Abstract
To develop an assay for bromocriptine (2-bromo- α-ergokryptine), different hapten compounds were used to produce various animal antisera. Since all these were predominantly specific to the tricyclic peptide moiety of bromocriptine, the affinity constant Kwas considered a sufficient criterion for selecting a high energy antibody. The K value for the favoured antiserum (#270979) was 0.9 x 10 9 1/M. With the favoured tracer ligand, a detection limit of 0.17 ng/ml was achieved for bromocriptine in equilibrium with its epimer bromocriptinine. Metabolites such as the 8′-hydroxy derivative show virtually no cross-reaction.
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References
Weber, H.P. (1980) in Ergot Compounds and Brain Function [VoL 23, Adv. Biochem. Psychopharmacol] (Goldstein, M., Calne, D.B., Lieberman, A. & Thorner, M.O., eds.) , Raven Press, New York, pp. 25–34.
Flückiger, E. & Wagner, H.-R. (1968)Experientia24, 1130.
Hökfelt, T. & Fuxe, K. (1972) in Brain-Endocrine Interaction (Knigge, K.M., Scott, D.E. & Weindl, A., eds.) , Karger, Basel, pp. 181–223.
Calne, D.B., Teychenne, P.F., Claveria, LE., Eastman, R., Greenacre, J.K. & Petrie, A. (1974)Br. Med. J. 4, 442–444
Yalow, R.S. & Berson, S.A. (1971) In Competitive Protein Binding Assays (Odell, W.D. & Daughaday, W.H., eds.) , Lippincott, Philadelphia, pp. 1–21.
Levy, A., Kawashima, K. & Spector, S. (1976)Life Sci. 19, 1421–1429.
Rosenthaler, J. & Munzer, H. (1976)Experientia32, 234–235.
Rosenthaler, J., Nimmerfall, F., Sigrist, R. & Munzer, H. (1977)Eur. J. Biochem. 80, 603–609.
Rosenthaler, J., Pacha, W.L., Koeberle, S. & Beveridge, T. (1980) in Phenothiazines and Structurally Related Drugs: Basic and Clinical Studies (Usdin, E., Eckert, H. & Forrest, I.S., ) Elsevier/North Holland, New York, pp. 121–124
Eckert, H., Kiechel, J.R., Rosenthaler, J., Schmidt, R. & Schreier, E. (1978) in Ergot Alkaloids and Related Compounds, Vol. 49, Handbook of Experimental Pharmacology (Berde, B. & Schild, H.O., eds.) Springer-Verlag, Berlin, pp. 780–782.
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© 1983 Plenum Press, New York
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Rosenthaler, J., Munzer, H., Voges, R. (1983). Immunoassay of Bromocriptine and Specificity of Antibody: Criteria for Choice of Antiserum and Marker Compound. In: Reid, E., Leppard, J.P. (eds) Drug Metabolite Isolation and Determination. Methodological Surveys in Biochemistry and Analysis, vol 12. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4484-1_25
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DOI: https://doi.org/10.1007/978-1-4684-4484-1_25
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