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Effect of LPS on Carbohydrate and Lipid Metabolism

  • Chapter
Beneficial Effects of Endotoxins

Abstract

Administration of endotoxin, a lipopolysaccharide (LPS) extracted from cell walls of gram-negative bacteria, elicits alterations in various metabolic parameters in many mammalian species, including man. Studies of the mechanism of metabolic alterations induced by LPS are important because release of endotoxin may contribute to the effects of sepsis, and clinical or experimental infections may alter the course of other types of trauma, shock, or injury. The overall metabolic and endocrine effects of endotoxicosis are predominantly catabolic. In general, the endocrine changes appear to be directed toward water and salt conservation, maintenance of blood pressure, and the mobilization of fuel primarily from carbohydrate stores. Eventually the breakdown of muscle protein follows, providing additional substrate for gluconeogenesis and satisfying the energy requirements of peripheral tissues and the brain.

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References

  • Adelye, A. G., Al-Fibouri, L. M., Furman, B. L., and Parratt, J. R., 1981, Endotoxin-induced metabolic changes in the conscious, unrestrained rat: Hypoglycemia and elevated blood lactate concentrations without hyperinsulinemia, Circ. Shock 8:543–550.

    Google Scholar 

  • Bagby, G. J., and Spitzer, J. A., 1980, Lipoprotein lipase activity in rat heart and adipose tissue during endotoxic shock, Am. J. Physiol. 238:H325–H330.

    PubMed  CAS  Google Scholar 

  • Berry, L. J., 1971, Metabolic effects of bacterial endotoxin, in: Microbial Toxins, Vol. V (S. Kadis, G. Weinbaum, and S.J. Ajl, eds.), pp. 165–208, Academic Press, New York.

    Google Scholar 

  • Berry, L. J., Smythe, D. S., and Colwell, L. S., 1968, Inhibition of hepatic enzyme induction as a sensitive assay for endotoxin,J. Bacteriol. 96:91–99.

    Google Scholar 

  • Bitensky, M. W., Gorman, R. E., and Thomas, L., 1971, Selective stimulation of epinephrine-response adenyl-cyclase in mice by endotoxin, Proc. Soc. Exp. Biol. Med. 138:773.

    PubMed  CAS  Google Scholar 

  • Blackard, W. G., Anderson, J. H., and Spitzer, J. J., 1976, Hyperinsulinism in endotoxin shock dogs, Metabolism 25:675–684.

    Article  PubMed  CAS  Google Scholar 

  • Blackmore, P. F., Assimacopoulos-Jeannet, F., Chan, T. M., and Exton, J. H., 1979, Studies on a-adrenergic activation of hepatic glucose oxidation,J. Biol. Chem. 254:2828–2834.

    PubMed  CAS  Google Scholar 

  • Buchanan, B. J., and Filkins, J. P., 1976, Insulin secretion and the carbohydrate metabolic alterations of endotoxemia, Circ. Shock 3:267–280.

    CAS  Google Scholar 

  • Cameron, G. R., Relefield, M. E., and Wilson, J., 1940, Pathological changes produced in rats and mice by a toxic fraction derived from Bacterium typhimurium, J. Pathol. Bacteriol. 51:223–233.

    Article  CAS  Google Scholar 

  • Chen, J. J., Babcock, D. F., and Lardy, H. A., 1978, Norepinephrine, vasopressin, glucagon and A23187 induce efflux of calcium ion from an exchangeable pool in isolated rat hepatocytes, Proc. Natl. Acad. Sci. USA 75:2234–2238.

    Article  PubMed  CAS  Google Scholar 

  • Cline, M. J., Melmon, K. L., Davis, W. C., and Williams H. E., 1968, Mechanism of endotoxin interaction with human leukocytes, Br. J. Haematol. 15:539–547.

    Article  PubMed  CAS  Google Scholar 

  • Cohn, Z. A., and Morse, S. I., 1960, Functional and metabolic properties of polymorphonuclear leucocytes. II. The influence of lipopolysaccharide endotoxin, J. Exp. Med. 3:689–704.

    Article  Google Scholar 

  • Connor, J., Fine, J., Kusano, K., McCrea, M. J., Parnas, I., and Prosser, C. L., 1973, Potentiation by endotoxin of responses associated with increases in calcium conductance, Proc. Natl. Acad. Sci. USA 70:3301–3304.

    Article  PubMed  CAS  Google Scholar 

  • Ferguson, J. L., Spitzer, J. J., and Miller, H. I., 1978, Effects of endotoxin on regional blood flow in the unanesthetized guinea pig, J. Surg. Res. 23:236–243.

    Article  Google Scholar 

  • Filkins, J. P., and Buchanan, B. J., 1977, In vivo vs in vitro effects of endotoxin on glycogenosis, gluconeogenesis and glucose utilization, Proc. Soc. Exp. Biol. Med. 155:216–218.

    PubMed  CAS  Google Scholar 

  • Filkins, J. P., and Cornell, R. P., 1974, Depression of hepatic gluconeogenesis and the hypoglycemia of endotoxic shock, Am. J. Physiol. 227:778–782.

    PubMed  CAS  Google Scholar 

  • Filkins, J. P., and Figlewicz, D. P., 1979, Increased insulin responsiveness in endotoxico-sis, Circ. Shock 6:1–6.

    PubMed  CAS  Google Scholar 

  • Fredholm, B. B., 1971, The effect of lactate in canine subcutaneous adipose tissue in situ, Acta Physiol. Scand. 81:110–123.

    Article  CAS  Google Scholar 

  • Gartner, S. L., 1975, Hepatic levels of cyclic AMP in normal and lead-sensitized rats after treatment with bacterial endotoxin, Experientia 31:566.

    Article  PubMed  CAS  Google Scholar 

  • Gimpel, L., Hodgins, D. S., and Jacobson, E. D., 1974, Effects of endotoxin on hepatic adenylate cyclase activity, Circ. Shock 1:21.

    Google Scholar 

  • Hamosh, M., and Shapiro, B., 1960, The mechanism of glycogenolytic action of endotoxin, Br. J. Exp. Pathol. 41:372.

    PubMed  CAS  Google Scholar 

  • Hikawyj-Yevich, I., and Spitzer, J. A., 1977, The role of adrenergic receptors and Ca2+ in the action of endotoxin on human fat cells,J. Surg. Res. 23:233–238.

    Article  PubMed  CAS  Google Scholar 

  • Hinshaw, L. B., Archer, L. T., Beller, B. K., White, G. L., Schroeder, T. M., and Holmes, D. D., 1977, Glucose utilization and role of blood in endotoxin shock, Am. J. Physiol. 233:E71–E79.

    PubMed  CAS  Google Scholar 

  • Holley, D. C., and Spitzer, J. A., 1980, Insulin action and binding in adipocytes exposed to endotoxin in vitro and in vivo, Circ. Shock 7:3–13.

    CAS  Google Scholar 

  • Issekutz, B., Jr., Miller, H. I., Paul, P., and Rodahl, K., 1965, Effect of lactic acid on free fatty acids and glucose oxidation in dogs, Am. J. Physiol. 209:1137–1144.

    PubMed  CAS  Google Scholar 

  • Kelleher, D. L., Gagby, G. J., and Spitzer, J. J., 1981, Metabolic and endocrine alterations following endotoxin administration in normal and diabetic rats, in: Advances in Physiological Sciences, Vol. 26 (Z. Biro, A. G. B. Kovach, J. J. Spitzer, and H. B. Stoner, eds.), pp. 181–189, Pergamon Press/Akademiai Kiado, Budapest.

    Google Scholar 

  • Kelleher, D. L., Bagby, G. J., Fong, B. C., and Spitzer, J. J., 1982, Glucose turnover 5 hours following endotoxin administration to normal and diabetic rats, Metabolism 31:252–257.

    Article  PubMed  CAS  Google Scholar 

  • Kinney, J. M., and Felig, P., 1979, The metabolic response to injury and infection, in: Endocrinology (L.J. DeGroot, ed.), pp. 1963–1985, Grune & Stratton, New York.

    Google Scholar 

  • Kovach, A. G. B., Kovach, E., Sandor, P., Spitzer, J. A., and Spitzer, J. J., 1976, Metabolic responses to localized ischemia in adipose tissue, J. Surg. Res. 20:37–44.

    Article  PubMed  CAS  Google Scholar 

  • Kuttner, R., and Spitzer, J. J., 1978, Gluconeogenesis from alanine in endotoxin-treated dogs,J. Surg. Res. 25:166.

    Article  PubMed  CAS  Google Scholar 

  • Landy, M., Whitby, J. L., Michale, J. G., Woods, M. W., and Newton, W. L., 1962, Effect of bacterial endotoxin in germ free mice, Proc. Soc. Exp. Biol. Med. 109:352–356.

    PubMed  CAS  Google Scholar 

  • LaNoue, K., Mason, A. D., Jr., and Daniels, J., 1968, The impairment of gluconeogenesis by gram negative infection, Metabolism 17:606–611.

    Article  PubMed  CAS  Google Scholar 

  • Leach, G. J., and Spitzer, J. A., 1981, Endotoxin induced alterations in glucose transport in isolated adipocytes, Biochim. Biophys. Acta 648:71–79.

    Article  PubMed  CAS  Google Scholar 

  • Little, J. R., Goto, M., and Spitzer, J. J., 1970, Effect of ketones on metabolism on FFA by dog myocardium and skeletal muscle, Am. J. Physiol. 219:1458–1463.

    PubMed  CAS  Google Scholar 

  • Liu, M.-S., and Spitzer, J. J., 1977, Myocardial fatty acid and lactate kinetics after E. coli endotoxin administration, Circ. Shock 4:191–200.

    PubMed  CAS  Google Scholar 

  • Liu, M.-S., Long, W. M., and Spitzer, J. J., 1981, Influence of E. coli endotoxin on palmi-tate, glucose and lactate utilization by isolated dog heart myocytes, in: The Pathology of Endotoxin at the Cellular Level (J. A. Majda and R. J. Person, eds.), pp. 115–121, Liss, New York.

    Google Scholar 

  • McDonald, J. M., Burns, D. E., and Jarett, L., 1978, Ability of insulin to increase calcium uptake by adipocyte endoplasmic reticulum,J. Biol. Chem. 253:3504–3508.

    PubMed  CAS  Google Scholar 

  • Martin, S. P., McKinney, G. R., and Green, R., 1955, The metabolism of human polymorphonuclear leukocytes, Ann. N.Y. Acad. Sci. 59:996–1002.

    Article  PubMed  CAS  Google Scholar 

  • Menten, M. L., and Manning, H. M., 1924, Blood sugar studies on rabbits injected with organisms of the enteritidis-parathyroid B group,J. Med. Res. 44:676–681.

    Google Scholar 

  • Merrill, G. F., and Spitzer, J. J., 1978, Glucose and lactate kinetics in guinea pigs following Escherichia coli endotoxin administration, Circ. Shock 5:11–21.

    PubMed  CAS  Google Scholar 

  • Motsay, G. J., Alho, A. V., Dietzmann, R. H., and Lillehei, R. C., 1971, Pathophysiology and therapy of endotoxin (septic) shock, in: Emergency Medical Management (S. Spitzer, W. W. Oaks, and J. H. Moyer, eds.), pp. 247–267, Grune & Stratton, New York.

    Google Scholar 

  • Neufeld, H. A., Pace, J. A., and White, F. E., 1976, The effect of bacterial infections on ketone concentrations in rat liver and blood and on free fatty acid concentrations in rat blood, Metabolism 25:877–884.

    Article  PubMed  CAS  Google Scholar 

  • Nicolas, G. G., Mela, L. M., and Miller, L. D., 1972, Shock-induced alterations of mitochondrial membrane transport, Ann. Surg. 176:579–584.

    Article  Google Scholar 

  • Person, R. J., 1977, Endotoxin alters spontaneous transmitter release at frog neuromuscular junction, J. Neurosa. Res. 3:63–72.

    Article  CAS  Google Scholar 

  • Rayfield, E. J., Curnow, R. T., Reinhard, D., and Kochiceril, N. M., 1977, Effects of acute endotoxemia on glucoregulation in normal and diabetic subjects,J. Clin. Endocrinol. Metab. 45:513–521.

    Article  PubMed  CAS  Google Scholar 

  • Raymond, R. M., Harkema, J. M., and Emerson, T. E., Jr., 1981, Mechanism of increased glucose uptake by skeletal muscle during E. coli endotoxin shock in the dog, Circ. Shock 8:77–93.

    PubMed  CAS  Google Scholar 

  • Ribi, E., Haskins, W. T., Landry, M., and Milner, K. C., 1961, Symposium on bacterial endotoxin. I. Relationship of chemical composition to biological activity, Bacteriol. Rev. 25:427–436.

    PubMed  CAS  Google Scholar 

  • Romanosky, A. J., Bagby, G. J., Bockman, E. L., and Spitzer, J. J., 1980a, Increased skeletal muscle glucose uptake and lactate release following E. coli endotoxin administration, Am. J. Physiol. 239:E311–E316.

    PubMed  CAS  Google Scholar 

  • Romanosky, A. J., Bagby, G. J., Bockman, E. L., and Spitzer, J. J., 1980b, Free fatty acid utilization by skeletal muscle after endotoxin administration, Am. J. Physiol. 239:E391–E395.

    PubMed  CAS  Google Scholar 

  • Romanosky, A. J., McGuinness, O., and Spitzer, J. J., 1982, Ketone body (KB) clearance following endotoxin (ET) administration in dogs, Fed. Proc. 41:1133.

    Google Scholar 

  • Schimmel, R. J., 1975, The role of calcium ion in epinephrine activation of lipolysis, Horm. Metab. Res. 8:195–201.

    Article  Google Scholar 

  • Scholl, R. A., Lang, C. H., Spitzer, J. J., and Bagby, G. J., 1982, Tissue lipoprotein lipase (LPL) activities in rats challenged with endotoxin, live E. coli, or peritonitis, Fed. Proc. 41:1134.

    Google Scholar 

  • Scott, J. C., Weng, J. T., and Spitzer, J. J., 1973, Myocardial metabolism during endotoxic shock, in: Neurohumoral and Metabolic Aspects of Injury (A. G. B. Kovach, B. Stoner, and J. J. Spitzer, eds.), pp. 375–386, Plenum Press, New York.

    Google Scholar 

  • Shands, P. W., Jr., Miller, W., Martin, H., and Senterfilt, V., 1969, Hypoglycemic activity of endotoxin. II. Mechanism of the phenomenon in BCG-infected mice, J. Bacteriol. 98:495–501.

    Google Scholar 

  • Snyder, J. S., Deters, M., and Ingle, J., 1971, Effect of endotoxin on pyruvate kinase activity in mouse liver, Infect. Immun. 4:138–142.

    PubMed  CAS  Google Scholar 

  • Soulsby, M. E., Bruin, F. D., Looney, T. J., and Hess, M. L., 1978, Influence of endotoxin on myocardial calcium transport and the effect of augmented venous return, Circ. Shock 5:23–34.

    PubMed  CAS  Google Scholar 

  • Spitzer, J. A., 1974, Endotoxin-induced alterations in isolated fat cells: Effect on norepinephrine-stimulated lipolysis and cyclic 3′5′-adenosine monophosphate accumulation, Proc. Soc. Exp. Biol. Med. 145:186–191.

    PubMed  CAS  Google Scholar 

  • Spitzer, J. A., 1981, Altered insulin sensitivity in endotoxin and septic shock, in: Advances in Physiological Sciences, Vol. 26 (Z. Biro, A. G. B. Kovach, J. J. Spitzer, and H. B. Stoner, eds.), pp. 143–150, Pergamon Press/Akademiai Kiado, Budapest.

    Google Scholar 

  • Spitzer, J. A., and Holley, D. C., 1979, Alterations in insulin action by endotoxin in vitro, in: Advances in Shock Research, Vol. 2 (W. Schumer, J. J. Spitzer, and B. E. Marshall, eds.), pp. 129–136, Liss, New York.

    Google Scholar 

  • Spitzer, J. A., Archer, L., Greenfield, L. J., Hinshaw, L. B., and Spitzer, J. J., 1972, Metabolism of the nonhepatic splanchnic area in baboons and the effects of endotoxin, Proc. Soc. Exp. Biol. Med. 141:21–25.

    PubMed  CAS  Google Scholar 

  • Spitzer, J. A., Kovach, A. G. B., Sandor, P., Spitzer, J. J., and Storck, R., 1973a, Adipose tissue and endotoxin shock, Acta Physiol. Acad. Sci. Hung. 44:183–194.

    PubMed  CAS  Google Scholar 

  • Spitzer, J. A., Kovach, A. G. B., Roseli, S., Sandor, P., Spitzer, J. J., and Storck, R., 1973b, Influence of endotoxin on adipose tissue metabolism, in: Advances in Experimental Medicine and Biology, Vol. 33 (A. G. B. Kovach, H. B. Stoner, and J. J. Spitzer, eds.), pp. 337–344, Plenum Press, New York.

    Google Scholar 

  • Spitzer, J. J., 1979, Lipid metabolism in endotoxic shock, Circ. Shock 1(Suppl.):69–79.

    CAS  Google Scholar 

  • Spitzer, J. J., 1981, Lactate-fatty acid competition in the heart during shock, in: Advances in Physiological Sciences, Vol. 8 (A. G. B. Kovach, E. Monos, and G. Rubanyi, eds.), pp. 175–178, Pergamon Press/Akademiai Kiado, Budapest.

    Google Scholar 

  • Spitzer, J. J., Bechtel, A. A., Archer, L. T., Black, M. R., and Hinshaw, L. B., 1974, Myocardial substrate utilization in dogs following endotoxin administration, Am. J. Physiol. 227:132–136.

    PubMed  CAS  Google Scholar 

  • Spitzer, J. J., Ferguson, J. L., Hirsch, H. J., Loo, S., and Gabbay, K. W., 1980, Effects of E. coli endotoxin on pancreatic hormones and blood flow, Circ. Shock 7:353–360.

    PubMed  CAS  Google Scholar 

  • Vydelingum, N., Kissebah, A. H., and Wijn, V., 1978, Importance of cyclic guanosine 3′5′-monophosphate and calcium ions in insulin stimulation of lipoprotein lipase activity and protein synthesis in adipose tissue, Horm. Metab. Res. 10:38–46.

    Article  PubMed  CAS  Google Scholar 

  • Witek-Janusek, L., and Filkins, J. P., 1981, Insulin-like action of endotoxin: Antagonism by steroidal and non-steroidal anti-inflammatory agents, Circ. Shock 8:573–583.

    PubMed  CAS  Google Scholar 

  • Wolfe, R. R., Elahi, D., and Spitzer, J. J., 1977a, Glucose and lactate kinetics after endotoxin administration in dogs, Am. J. Physiol. 232:E180–E185.

    PubMed  CAS  Google Scholar 

  • Wolfe, R. R., Elahi, D., and Spitzer, J. J., 1977b, Glucose kinetics in dogs following a lethal dose of endotoxin, Metabolism 26:847–850.

    Article  PubMed  CAS  Google Scholar 

  • Woods, M. W., Burke, D., Howard, T., and Landy, M., 1961, Insulin-like action of endotoxins in normal and leukemic leukocytes and other tissues, Proc. Am. Assoc. Cancer Res. 3:279.

    Google Scholar 

  • Woods, M. W., Landy, M., Burke, D., and Howard, T., 1964, Effect of endotoxin on cellular metabolism, in: Bacterial Endotoxins (M. Landy and W. Braun, eds.), pp. 160–181. Rutgers University Press, New Brunswick, N.J.

    Google Scholar 

  • Zenser, T. V., DeRobertis, F. R., George, D. T., and Rayfield, E. J., 1974, Infection-induced hyperglucagonemia and altered hepatic response to glucagon in the rat, Am. J. Physiol. 227:1299–1305.

    PubMed  CAS  Google Scholar 

  • Zwadyk, P., Jr., and Snyder, L. S., 1973a, Effects of endotoxin on hepatic glycogen metabolism in vitro, Proc. Soc. Exp. Biol. Med. 142:299.

    PubMed  CAS  Google Scholar 

  • Zwadyk, P., Jr., and Snyder, L. S., 1973b, Effects of endotoxin on glycogenolytic enzymes of mouse liver, Proc. Soc. Exp. Biol. Med. 143:864–868.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Physiology, Louisiana State University Medical Center, New Orleans, Louisiana, 70112, USA

    Judy A. Spitzer & John J. Spitzer

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  1. Judy A. Spitzer
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  2. John J. Spitzer
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  1. Center for Oral Health Research, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA

    Alois Nowotny

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© 1983 Plenum Press, New York

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Spitzer, J.A., Spitzer, J.J. (1983). Effect of LPS on Carbohydrate and Lipid Metabolism. In: Nowotny, A. (eds) Beneficial Effects of Endotoxins. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4364-6_2

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  • DOI: https://doi.org/10.1007/978-1-4684-4364-6_2

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-4366-0

  • Online ISBN: 978-1-4684-4364-6

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