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Quantitative Mass Spectrometry and Its Application in Microbiology

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Book cover Gas Chromatography Mass Spectrometry Applications in Microbiology

Abstract

Advanced chemoanalytical techniques are now becoming introduced in microbiological laboratories, thereby adding new dimensions to traditional methods of studying microbial metabolic processes.

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References

  • Arpino, P. J., Baldwin, M. A., and McLafferty, F. W., 1974, Liquid chromatography-mass spectrometry. II. Continuous monitoring, Biomed. Mass Spectrom. 1:80.

    Article  PubMed  CAS  Google Scholar 

  • Arpino, P. J., Krien, P., Vajta, S., and Devant, G., 1981, Optimization of the instrumental parameters of a combined liquid chromatograph-mass spectrometer, coupled by an interface for direct introduction. II. Nebulization of liquids by diaphragms. J. Chromatogr. 203:117.

    Article  CAS  Google Scholar 

  • Bengtsson, G., and Mårdh, P. A., 1978, The application of gas chromatography-mass spectrometry for detection of free amino acids in cultures of Mycoplasmatales and in tissue cell cultures contaminated by mycoplasmas, p. 127, Abstracts of the XIIth Int. Congress of Microbiology ,Munich, West Germany.

    Google Scholar 

  • Bengtsson, G., and Odharn, G., 1979, A micromethod for the analysis of free amino acids by gas chromatography and its application to biological systems, Anal. Biochem. 92:426.

    Article  PubMed  CAS  Google Scholar 

  • Bengtsson, G., Odham, G., and Westerdahl, G., 1981, Glass capillary gas Chromatographic analysis of free amino acids in biological microenvironments using electron capture or selected ion-monitoring detection, Anal. Biochem. 111:163.

    Article  PubMed  CAS  Google Scholar 

  • Bertilsson, L., and Costa, E. ,1976, Mass fragmentographic quantitation of glutamic acid and y-aminobutyric acid in cerebellar nuclei and sympathetic ganglia of rats, J. Chromatogr. 118:395.

    Article  PubMed  CAS  Google Scholar 

  • Coutts, R. T., Jones, G. R., and Liu, S. F. ,1979, Quantitative gas chromatography-mass spectrometry of trace amounts of glutamic acid in water samples, J. Chromatogr. Sci. 17:551.

    CAS  Google Scholar 

  • deLeenheer, A. D., and Cruyl, A. A., 1980, “Quantitative Mass Spectrometry,” in Biochemical Applications of Mass Spectrometry first supplementary volume (G. R. Waller, and O. C. Dermer, eds.), p. 1169, John Wiley and Sons, New York.

    Google Scholar 

  • Dutton, G. G. S., 1973, Application of gas-liquid chromatography to carbohydrates, Adv. Carbohyd. Chem. Biochem. 28:11.

    Article  CAS  Google Scholar 

  • Dutton, G. G. S., 1974, Application of gas-liquid chromatography to carbohydrates. II, Adv. Carbohyd. Chem. Biochem. 30:10.

    Google Scholar 

  • Erdahl, W. L., and Privett, O. S., 1977, A new system for lipid analysis by liquid chromatography-mass spectrometry, Lipids 22:797.

    Article  Google Scholar 

  • Falkner, F. C., Sweetman, B. J., and Watson, J. T., 1975, Biomedical applications of selected ion monitoring, Appl. Spectrosc. Rev. 10:51.

    Article  CAS  Google Scholar 

  • Fischer-Hoch, S., Hudson, M. J., and Thompson, M. H., 1979, Identification of a clinical isolate as Legionella pneumophila by gas chromatography and mass spectrometry of cellular fatty acids, Lancet 8138:323.

    Article  Google Scholar 

  • Fox, A., Schwab, J. H., and Cochran, T., 1980, Muramic acid detection in mammalian tissues by gas liquid chromatography-mass spectrometry, Infection and Immunity 29:526.

    PubMed  CAS  Google Scholar 

  • Hammar, C. G., Holmstedt, B., and Ryhage, R., 1968, Mass fragmentography. Identification of chloropromazine and its metabolites in human blood by a new method, Anal. Biochem. 25:532.

    Article  PubMed  CAS  Google Scholar 

  • Hintze, U., Röper, H., and Gercken, G., 1963, Gas chromatography-mass spectrometry of Q-C20 fatty acid benzyl esters. J. Chromatog. 87:481.

    Article  Google Scholar 

  • Kaiser, F. E., Gehrke, C. W., Zumwalt, R. W., and Kuo, K. C., 1974, Amino acid analysis. Hydrolysis, ion-exchange clean up, derivatization, and quantitation by gas-liquid chromatography, J. Chromatog. 94:113.

    Article  CAS  Google Scholar 

  • Klem, H. P., Hintze, U., and Gercken, G., 1973, Quantitative preparation and gas chromatography of short and medium chain fatty acid benzyl esters (C1-C22), J Chromatog. 75:19.

    Article  Google Scholar 

  • Kraska, B., Klemer, A., and Hagedorn, H., 1974, Umsetzung von Kohlenhydraten mit tert-Butylchlordimethylsilan, Carbohyd. Res. 36:398.

    Article  CAS  Google Scholar 

  • Kuksis, A., Myher, J. J., Marai, L., and Geher, K., 1976, Estimation of plasma free fatty acids as the trimethylsilyl (TMS) esters, Anal. Biochem. 70:302.

    Article  PubMed  CAS  Google Scholar 

  • Larsson, L., Mårdh, P. A., Odham, G., and Westerdahl, G., 1980, Detection of tuberculostearic acid in biological specimens by means of glass capillary gas chromatographyelectron and chemical ionization mass spectrometry, utilizing selected ion monitoring, J. Chromatog. Biomed. Appl. 182:402.

    Article  CAS  Google Scholar 

  • Larsson, L., Mårdh, P. A., Odham, G., and Westerdahl, G., 1981, Use of selected ion monitoring for detection of tuberculostearic and C32-mycocerosic acid in mycobacteria and in five-day-old cultures of sputum specimens from patients with pulmonary tuberculosis, Acta Pathol. Microbiol. Scand., Sect. B. 89:245.

    CAS  Google Scholar 

  • Leimer, K. R., Rise, R. H., and Gehrke, C. W., 1977, Complete mass spectra of N- trifluoroacetyl-nbutyl esters of amino acids, J. Chromatog. 141:121.

    Article  CAS  Google Scholar 

  • McFadden, W. H., Bradford, D. C., Games, D. E., and Gower, J. L., 1977, Applications of combined liquid chromatography-mass spectrometry, Am. Lab. October 55.

    Google Scholar 

  • McLafferty, F. W., and Baldwin, M. A., 1976, Liquid chromatography-mass spectrometry system and method. US Pat. 3,997,298.

    Google Scholar 

  • Maitra, S. K., Scholz, M. C., Yoshikawa, T. T., and Guze, L. B., 1978, Determination of lipid A and endotoxin in serum by mass spectroscopy, Proc. Natl. Acad. Sci. USA 75: 3993.

    Article  PubMed  CAS  Google Scholar 

  • March, J. F., 1975, A modified technique for the quantitative analysis of amino acids by gas chromatography using heptafluorobutyric n-propyl derivatives, Anal. Biochem. 69:420.

    Article  PubMed  CAS  Google Scholar 

  • Mårdh, P.-A., Larsson, L., Odham, G., and Engbaek, H. C., 1983, Tuberculostearic acid as a diagnostic marker in tuberculous meningitis, Lancet 1:367.

    Article  PubMed  Google Scholar 

  • Markey, S. P., 1974, in Applications of Gas Chromatography-Mass Spectrometry to the Investigation of Human Disease (O. A. Mamer, W. J. Mitchell, and C. R. Scriver, eds.), p. 239, McGill University, Montreal Childrens Hospital Research Institute, Montreal.

    Google Scholar 

  • Matthews, D. E., Ben-Galim, E., and Bier, D. M., 1979, Determination of stable isotopic enrichment in individual plasma amino acids by chemical ionization mass spectrometry, Anal. Chem. 51:80.

    Article  PubMed  CAS  Google Scholar 

  • Mee, J. M. L., Korth, J., and Halpern, B., 1976, Rapid and quantitative blood analysis for free fatty acids by chemical ionization mass spectrometry, Anal. Lett. 9:1075.

    Article  CAS  Google Scholar 

  • Millard, B. J., 1979, Quantitative Mass Spectrometry ,Heyden, London.

    Google Scholar 

  • Odham, G., Larsson, L., and Mårdh, P. A., 1979, Demonstration of tuberculostearic acid in sputum from patients with pulmonary tuberculosis by selected ion monitoring, J. Clin. Invest. 63:813.

    Article  PubMed  CAS  Google Scholar 

  • Petty, F., Tucker, H. N., Molinary, S. V., Flynn, M. W., and Wanter, J. D., 1976, Quantitation of glycine in plasma and urine by chemical ionization mass fragmentography, Clin. Chim. Acta 66:111.

    Article  PubMed  CAS  Google Scholar 

  • Phillipou, G., Bigham, D. A., and Seamark, R. F., 1975, Subnanogram detection of t- butyldimethylsilyl fatty acid esters by mass fragmentography, Lipids 10:714.

    Article  PubMed  CAS  Google Scholar 

  • Radford, T., and deJongh, D. C., 1980, “Carbohydrates,” in Biochemical Applications of Mass Spectrometry (G. R. Waller, and O. C. Dermer, eds.), p. 255, John Wiley and Sons, New York.

    Google Scholar 

  • Roboz, J., Suzuki, R., and Holland, J. F., 1980, Quantification of arabinitol in serum by selected ion monitoring as a diagnostic technique in invasive Candidiasis, J. Clin. Microbiol. 12:594.

    PubMed  CAS  Google Scholar 

  • Scott, R. P. W., Scott, G. G., Munroe, M., and Hess, J., Jr., 1974, Interface for on-line liquid chromatography-mass spectrometry analysis, J. Chromatog. 99:395.

    Article  CAS  Google Scholar 

  • Sjöqvist, B., and Änggård, E., 1972, Gas Chromatographic determination of homovanillic acid in human cerebrospinal fluid by electron capture detection and by mass fragmentography with a deuterated internal standard, Anal. Chem. 44:2297.

    Article  Google Scholar 

  • Solovyov, A. A., Kadentsev, V. I., and Chizhov, O. S., 1976, Izv. Akad. Nauk. Arm. SSR, Khim. Nauki ,2256; Chem Abstr. 86, 106974 m (1977).

    Google Scholar 

  • Sweeley, C. C., Elliott, W. H., Fries, I., and Ryhage, R., 1966, Mass spectrometric determination of unresolved components in gas Chromatographic effluents, Anal. Chem. 38:1549.

    Article  PubMed  CAS  Google Scholar 

  • Tunlid, A., and Odham, G., 1983, Capillary gas chromatography using electron capture or selected ion monitoring detection for the determination of muramic acid, diaminopimelic acid and the ratio of d/lalanine in bacteria. J. Microbiol. Methods. ,in press.

    Google Scholar 

  • Vetter, W., 1980, “Amino Acids”, in Biochemical Applications of Mass Spectrometry (G. R. Waller, and O. C. Dermer, eds.), p. 439, John Wiley and Sons, New York.

    Google Scholar 

  • Vilkas, M., Jan, H. I., Boussac, G., and Bonnard, M. C., 1966, Chromatographic en phase vapeur de sucres a L’etat de trifluoroacetates, Tetrahedron Lett. 1441.

    Google Scholar 

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Author information

Authors and Affiliations

  1. Laboratory of Ecological Chemistry, University of Lund, Ecology Building, Helgonavägen 5, S-223 62, Lund, Sweden

    Göran Odham

  2. Department of Medical Microbiology, University of Lund, Sölvegatan 23, S-223 62, Lund, Sweden

    Lennart Larsson & Per-Anders Mårdh

Authors
  1. Göran Odham
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  2. Lennart Larsson
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  3. Per-Anders Mårdh
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Editor information

Editors and Affiliations

  1. Laboratory of Ecological Chemistry, University of Lund, Ecology Building, Helgonavägen 5, S-223 62, Lund, Sweden

    Göran Odham

  2. Department of Medical Microbiology, University of Lund, Sölvegatan 23, S-223 62, Lund, Sweden

    Lennart Larsson  & Per-Anders Mårdh  & 

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© 1984 Plenum Press, New York

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Odham, G., Larsson, L., Mårdh, PA. (1984). Quantitative Mass Spectrometry and Its Application in Microbiology. In: Odham, G., Larsson, L., Mårdh, PA. (eds) Gas Chromatography Mass Spectrometry Applications in Microbiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4526-8_10

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  • DOI: https://doi.org/10.1007/978-1-4684-4526-8_10

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-4528-2

  • Online ISBN: 978-1-4684-4526-8

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