Abstract
Studies of interactions between neurotransmitters and their receptors would be greatly facilitated by a method to obtain cell fractions enriched with cells that contain a high density of receptors specific for the neurotransmitter. Here we report the use of a fluorescence activated cell sorter to prepare fractions of pituitary cells that contain a high density of high affinity, specific receptors for the decapeptide, luteinizing hormone releasing hormone (LHRH). Since no enzymatic procedures are employed, the dispersed cells permit both binding and biological responses to be analysed immediately without requiring the usual time of 16–48hr for recovery.
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References
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Speight, A., Fink, G. 1981. Changes in responsiveness of dispersed pituitary cells to luteinizing hormone releasing hormone at different times of the oestrous cycle of the rat. J. Endocr. 89: 129–134.
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© 1983 Plenum Press, New York
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MacInnes, D.G., Green, D.K., Harmar, A., Nairn, E.G., Fink, G. (1983). Studies of Neurotransmitter Receptor Interactions Using Quantitative Video Intensification Microscopy (V.I.M.). In: Earnshaw, J.C., Steer, M.W. (eds) The Application of Laser Light Scattering to the Study of Biological Motion. NATO Advanced Science Institutes Series, vol 59. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4487-2_10
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DOI: https://doi.org/10.1007/978-1-4684-4487-2_10
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4489-6
Online ISBN: 978-1-4684-4487-2
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