Separation and Characterization of Guinea Pig Monocyte Subsets using Counter-Flow Centrifugation Elutriation (CCE)

Noga, S. J.; Normann, S. J.; Weiner, R.

doi:10.1007/978-1-4684-4394-3_21

Separation and Characterization of Guinea Pig Monocyte Subsets using Counter-Flow Centrifugation Elutriation (CCE)

S. J. Noga³,
S. J. Normann³ &
R. Weiner³

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 155))

Abstract

Counter-flow centrifugation elutriation (CCE) has been used to separate human monocytes into two populations that differ in size, Fc receptors (FcR), antibody-dependent cellular cytotoxicity (ADCC), and native or acquired tumoricidal activity (1–4). To determine whether a similar monocyte heterogeneity exists in species other than man, we examined guinea pig (GP) monocytes. Unlike mouse and rat, GP peripheral blood has a white cell composition similar to humans (5). Our studies suggest that three distinct GP monocyte fractions can be isolated by CCE that differ in adherence, phagocytosis, and non-specific esterase activity as well as modal volume (MV), FcR’s, and acid phosphatase activity.

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Authors and Affiliations

Department of Pathology and Division of Medical Oncology, University of Florida, Gainesville, Florida, 32610, USA
S. J. Noga, S. J. Normann & R. Weiner

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S. J. Noga
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S. J. Normann
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R. Weiner
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Editors and Affiliations

College of Medicine, University of Florida, Gainesville, Florida, USA
Sigurd J. Normann
Department of Medicine, Swiss Research Institute, Davos, Switzerland
Ernst Sorkin

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Noga, S.J., Normann, S.J., Weiner, R. (1982). Separation and Characterization of Guinea Pig Monocyte Subsets using Counter-Flow Centrifugation Elutriation (CCE). In: Normann, S.J., Sorkin, E. (eds) Macrophages and Natural Killer Cells. Advances in Experimental Medicine and Biology, vol 155. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4394-3_21

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DOI: https://doi.org/10.1007/978-1-4684-4394-3_21
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4396-7
Online ISBN: 978-1-4684-4394-3
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