Abstract
In these lectures, our primary focus will be on the phenomena of biological contractility and on how photon correlation spectroscopy can be used to study the basic molecular processes from which these phenomena arise. Photon correlation spectroscopy (PCS) permits the measurement of macromolecular diffusional relaxation times ranging from 10−6 to 102 seconds and these measurements can be made with great accuracy on small quantities of material in solutions, in gels, and in intact cells. Much has been learned about the structural dynamics of contractile proteins and organelles from photon correlation and transient electric birefringence (TEB) studies. The internal structural dynamics of contractile cells have also been successfully studied with PCS. However, the condensed state of the contractile proteins within the cell and the complexity of their interactions makes the interpretation of PCS data singularly difficult. PCS studies by themselves are not sufficient to lead to a solution of the problem of muscular contraction. On the other hand, when combined with time resolved X-ray diffraction studies and nuclear magnetic resonance studies, PCS studies can be highly useful in narrowing the field of possible models for the contractile mechanism.
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References
F. D. Carlson and D. R. Wilkie, “Muscle Physiology,” Prentice Hall, Inc., Englewood Cliffs, NJ (1974).
A. Weber and J. M. Murray, Physiol. Rev. 53: 612 (1973).
J. M. Squire, Ann. Rev. Bioeng. 4: 137 (1975).
H. G. Mannherz and R. S. Goode, Ann. Rev. Biochem. 45: 427 (1976).
R. A. Murphey, Ann. Rev. Physiol. 41: 737 (1979).
S. B. Marston, R. T. Tregear, C. D. Rodger and M. L. Clarke, J. Mol. Biol. 128: 11 (1979).
E. Taylor, CRC Crit. Rev. Biochem. 6: 103 (1979).
A. F. Huxley and R. M. Simmons, Nature (Lond.) 233: 533 (1971).
H. E. Huxley, Science 164: 1356 (1969).
A. F. Huxley and R. M. Simmons, C.S.H.S.2.B. 37: 669 (1972).
W. F. Harrington, Proc. Natl. Acad. Sci. U.S.A. 68: 685 (1971).
F. Oosawa et al., C.S.H.S.2.B. 37: 277 (1972).
Organization of the Cytoplasm“, C.S.H.S.2.B. XLVI (1982).
R. H. Mendelson, in: “Cell and Muscle Motility,” R. Dowben and J. Shea, eds., Plenum Publ. Corp. (1982) pg 257.
S. C. Harvey and H. C. Cheung, in: “Cell and Muscle Motility,” R. Dowben and J. Shea, eds., Plenum Publ. Corp. (1982) pg. 279.
D. D. Thomas et al., Proc. Natl. Acad. Sci. U.S.A. 72: 1729 (1975).
D. D. Thomas and R. Cooke, Biophys. J. 25: 19a (1979).
D. D. Thomas et al., Biophys J. 32: 873 (1980).
J. Borejdo et al., Proc. Natl. Acad. Sci. U.S.A. 76: 6346 (1979).
J. Borejdo and S. Putnam, Biochem. Biophys. Acta 459: 578 (1977).
H. E. Huxley and W. Brown, J. Mol. Biol. 30: 383 (1967).
J. C. Hazelgrove and H. E. Huxley, J. Mol. Biol. 77: 549 (1973).
J. C. Hazelgrove et al., Nature (Lond.) 261: 606 (1976).
R. W. Lymn and G. H. Cohen, Nature (Lond.) 258: 770 (1975).
H. E. Huxley et al., Proc. Natl. Acad. Sci. U.S.A. 78: 2297 (1981).
A. Elliott and G. Offer, J. Mol. Biol. 123: 505 (1978).
K. Takahashi, J. Biochem. 83: 905 (1978).
M. Burke et al., Biochem. 12: 701 (1973).
W. F. Harrington, Proc. Natl. Acad. Sci. U.S.A. 76: 5066 (1979).
J. Newman and H. Swinney, Biopolymers 15: 301 (1976).
F. D. CARLSON
H. Z. Cummins and P. N. Pusey, in: “Photon Correlation Spectroscopy and Velocimetry,” H. Z. Cummins and E.R. Pike, eds., Plenum Publ. Corp. (1977) pg. 164.
Y. Allen and A. Hochberg, Rev. Sci. Inst. 46: 381 (1975).
C. Montague and F. D. Carlson, in: “Advances in Enzymology,” S. Colowick and N. Kaplan, eds. (in press).
T. J. Racey, R. Hallett, and B. Nickel, Biophys. J. 35: 557 (1981).
C. J. Oliver, Adv. Phys. 27: 387 (1978).
P. Nieuwenhuysen, Macromolecules 11: 832 (1978).
J. G. de la Torre and V. A. Bloomfield, Biochem. 19: 5118 (1980).
S. J. Broersma, J. Chem. Phys. 32: 1626 (1960).
S. J. Broersma, J. Chem. Phys. 32: 1632 (1960).
J. Newman et al., J. Mol. Biol. 116: 593 (1977).
H. Yamakawa, “Modern Theory of Polymer Solutions,” Harper and Row, NY (1971).
P. J. de Gennes, “Scaling Concepts in Polymer Physics,” Cornell Univ. Press, Ithaca, NY (1979).
W. A. Wegener et al., J. Chem. Phys. 73: 4086 (1980).
W. A. Wegener, Biopolymers 19: 1899 (1980).
K. Zero and R. Pecora, Macromolecules, (in press).
C. C. Yang and R. Pecora, J. Chem. Phys. 72: 5333 (1980).
S. Highsmith et al., Biochem. 21: 1192 (1982).
M. Fujiwara et al., Reports on Progress in Polymer Physics in Japan 23: 531 (1980).
A. B. Fraser et al., Biochem. 14: 2207 (1975)
J. T. Yang and C. C. Yoo, Biochem. 16: 578 (1977).
C. Montague, K. W. Rhee, and F. D. Carlson, J. Cell Motility and Muscle Res. (in press).
U. Aebi et al., Nature 288: 296 (1980).
D. Suck, W. Kabsch, and H. G. Mannherz, Proc. Natl. Acad. Sci. U.S.A. 78: 4319 (1981).
F. D. Carlson and A. B. Fraser, J. Mol. Biol. 89: 273 (1974)
F. D. Carlson, J. Mol. Biol. 95: 139 (1975).
S. Fujimi, J. Phys. Soc. Japan 29: 751 (1970).
S. Ishiwata and S. Fujimi, J. Phys. Soc. Japan 31: 1601 (1971).
S. Fujimi and S. Ishiwata, J. Mol. Biol. 62: 251 (1971).
S. Ishiwata and S. Fujimi, J. Mol. Biol. 68: 511 (1972).
K. Mihashi, Arch. Biochem. Biophys. 107: 441 (1964).
F. Lanni, D. L. Taylor, and B. R. Ware, Biophys. J. 35: 351 (1981).
J. Newman and F.D. Carlson, Biophys. J. 29: 37 (1981).
B. M. Millman and P. M. Bennett, J. Mol. Biol. 103: 439 (1976).
T. Maeda and S. Fujimi, J. Phys. Soc. Japan 42: 1983 (1977).
T. Maeda and S. Fujimi, Macromolecules 14: 809 (1981).
A. Wegner, J. Mol. Biol. 131: 839 (1979).
C. Montague, unpublished data.
STUDY OF MUSCULAR CONTRACTION 457
R. W. Rosser et al., Macromolecules 11: 1239 (1978).
T. J. Herbert and F. D. Carlson, Biopolymers 10: 2231 (1971).
A. D’Albis and W. Gratzer, J. Biochem. 251: 2825 (1976).
S. Lowey et al., J. Mol. Biol. 42: 1 (1969).
J. G. de la Torre and V. A. Bloomfield, Biochem. 19: 5118 (1980).
S. Kobayasi and T. Totsuka, Error! Hyperlink reference not valid . Biophys. Acta 376: 375 (1975).
S. Highsmith et al., Proc. Natl. Acad. Sci. U.S.A. 74: 4986 (1977).
R. Mendelson et al., Biochem. 12: 2250 (1973).
J. Borejdo and M. F. Morales, Biophys. J. 20: 315 (1977).
F. D. Carlson, Biophys. J. 15: 633 (1975).
C. G. Dos Remedios et al., J. Gen. Physiol. 59: 103 (1972).
C. G. Dos Remedios et al., Proc. Natl. Acad. Sci. U.S.A. 69: 2542 (1972).
K. Guth, Biophys. Struct. Mech. 6: 81 (1980).
R. F. Bonner and F. D. Carlson, J. Gen. Physiol. 64:555 (1975). - -
R. C. Haskell and F. D. Carlson, Biophys. J. 33: 39 (1981).
S. Fujimi, in: Proc. NATO Adv. Study Inst. on Scattering Techniques Applied to Supramolecular and Nonequilibrium Systems, S. H. Chen, B. Chu, and R. Nossal, eds., Plenum Press (1981) pg 725.
B. Saleh, “Photoelectron Statistics,” Springer-Verlag, Inc. NY (1978).
A. M. Gordon et al., J. Physiol. (Lond) 184: 170 (1966).
F. D. Carlson, J. Mol. Biol. 95: 139 (1975).
R. C. Haskell and F. D. Carlson, unpublished.
T. Yanagida, J. Mol. Biol. 146: 539 (1981).
K. Mihashi and P. Wahl, FEBS Lett. 52: 8 (1975).
A. Eberstein and A. Rosenfalck, Acta Physiol. Scand. 57: 144 (1963).
D. L. Taylor, J. Cell Biol. 68: 497 (1976).
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© 1983 Plenum Press, New York
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Carlson, F.D. (1983). The Application of Quasi-Elastic Light Scattering to the Study of Muscular Contraction. In: Earnshaw, J.C., Steer, M.W. (eds) The Application of Laser Light Scattering to the Study of Biological Motion. NATO Advanced Science Institutes Series, vol 59. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4487-2_28
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DOI: https://doi.org/10.1007/978-1-4684-4487-2_28
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