Regulation of Bone-Marrow Macrophage Proliferation

  • David A. Hume
  • Siamon Gordon
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 155)


Macrophages are derived from pluripotent bone marrow stem cells (1). Their proliferation and differentiation can be induced in vitro by the addition of “conditioned media” that contain colony-stimulating factors (2–6). Colony-stimulating factors (CSF) are normally assayed by their ability to promote colony formation from stem cells in semi-solid (soft agar or methylcellulose) medium. Two types of colony-stimulating factors that influence macrophage growth have been identified. CSF-1 (isolated from mouse L-cell conditioned medium or human urine) gives rise to colonies entirely composed of macrophages while CSF-II (e.g. from WEHI-3 conditioned medium or endotoxinlung conditioned medium) yields mixed colonies of granulocytes and macrophages (2–6).


Conditioned Medium Thymidine Incorporation Roller Bottle Thymidine Incorporation Assay Mixed Coloni 
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  1. 1.
    Van Furth, R., Annals N.Y. Acad. Sci. 278, 161.Google Scholar
  2. 2.
    Metcalf, D., in: “In Vitro Cloning of Normal and Leukemic Cells” Springer-Verlag, Berlin, 1971.Google Scholar
  3. 3.
    Nicola, N. A., Burgess, A. W., and Metcalf, D., J. Biol. Chem. 254:5290, 1979.PubMedGoogle Scholar
  4. 4.
    Byrne, P. V., Guilbert, L. J., and Stanley, E. R., J. Cell Biol. 91:848, 1981.PubMedCrossRefGoogle Scholar
  5. 5.
    Stanley, E. R., and Guilbert, L. J., J. Immunol. Methods 42:253, 1981.PubMedCrossRefGoogle Scholar
  6. 6.
    Tushinski, R. J., Oliver, I. T., Guilbert, L. J., Tynan, P. W., Warner, J. R., and Stanley, E. R., Cell 28:71, 1982.PubMedCrossRefGoogle Scholar
  7. 7.
    Lin, H-S, and Gordon, S., J. Exp. Med. 150:231, 1979.PubMedCrossRefGoogle Scholar
  8. 8.
    Lin, H-S, Gordon, S., Chen, D. M., and Kurtz, M., J. Exp. Med. 153:488, 1981.PubMedCrossRefGoogle Scholar
  9. 9.
    Folkman, J., and Moscona, A., Nature (London) 273:345, 1978.CrossRefGoogle Scholar
  10. 10.
    Holley, R. W., Nature (London) 258:487, 1975.CrossRefGoogle Scholar
  11. 11.
    Boynton, A. L., and Whitfield, J. F., Cancer Res. 38:1237, 1978.PubMedGoogle Scholar
  12. 12.
    Swierenga, S. H. H., Whitfield, J. F., and Karasaki, S., Proc. Natl. Acad. Sci. U.S.A. 75:6069, 1978.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • David A. Hume
    • 1
  • Siamon Gordon
    • 1
  1. 1.Sir William Dunn School of PathologyOxford OX1UK

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