Inflammatory Response of LPS-Hyporesponsive and LPS-Responsive Mice to Challenge with Gram-Negative Bacteria Salmonella Typhimurium and Klebsiella Pneumoniae

  • T. J. MacVittie
  • A. D. O’Brien
  • R. I. Walker
  • S. R. Weinberg
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 155)


The murine response to lipopolysaccharide (LPS) or endotoxin is determined by the allelic form of the Lps gene carried by the host (1,2). Mice that are homozygous for the defective Lps d allele, such as C3H/HeJ (HeJ) animals, respond to only high doses of endotoxin, whereas mice that are homozygous or heterozygous for the normal Lps n allele, e.g., C3H/HeN (HeN) mice, react to low-dose challenge. Thus, HeJ mice are insensitive to quantities of LPS that elicit mitogenic, inflammatory, hemopoietic, or lethal effects in HeN mice (3–10). Furthermore, the nature of the cellular influx into the peritoneum of LPS-inoculated HeN and HeJ mice differs. Low doses of LPS (1–10 pg) induce an early polymorphonuclear (PMN) increase, followed by a rapid rise in macrophages and macrophage colony-forming cells in the HeJ peritoneal cavity (7,11,12). By contrast, a relatively small PMN infiltrate is evident in the HeN peritoneal inflammatory response, and the onset of the macrophage influx is delayed compared to the HeJ response.


Peritoneal Cell Mouse Kill Cellular Influx Naval Medical Research Institute Macrophage Influx 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Watson, J., Riblet, R., and Taylor, B. A., J. Immunol. 118:2088, 1977.PubMedGoogle Scholar
  2. 2.
    Watson, J., Kelly, K., Largen, M., and Taylor, B. A., J. Immunol. 120:422, 1978.PubMedGoogle Scholar
  3. 3.
    Sultzer, B. M., J. Immunol. 103:32, 1969.PubMedGoogle Scholar
  4. 4.
    Watson, J., and Riblet, R., J. Exp. Med. 140:1147, 1974.PubMedCrossRefGoogle Scholar
  5. 5.
    Glode, M. L., Jacques, A., Mergerhagen, S. E., and Rosenstreich, D. L., J. Immunol. 119:162, 1977.PubMedGoogle Scholar
  6. 6.
    Doe, W. F., and Hensen, P. M., J. Immunol. 123:2304, 1979.PubMedGoogle Scholar
  7. 7.
    Moeller, G. R., Terry, L., and Snyderman, R., J. Immunol. 120:116, 1978.PubMedGoogle Scholar
  8. 8.
    Apte, R. M., and Pluznik, D. H., J. Cell. Physiol. 89:313, 1976.PubMedCrossRefGoogle Scholar
  9. 9.
    Boggs, S. S., Boggs, D. R., and Joyce, R. A., Blood 55:444, 1980.PubMedGoogle Scholar
  10. 10.
    MacVittie, T. J., and Weinberg, S. R., in “Experimental Hematology Today” (S. J. Baum and G. D. Ledney, eds.), pp. 19–28, Springer-Verlag, New York, 1980.Google Scholar
  11. 11.
    Sultzer, B.M., and Goodman, G. W., in “Microbiology” (D. Schlesinger, ed.), p. 304, American Society of Microbiology, Washington, D. C., 1977.Google Scholar
  12. 12.
    MacVittie, T. J., and Weinberg, S. R., in “Genetic Control of Natural Resistance to Infection and Malignancy” (E. Skamene, P. A. L. Kongshavn and M. Landy, eds.), pp. 511–518, Academic Press, New York, 1980.Google Scholar
  13. 13.
    Robson, H. G., and Vas, S. I., J. Infect. Dis. 126:378, 1972.PubMedCrossRefGoogle Scholar
  14. 14.
    Von Jeney, N. E., Gunter, E., and Jann, K., Infect. Immun. 15:26, 1977.Google Scholar
  15. 15.
    O’Brien, A. D., Rosenstreich, D. L., Scher, I., Campbell, G. H., MacDermott, R. P., and Formal, S. B., J. Immunol. 124:20, 1980.PubMedGoogle Scholar
  16. 16.
    Chedid, L., Parent, M., Damals, C., Juy, D., and Galelli, A., Infect. Immun. 13:722, 1976.PubMedGoogle Scholar
  17. 17.
    Gianella, R. A., Broitman, S. A., and Zamcheck, N., Am. J. Dig. Dis. 16:1007, 1971.CrossRefGoogle Scholar
  18. 18.
    Reed, L. J., and Muench, H., Am. J. Hyg. 27:493, 1938.Google Scholar
  19. 19.
    Jerrells, T. R., and Osterman, J. V., Infect. Immun. 31:1014, 1981.PubMedGoogle Scholar
  20. 20.
    O’Brien, A. D., Scher, I., and Formal, S. B., Infect. Immun. 25:513, 1979.PubMedGoogle Scholar
  21. 21.
    O’Brien, A. D., Metcalf, E. S., and Rosenstreich, D. L., Cell. Immunol., 67:325, 1982.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • T. J. MacVittie
    • 1
  • A. D. O’Brien
    • 2
  • R. I. Walker
    • 3
  • S. R. Weinberg
    • 1
  1. 1.Experimental Hematology DepartmentArmed Forces Radiobiology Research InstituteBethesdaUSA
  2. 2.Department of MicrobiologyUniformed Services University of Health SciencesBethesdaUSA
  3. 3.Medical Microbiology BranchNaval Medical Research InstituteBethesdaUSA

Personalised recommendations