurosci Lett Suppl 7: 431 (1981).
Bodsch W, Hossmann KA: A quantitative regional analysis of amino acids involved in rat brain protein synthesis by high performance liquid chromatography. J Neurochem, in press (1982).
Bodsch W, Hürter T, Hossmann KA: Immunochemical method for quantitative evaluation of vasogenic brain edema following cold injury of the rat brain. Brain Res, in press (1982).
Klatzo I, Chui E, Fujiwara K, Spatz M: Resolution of vasogenic brain edema. Adv Neurol 28: 359–373 (1980).
Kogure K, Alonso OF: A pictorial representation of endogenous brain aTP by a bioluminescent method. Brain Res 154: 273–284 (1978).
Mies G, Niebuhr I, Hossmann KA: Simultaneous measurement of blood flow and glucose metabolism by autoradiographic techniques. Stroke 12: 581–588 (1981).
Paschen W, Niebuhr I, Hossmann KA: A bioluminescence method for the demonstration of regional glucose distribution in brain slices. J Neurochem 36: 513–517 (1981).
Sakurada O, Kennedy C, Jehle J, Brown JD, Carbin GL, Sokoloff L: Measurement of local cerebral blood flow with iodo (14C) antipyrine. Am J Physiol 234: H59–66 (1978).
Sokoloff L, Reivich M, Kennedy C, Des Rosiers MH, Patlak CS, Pettigrew KD, Sakurada O, Shinohara M: The (14C) deoxyglucose method for the measurement of local cerebral glucose utilization: Theory, procedure, and normal values in the conscious and anesthetized albino rat. J Neurochem 28: 897–916 (1977).
Welsh FA, Rieder W: Evaluation of in situ freezing of cat brain by NADH fluorescence. J Neurochem 31: 299–309 (1978).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Plenum Press, New York
About this chapter
Cite this chapter
Bodsch, W., Mies, G., Paschen, W., Hossmann, KA. (1984). Regional Quantitative Biochemistry and Autoradiography of Protein Synthesis and Serum Extravasation in Brain Edema. In: Go, K.G., Baethmann, A. (eds) Recent Progress in the Study and Therapy of Brain Edema. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4616-6_3
Download citation
DOI: https://doi.org/10.1007/978-1-4684-4616-6_3
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4618-0
Online ISBN: 978-1-4684-4616-6
eBook Packages: Springer Book Archive