Abstract
The background to the procedure shown in Plate 1 is that alumina is an essential tool in the measurement of catecholamines and analogues since they are fairly specifically adsorbed at slightly alkaline pH and are readily eluted at acidic pH. After the requisite acid pre-wash, alumina is a powder with a large range of particle sizes. It is essential to remove the finer particles, since they drastically reduce the flow rate through an alumina column. The traditional way [1] entailing washing with water is inefficient as it removes little more than colloidal material, and is very-time consuming.
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Reference
Weil-Malherbe, H. (1961) in Methods in Medical Research, Vol. 9 (Quastel, J.H., ed.) , Year Book Medical Publishers, Chicago, pp. 130–146.
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© 1983 Plenum Press, New York
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Goodwin, B.L. (1983). A Note on Rapid Removal of Fine Particles from Alumina for Column Chromatography. In: Reid, E., Leppard, J.P. (eds) Drug Metabolite Isolation and Determination. Methodological Surveys in Biochemistry and Analysis, vol 12. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4484-1_10
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DOI: https://doi.org/10.1007/978-1-4684-4484-1_10
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