Changes in Surface Markers of Human Monocytes Following Their In Vitro Maturation to Macrophages
Peripheral blood monocytes are a transitory population between bone marrow promonocytes and tissue macrophages (1,2). Blood monocytes have several characteristics which assign them to the macrophage lineage. However, each of the macrophage markers is not always expressed on the entire monocyte population (3,4) and some markers and functions are altered during monocyte maturation or activation (5,6,7). In vitro cultivation of human monocytes is accompanied by a series of changes which results in their transition to macrophages (8). In the present study, we analyzed membrane markers on freshly obtained monocytes and on mature macrophages. These were the receptor for peanut agglutinin (PNA), which is associated with cell maturation in T lymphocytes (9) and the HLA-DR which is associated with some immune functions (10–12).
KeywordsPeanut Agglutinin Mature Macrophage Monocyte Macrophage Fluorescent Positive Cell Transitory Population
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