Skip to main content
SpringerLink
Log in

Regeneration of Plants from Crown Gall Cells A step in the T-DNA mediated genetic engineering of plants

  • Chapter
Genetic Engineering in Eukaryotes

Part of the book series: NATO Advanced Science Institutes Series ((NSSA,volume 61))

Abstract

The mechanism whereby the plant pathogenic soil bacterium Ag-robacterium tumefaciens induces Crown Gall plant tumors is the result of a remarkable example of naturally occurring genetic engineering. A fragment of about 20 kb (called the T-region) of a 120 kb plasmid (called Ti plasmid for tumor-inducing plasmid) is transferred to the plant cell where it becomes covalently linked to the nuclear DNA (1–7). This integrated DNA has been named the T-DNA. Crown Gall cells have two distinctive properties: They grow without hormones (8,9) and they produce so-called opines: compounds not found in normal plant cells and used specifically by Agrobacterium tumefaciens as a carbon, nitrogen and energy source with the help of a Ti plasmid-encoded uptake and degradation system (10–13). Opines moreover have the capacity to induce a plasmid transfer system which can spread the opine degradation system through an Agrobacterium population (14,15). For recent reviews see 3, 16–20.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Chilton, M.-D., Drummond, H.J., Merlo, D.J., Sciaky, D., Montoya, A.L., Gordon, M.P., and Nester, E.W. (1977) Cell 11: 263

    Article  PubMed  CAS  Google Scholar 

  2. De Beuckeleer, M., De Block, M., De Greve, H., Depicker, A., De Vos, R., De Vos, G., De Wilde, M., Dhaese, P., Dobbelaere, M.R., Engler, G., Genetello, C., Hernalsteens, J. P., Holsters, M., Jacobs, A., Schell, J., Seurinck, J., Silva, B., Van Haute, E., Van Montagu, M., Van Vliet, F., Villarroel, R., and Zaenen, I. (1978) In: Proc. IV. Int. Conf. on Plant Pathogenic Bacteria, INRA, Angers, 115

    Google Scholar 

  3. Schell, J., Van Montagu, M., De Beuckeleer, M., De Block, M., Depicker, A., De Wilde, M., Engler, G., Genetello, C., Hernalsteens, J.P., Holsters, M., Seurinck, J., Silva, B., Van Vliet, F., and Villarroel, R. (1979) Proc. R. Soc. London B 204:251

    Article  CAS  Google Scholar 

  4. Thomashow, M.F., Nutter, R., Montoya, A.L., Gordon, M.P., and Nester, E.W. (1980) Cell 19:729

    Article  PubMed  CAS  Google Scholar 

  5. Lemmers, M., De Beuckeleer, M., Holsters, M., Zambryski, P., Depicker, A., Hernalsteens, J.P., Van Montagu, M. and Schell, J. (1980) J. Mol. Biol. 144:355

    Article  Google Scholar 

  6. Zambryski, P., Holsters, M., Krüger, K., Depicker, A., Schell, J., Van Montagu, M., and Goodman, H.M. (1980) Science 209:1385

    Article  PubMed  CAS  Google Scholar 

  7. De Beuckeleer, M., Lemmers, M., De Vos, G., Willmitzer, L., Van Montagu, M., and Schell, J. (1981) Mol. Gen. Genet. 183:283

    Article  PubMed  Google Scholar 

  8. Braun, A.C. and White, P.R. (1943) Phytopath. 33:85

    CAS  Google Scholar 

  9. Braun, A.C. (1956) Cancer Research 16:53

    PubMed  CAS  Google Scholar 

  10. Petit, A., Delhaye, S., Tempé, J., and Morel, G. (1970) Phy-siol. Vég. 8:205

    CAS  Google Scholar 

  11. Bomhoff, G., Klapwijk, P.M., Kester, C.H.M., Schilperoort, R. A., Hernalsteens, J.P., and Schell, J. (1976) Mol. Gen. Genet. 145:177

    Article  PubMed  CAS  Google Scholar 

  12. Petit, A. and Tempé, J. (1978) Mol. Gen. Genet. 167:147

    Article  CAS  Google Scholar 

  13. Klapwijk, P., Hooykaas, P., Kester, H., Schilperoort, R.A., and Rörsch, A. (1976) J. Gen. Microbiol. 96:155

    PubMed  CAS  Google Scholar 

  14. Kerr, A., Manigault, P., and Tempé, J. (1977) Nature 265:560

    Article  PubMed  CAS  Google Scholar 

  15. Genetello, C., Van Larebeke, N., Holsters, M., Depicker, A., Van Montagu, M., and Schell, J. (1977) Nature 265:561

    Article  PubMed  CAS  Google Scholar 

  16. Gordon, M.P. (1979) In: “Proteins and Nucleic Acids, The Bio-chemistry of Plants”, Vol. 6, A. Marcus ed. Acad. Press, New York, p. 531

    Google Scholar 

  17. Schilperoort, R.A., Klapwijk, P.M., Ooms, G., and Wullems, G.J. (1980) In: Genetic origins of tumor cells. F.J. Cleton and J.W. Simons eds. Martinus Nijhoff, The Hague, p. 87

    Chapter  Google Scholar 

  18. Schell, J. and Van Montagu, M. (1980) In: “Genome Organizati-on and Expression in Plants”. C.J. Leaver ed. Plenum Press, New York, p. 453

    Google Scholar 

  19. Van Montagu, M. and Schell, J. (1979) In: “Plasmids of medical, environmental and commercial importance”. K. Timmis and A. Punier eds. Elsevier, Amsterdam, p.71

    Google Scholar 

  20. Van Montagu, M., Holsters, M., Zambryski, P., Hernalsteens, J.P., Depicker, A., De Beuckeleer, M., Engler, G., Lemmers, M., Willmitzer, L., and Schell, J. (1980) Proc. R. Soc. London B 210:351

    Article  Google Scholar 

  21. Guyon, P., Chilton, M.-D., Petit, A., and Tempé, J. (1980) Proc. Natl. Acad. Sci. USA 77:2693

    Article  PubMed  CAS  Google Scholar 

  22. Drummond, M.H., Gordon, M.P., Nester, E.W., and Chilton, M.-D. (1977) Nature 269:535

    Article  CAS  Google Scholar 

  23. Gurley, W.B., Kemp, J.D., Albert, M.J., Sutton, D.W., and Callis, J. (1979) Proc. Natl. Acad. Sci. USA 76:2828

    Article  PubMed  CAS  Google Scholar 

  24. Willmitzer, L., Otten, L., Simons, G., Schmalenbach, W., Schröder, J., Schröder, G., Van Montagu, M., De Vos, G., and Schell, J. (1981) Mol. Gen. Genet. 182:255

    Article  PubMed  CAS  Google Scholar 

  25. Gelvin, S.B., Gordon, M.P., Nester, E.W., and Aronson, A.J. (1981) Plasmid 6:17

    Article  PubMed  CAS  Google Scholar 

  26. Alwine, J.C., Kemp, D.J., and Stark, G.R. (1977) Proc. Nati. Acad. Sci.USA 74:5350

    Article  CAS  Google Scholar 

  27. Willmitzer, L., Simons, G., and Schell, J. (1982) EMBO J. 1: 139

    PubMed  CAS  Google Scholar 

  28. Willmitzer, L., Dhaese, P., Schreier, P.H., Schmalenbach, W., Van Montagu, M., and Schell, J. (1982) submitted to Cell

    Google Scholar 

  29. Hack, E. and Kemp, J.D. (1980) Pl. Physiol. 65:949

    Article  CAS  Google Scholar 

  30. Kemp, J.D., Sutton, D.W., and Hack, E. (1979) Biochemistry 18:3755

    Article  PubMed  CAS  Google Scholar 

  31. Schröder, J. and Schröder, G. (1982) Mol. Gen. Genet. 185:51

    Google Scholar 

  32. Schröder, J., Hillebrand, A., Klopp, W., and Pühler, A. (1981) Nucl. Acids Res. 9:5187

    Article  PubMed  Google Scholar 

  33. Leemans, J., Deblaere, R., Willmitzer, L., De Greve, H., Her-nalsteens, J.P., Van Montagu, M., and Schell, J. (1982) EMBO J. 1:147

    PubMed  CAS  Google Scholar 

  34. Garfinkel, D.J., Simpson, R.S., Ream, L.W., White, F.F., Gor-don, M.P., and Nester, E.W. (1981) Cell 27:143

    Article  PubMed  CAS  Google Scholar 

  35. Ooms, G., Hooykaas, P., Molenaar, G., Schilperoort, R.A. (1981) Gene 14:33

    Article  PubMed  CAS  Google Scholar 

  36. Hernalsteens, J.P., Van Vliet, F., De Beuckeleer, M., Depick-er, A., Engler, G., Lemmers, M., Holsters, M., Van Montagu, M., and Schell, J. (1980) Nature 187:654

    Article  Google Scholar 

  37. Braun, A. and Wood, H.N. (1976) Proc. Natl. Acad. Sci.USA 73:496

    Article  PubMed  CAS  Google Scholar 

  38. Wood, H.N., Binns, A., and Braun, A.C. (1978) Differentiation 11:175

    Article  CAS  Google Scholar 

  39. Lemmers, M., De Beuckeleer, M., Holsters, M., Zambryski, P., Depicker, A., Hernalsteens, J.P., Van Montagu, M., and Schell, J. (1980) J. Mol. Biol. 144:353

    Article  PubMed  CAS  Google Scholar 

  40. Yang, F., Montoya, A.L., Merlo, D.J., Drummond, M.H., Chilton, M.-D., Nester, E.W., and Gordon, M.P. (1980) Mol. Gen. Genet. 177:707

    Article  PubMed  CAS  Google Scholar 

  41. Yang, F. and Simpson, R.B. (1981) Proc. Natl. Acad. Sci. USA 78:4151

    Article  PubMed  CAS  Google Scholar 

  42. Scott, I.M. (1979) Pl. Sci. Lett. 16:239

    Article  CAS  Google Scholar 

  43. Owens, L.D. (1982) Pl. Physiol. 69:37

    Article  CAS  Google Scholar 

  44. Aerts, M., Jacobs, M., Hernalsteens, J.P., Van Montagu, M. and Schell, J. (1979) Pl. Sci. Lett. 17:43

    Article  CAS  Google Scholar 

  45. Wullems, G.J., Molendijk, L. and Schilperoort, R.A. (1980) Theor. Appl. Genet. 56:203

    Article  Google Scholar 

  46. Márton, L., Wullems, G.J., Molendijk, L. and Schilperoort, R. A. (1979) Nature 277:129

    Article  Google Scholar 

  47. Wullems, G.J., Molendijk, L., Ooms, G., and Schilperoort, R. A. (1981) Cell 24:719

    Article  PubMed  CAS  Google Scholar 

  48. De Greve, H., Decraemer, H., Seurinck, J., Van Montagu, M., and Schell, J. (1981) Plasmid 6:235

    Article  PubMed  Google Scholar 

  49. De Greve, H., Leemans, J., Hernalsteens, J.P., Thia Thoong, L., De Beuckeleer, M., Willmitzer, L., Otten, L., Van Montagu, M., and Schell, J. (1982) submitted to Nature

    Google Scholar 

  50. Schröder, J., Schröder, G., Huisman, H., Schilperoort, R.A., and Schell, J. (1981) FEBS Lett. 129:166

    Article  Google Scholar 

  51. Otten, L., De Greve, H., Hernalsteens, J.P., Van Montagu, M., Schieder, O., Straub, J., and Schell, J. (1981) Mol. Gen. Genet. 183:209

    Article  PubMed  CAS  Google Scholar 

  52. Leemans, J., Shaw, C., Deblaere, R., De Greve, H., Hernal-steens, J.P., Maes, M., Van Montagu, M., and Schell, J. (1981) J. Mol. Appl. Genet. 1:149

    PubMed  CAS  Google Scholar 

  53. Maliga, P., Sz-Breznovits, A., Mârton, L., and Joò, F. (1975) Nature 255:401

    Article  PubMed  CAS  Google Scholar 

  54. Binding, H., Nehls, R., Schieder, O., Sopory, S.K., and Wen-zel, G. (1978) Physiol. Plant. 43:52

    Article  Google Scholar 

  55. Gresshoff, P.M., Skotnicki, M.L., and Rolfe, B.G. (1979) J. Bacteriol. 137:1020

    PubMed  CAS  Google Scholar 

  56. Wullems, G.J., Molendijk, L., Ooms, G., and Schilperoort, R. A. (1981) Proc. Natl. Acad. Sci. USA 78:4344

    Article  PubMed  CAS  Google Scholar 

  57. Kao, K.N. and Michayluk, M.R. (1975) Planta 126:105

    Article  CAS  Google Scholar 

  58. Nagy, J.I. and Maliga, P. (1976) Z. Pflanzenphysiol. 78:453

    Google Scholar 

  59. Zambryski, P., Depicker, A., Kruger, K., and Goodman, H. (1982) J. Mol. Appl. Genet. 1:361

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Max-Planck-Institut für Züchtungsforschung, Köln, Deutschland

    A. Wöstemeyer, L. Otten & J. Schell

  2. Laboratory for Genetics, State-University-Gent, Belgium

    H. De Greve & J. Schell

  3. Laboratory for Genetical Virology, Free University of Brussels, Belgium

    J. P. Hernalsteens, J. Leemans & M. Van Montagu

Authors
  1. A. Wöstemeyer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Otten
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. De Greve
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. P. Hernalsteens
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Leemans
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. Van Montagu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. J. Schell
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Washington State University, Pullman, Washington, USA

    Paul F. Lurquin  & Andris Kleinhofs  & 

Rights and permissions

Reprints and permissions

Copyright information

© 1983 Plenum Press, New York

About this chapter

Cite this chapter

Wöstemeyer, A. et al. (1983). Regeneration of Plants from Crown Gall Cells A step in the T-DNA mediated genetic engineering of plants. In: Lurquin, P.F., Kleinhofs, A. (eds) Genetic Engineering in Eukaryotes. NATO Advanced Science Institutes Series, vol 61. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4493-3_16

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-4493-3_16

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-4495-7

  • Online ISBN: 978-1-4684-4493-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation