Abstract
Essential to an understanding of membrane-related biological phenomena at the molecular level is not only identification of the components involved but rather knowledge of their respective structural and functional characteristics. Such knowledge is attained by disintegrating biological membranes into particulate entities whose chemical properties can then be more specifically investigated. Successful reconstitution of isolated components in model systems leads to an improved understanding of transmembrane processes. Membrane architecture and the mechanism of transbilayer events are, however, most conclusively explored within a functionally active membrane. The latter approach therefore requires selective identification of defined membrane components in situ. As a consequence, the search for means to locate and specifically characterize defined membrane constituents has intentionally been pursued. This objective is most efficiently achieved by the use of chemical modification. In spite of inherent limitations, the approach offers obvious advantages in that (1) the extent and topology of label binding can be controlled chemically, and (2) label allocation is feasible at both the membrane and molecular level.
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Sigrist, H., Zahler, P. (1985). Selective Covalent Modification of Membrane Components. In: Martonosi, A.N. (eds) The Enzymes of Biological Membranes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4598-5_10
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