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Isolation of Conjugates and Identification of the Attached Groups

  • Chapter
Drug Metabolite Isolation and Determination

Part of the book series: Methodological Surveys in Biochemistry and Analysis ((ANAY,volume 12))

Abstract

Metabolic Phase II reactions convert a wide variety of drugs, endogenous substances and environmental chemicals into conjugates. The application of various isolation and characterization procedures to the analysis of several types of conjugates is considered, including:

  1. (a)

    conjugates with glucuronic acid, special attention being paid to some novel types, to possible formation of isomeric structures in ester-type glucuronides, and to the use of β-glucuronidase in the assay of glucuronides;

  2. (b)

    conjugates with amino acids, and particularly glutathione conjugates and mercapturic acids, mention being made of substrate specificity of glutathione S-transferase;

  3. (c)

    sulphoconjugates as products of conjugation of steroids and phenols.

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Author information

Authors and Affiliations

  1. “Ruder Bošković” Institute, 41000, Zagreb, Yugoslavia

    Jelka Tomašić

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  1. Jelka Tomašić
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Editors and Affiliations

  1. University of Surrey, UK

    Eric Reid  & J. P. Leppard  & 

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© 1983 Plenum Press, New York

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Tomašić, J. (1983). Isolation of Conjugates and Identification of the Attached Groups. In: Reid, E., Leppard, J.P. (eds) Drug Metabolite Isolation and Determination. Methodological Surveys in Biochemistry and Analysis, vol 12. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4484-1_19

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  • DOI: https://doi.org/10.1007/978-1-4684-4484-1_19

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-4486-5

  • Online ISBN: 978-1-4684-4484-1

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