Characteristics of Human Monocyte Subpopulations with the use of Flow Cytometer
Monocytes isolated by counterflow centrifugation have a bimodal volume distribution with the large monocytes predominantly Fc receptor (FcR) positive and active in ADCC assays (1). Volume analysis of human monocytes with a multiparameter cell sorter has revealed three distinct populations with different chemotactic capabilities (2).
KeywordsHuman Monocyte Scatchard Plot Fluorescein Diacetate Scattered Light Intensity Average Fluorescence Intensity
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- 4.Salzman, G. C., Mullaney, P. F., and Price, B. J., in “Flow Cytometry and Sorting” (M. R. Melamed, P. F. Mullaney and M.L. Mendelsohn, eds.), pp. 105–124, Wiley Medical Publication, New York, 1979.Google Scholar