Abstract
Of the some 1500 chemicals used in agriculture (99), pesticides are the most controversial for their known or potential genotoxicity (mutagenicity-carcinogenicity-teratogenicity) and will comprise the subject matter of this paper. Pesticides are a large diverse group of compounds which include fungicides, herbicides, insecticides, fumigants, growth regulators and inhibitors, acaricides, rodenticides, soil nematicides, seed sterilants, soil conditioners, and chemosterilants. In addition, there are emulsifiers and solvents which are combined with pesticides and which are potential mutagens in their own right (25).
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References
Ahmed, M. and W.F. Grant, Cytological effects of the pesticides Phosdrin and Bladex on Tradescantia and Vicia faba, Can. J. Genet. Cytol. 14: 157–165 (1972).
Al-Najjar, N.R. and A.L. Soliman, Cytological effects of fungicides. I, Mitotic effects of vitavax-200 and dithane S-60 on wheat and two related species, Cytologia 45: 163–168 (1980).
Amer, S.M., and E.M. All, Cytоlogical effects of pesticides. IV. Mitotic effects of some phenols, Cytologia 34: 1–8 (1969).
Amer, S.M. and E.M. Ali, Cytological effects of pesticides, V. Effects on Vicia faba, Cytologia 39: 633–643 (1974)
Amer, S.M. and E.M. Ali, Cytological effects of pesticides. XI. Meiotic effects of the herbicides monochloracetic and trichloroacetic acids, Cytologia 45: 715–719 (1980).
Amer, S.M. and O.R. Farah, Cytological effects of pesticides. III. Meiotic effects of N-methyl-l-naphthyl carbamate “Sevin”, Cytologia 33: 337–344 (1968).
Amer, S.M. and O.R. Farah, Cytological effects of pesticides. VI. Effects of the insecticide “Rogor” on the mitosis of Vicia faba and Gossypium barbadense, Cytologia 39: 507–514 (1974).
Amer, S.M. and O.R. Farah, Cytological effects of pesticides. VIII. Effects of the carbamate pesticides “IPC”, “Rogor” and “Duphar” on Vicia faba, Cytologia 41: 597–606 (1976).
Amer, S.M. and O.R. Farah, Cytological effects of pesticides. IX. Effects of the phosphonothloate insecticide Leptophos on Vicia faba, Cytologia 44: 907–913 (1979).
Amer, S.M. and O.R. Farah, Cytological effects of pesticides X. Meiotic effects of “Phosvel”, Cytologia 45: 241–245 (1980).
Ames, B.N., Identifying environmental chemicals causing mutations and cancer, Science 204: 587–593 (1979).
Ashton, F.M., E.G. Cutter and D. Huffstutter, Growth and structural modifications of oats induced by bromacil, Weed Res. 9: 198–204 (1969).
Baquar, S.R. and N.R. Kahn, Effect of y-hexachloro- cyclohexane (HCCH) on the mitotic cells of Pisum sativum L., Rev. Biol. 7: 195–202 (1971).
Bandeen, J.D. and V.S. Machado, Weeds resist trlazine, Highlights of Agricultural Research in Ontario 1 (2): 1–2 (1978).
Bieleckl, E., The Influence of phenyl mercury acetate on mitosis and chromosome structure in Allium ceps, Crac. Ser. Bot. 17: 119–132 (1974).
Bilderback, D.E., Impatiens pollen germination and tube growth as a bloassay for toxic substances, Environ. Health Perspect. 37: 95–103 (1981).
Boyle, W.S., Cytogenic effects of Benlate fungicide on Allium cepa and Secale cereale, J. Hered. 64: 49–50 (1973).
Bradley, M.V., J.C. Crane, and N. Marei, Some histological effects of 2,4,5-trichlorophenoxyacetic acid applied to mature apricot leaves, Bot. Gaz (Chicago)129: 231–238 (1968)
Canvin, D.T., and G. Friesen, Cytological effects of CDAA and IPC on germinating barley and peas, Weeds 7: 153–156 (1959).
Chou, T.-S., and D.F. Weber, Visualization of sister- chromatid exchanges in maize mitotic chromosomes utilizing 5- bromodeoxyuridine. Maize Genet. Coop. Newslet. 54: 88 (1980).
Christianson, M.L. and M.O. Chiscon, Use of haploid plants as bioassays for mutagens, Environ. Health Perspect. 27: 77–83 (1978).
Conrad, S.G., and S.R. Radosevich, Ecological fitness of Senecio vulgaris and Amaranthus retroflexus biotypes susceptible or resistant to atrazine, J. Appl. Ecol. 16: 171–177 (1979).
Croker,B.H., Effects of 2,4-dichlorophenoxyacetic acid and 2,4,5-trichlorophenoxyacetic acid on mitosis in Allium cepa, Bot. Gaz. (Chicago) 114: 274–283 (1953).
Patino, J.F. Curtis, Efecto de dos pesticidas agricolas en el comportamiento mitotico de los cromosomas de hava (Vicia faba L.), Chapingo, Neuva Ecoca 16–17: 11–14 (1979).
Davring, L. and M. Sunner, Late prophase and first metaphase in female meiosis of Drosophila melanogaster, Heréditas 85: 25–32 (1977).
Derenne, P., Effets morphologiques, physiologiques et cytologiques dus xa l’action de l’isoprophylphenylcarbamate sur les genres Allium, Vicia et Hordeum, Bull. Inst. Agron. Stn. Rech. Gembloux 21: 37–57 (1953).
de Serres, F.J., Introduction: Utilization of higher plant systems as monitors of environmental mutagens. Environ. Health Perspect. 27: 3–6 (1978).
Doxey, D., The effect of isopropyl phenyl carbamate on mitosis in rye (Secale cereale) and onion (Allium cepa), Ann. Bot. 13: 329–335 (1949).
Ducruet, J.M. and J. Gasquez, Observation de la fluorescence sur feuille entière et mise en évidence de la résistance chloroplaetique à l’atrazine chez Chenopodium album et Poa annua L., Chemosphere 8: 691–696 (1978).
DuPraw, E.J., DNA and Chromosomes, Holt, Rinehart and Winston, New York (1970).
Dustin, P., Microtubules, Springer-Verlag, New York (1978).
Dvorak, J., Endopolyploidy in the roots of rye, Secale cereale L., Biol. Plant. 10: 112–117 (1968).
Ehrenberg, L., A. Gustafsson, A. Levan, and U. v. Wettstein, Radiophosphorus, seedling lethality and chromosome disturbances, Hereditas 35: 469–489 (1949).
Ennls, W. В., Jr., Some cytological effects of o-isopropyl-N- phenyl carbamate upon Avena, Am. J. Bot. 35: 15–21 (1949).
Femandez-Gomez, M. E., Alteraciones en el clclo de division celular inducidas por los isomeros del HCCH. I. Isomeros alfa, beta y gamma, Genet. Ibe. 19: 103–121 (1967).
Fernandez-Gomez, M.E., G. Gimenez-Martin, and J.F. Lopez-Saez, Alteraciones en el ciclo de division celular inducidas рог 1оs isomeros del HCCH. II. Isomero delta. Poslble mecanismo de acclon del HCCH, Genêt. Ibé. 19: 123–142 (1967).
FlskesjS, G., Some results from Allium tests with organic mercury halogenldes, Hereditas 62: 314–322 (1969).
Fiskeejô, G. Hie effect of two organic mercury compounds on human leukocytes In vitro, Heredltas 64: 142–146 (1970).
Frank, V., Restoration of mitotic and differentiation processes in the root apices of Allium cepa treated with cyanein and griseofulvin, Biol. Plant. 16: 28–34 (1974).
Freeling, M., Maize Adh 1 as a monitor of environmental mutagens, Environ. Health Perspect. 27: 91–97 (1978).
Gentner, W.A. and L.G. Burk, Gross morphological and cytological effects of nitralin on corn roots, Weed Sci. 16: 259–260 (1968).
Gibson, P.B. and G. Belnhart, Abnormal melosls in clover plants treated with organic phosphate pesticides, Bull. South Carolina Acad. Sci. 31: 38 (1969).
Gopalan, H.N.B. and G.D.E. Njagi, Cytogenetic studies in Vicia faba with some insecticides, fungicides and herbicides, Environ. Mutagen. 1: 141–142 (1979).
Gori, C. and E. Maugini, Effetti citplogici et rizogeni di alcune sostanze di crescita, Caryologia 7: 404–414 (1955).
Grant, W.F., Cytogenetic factors associated with the evolution of weeds, Taxon 16: 283–293 (1967).
Grant, W.F., Pesticides — subtle promoters of evolution, Symp. Biol. Hung. 12: 43–50 (1972).
Grant, W.F., Chromosome aberrations in plants as a monitoring system, Environ. Health Perspect. 27: 37–43 (1978).
Grant, W.F., The genotoxlc effects of 2,4,5-T, Mutat. Res. 65: 83–119 (1979).
Grant, W.F., Chromosome aberration assays in Allium, Mutat. Res. In press (1982).
Grant, W.F., A.E. Zinov’eva-Stahevitch, and K.D. Zura, Plant genetic test systems for the detection of chemical mutagens, in: “Short Term Tests for Chemical Carcinogens”, H.F. Stich and R.H.C. San, eds., Springer-Verlag, New York, pp. 200–216 (1981).
Gressel, J., Genetic herbicide resistance: Projections on appearance in weeds and breeding for it in crops, in: “Plant Regulation and World Agriculture”, T.K. Scott, ed., Plenum Press, New York (1979).
Grignac, P., The evolution of resistance to herbicides in weedy species, Agro-Ecosystems 4: 377–385 (1978).
Grover, I.S. and P. Kaur, personal communication (1981).
Grover, I.S. and P.S. Tyagi, Chromosomal aberrations induced by pesticides in meiotlc cells of barley, Caryologia 33: 251–260 (1980).
Guetafsson, A., The mutation system of the chlorophyll apparatus, Lunds Univ. Arsskr. 36: 1–40 (1940).
Heath, C.W., Jr., Environmental pollutants and the epidemiology of cancer, Environ. Health Perspect. 27: 7–10 (1978).
Herichova, A., Study of the effect of isopropyl-N-phenyl- carbamate and isopropyl-N-(3 chlorophenyl) carbamate on chromosome structure and cytokinesis, Acta F.R. N. Univ. Comen. Physiol. Plant. 1: 147–154 (1970).
Herichova, A., Cytological study on the effect of isopropyl- N-(3-chlorophenyl) carbamate on the division and ultra- structure of meristematic cells of barley (Hordeum sativum L. Acta F.R. N. Univ. Comen. Physiol. Plant. 6; 85–107 (1973).
Hervas, J.P. and G. Gimenez-Martin, Measurements of multipolar anaphases production by Y-hexachlorocyclohexane in onion root tip cells, Cytobiologie 9: 233–239 (1974).
Jagoda, M., Cytological disturbances in Allium cepa L. root-meristems induced by herbicides, Acta Biolog. Cracov. 22; 189–211 (1980).
Kabarity A. and A. Nahas, Induction of polypoloidy and C- tumors after treating Allium cepa with the herbicide ‘Treflan’, Biol. Plant. 21: 253–258 (1979).
Kar, S., Cytogenetic effects of 2,4-D on Pisum sativum, Proc. Indian Sci. Congr. Bot. 62: 122 (1975).
Kihlman, B.A., Root tips of Vicia faba for the study of the induction of chromosomal aberration, Mutat. Res. 31: 401–412.
Kihlman, B.A. and S. Sturelid, Effects of caffeine on the frequencies of chromosomal aberrations and sister chromatid exchanges induced by chemical mutagens in root tips of Vicia faba, Hereditas 88: 35–41 (1978).
Klâsterskâ, I., A.T. Natarajan and C. Ramel, An interpretation of the origin of subchromatid aberrations and chromosome stickiness as a category of chromatid aberrations, Hereditas 83: 153–162 (1976).
Klekowski, E.J., Jr., Detection of mutational damage in fern populations: An in situ bioaesay for mutagens in aquatic ecosystems, in: “Chemical mutagens. Principles and Methods for their Detection”, vol. 5, A. Hollaender and J.F. de Serres, eds., Plenum Press, New York, pp. 79–99 (1978).
Rostoff, D., Heteroploidy in Nicotiana tabacum and Solanum melongena caused by fumigation with nicotine sulfate, Bull. Soc. Bot. Bulgar. 4: 87–92. (Biol. Abstr. 8: 10, 1934) (1931).
Levan, A., The effect of colchicine on root mitosis in Allium, Hereditas 24: 471–486 (1938).
Levan, A., The influence on chromosomes and mitosis of chemicals, as studied by the Allium test. Proc. 8th Int. Congr. Genet., Stockholm, Hereditas, Suppl. 325–337 (1949).
Levan, A., Chemically induced chromosome reactions in Allium cepa and Vicia faba, Cold Spring Harbor Symp. Quant. Biol. 16: 233–244 (1951).
Liang, G.H. and Y.T.S. Liang, Effects of atrazine on chromosomal behavior in sorghum, Can. J. Genet. Cytol. 14: 423–427 (1972).
Lower, W.R., P.S. Rose and V.K. Drobney, In situ mutagenic and other effects associated with lead smelting, Mutat. Res, 54: 83–93 (1978).
Ma, T.-H., Tradescantia micronucleus bioassay and pollen tube chromatid aberration test for in situ monitoring and mutagen screening. Environ. Health Perspect. 27: 85–90 (1981).
Mann, J.D. and W.B. Storey, Rapid action of carbamate herbicides upon plant cell nuclei, Cytologia 31: 203–207 (1966).
Mann, J.D., L.S. Jordan, and B.E. Day, The effects of carbamate herbicides on polymer synthesis, Weeds13: 63–66 (1965).
Mann, S.K., Cytоlogical and genetlcal effects of dithane fungicides on Allium cepa, Environ. Exp. Bot. 17: 7–12 (1977).
Markaryan, D.S., Effect of dieldrin on the mitosis in Crepis capillaris sprouts, Genetika 3: 55–58 (1967).
McGill, M.S. Pathak, and T.C. Hsu, Effects of ethidium bromide on mitosis and chromosomes: A possible material basis for chromosome stickiness, Chromosoma 47: 157–167 (1974).
McLeish, J., The action of maleic hydrazide in Vicia, Heredity (Suppl.) 6: 125–147 (1953).
Mohandas, T and W.F. Grant, Cytogenetic effects of 2,4-D and amitrole in relation to nuclear volume and DNA content in some higher plants, Can. J. Genet. Cytol. 14: 773–783 (1972).
Mohandas, T. and W.F. Grant, A relationship between nuclear volume and response to auxin herbicides for some weed species, Can. J. Bot. 51: 1133–1136 (1973).
Morrison, J.W., Cytological effects of the herbicide “Avadex”, Can. J. Plant. Sci Can. J. Plant. Sci. 42: 78–81 (1962).
Miühling, G.N., J. Van’t Hof, G.B. Wilson, and B.H. Grigsby, Cytological effects of herbicidal substituted phenols, Weeds, 8: 173–181 (1960).
Mulcahy, D.L., Pollen tetrads in the detection of environmental mutagenesis, Environ. Health Perspect. 37: 91–94 (1981).
Mulcahy, D.L. and C.M. Johnson, Self-incompatibility systems as bloassays for mutagens, Environ. Health Perspect. 27: 85–90 (1978).
Nicoloff, H. and K. Gecheff, Methods of scoring induced chromosome structural changes in barley, Mutat. Res. 34: 233–244 (1976).
Nilan, R.A., J.L. Rosichan, P. Arenaz, A.L. Hodgdon, and A. Kleinhofs, Pollen genetic markers for detection of mutagens in the environment, Environ. Health Perspect. 37: 19–25 (1981).
Njagi, G.D.E. and H.N.B. Gopalan, Mutagenicity testing of herbicides, fungicides and insecticides. 1. Chromosome aberrations in Vicia faba, Cytologia 46: 169–172 (1981).
Nygren, A., Cytological studies of the effects of 2,4-D, MCPA, and 2,4,5-T on Allium cepa, Ann. Roy. Agric. Coll, Sweden 16: 723–728 (1949).
Paget, G.E. and A.L. Walpole, Some cytological effects of griseofulvin, Nature (London) 182; 1320–1321 (1958).
Panda, B.B. and C.B.S.R. Sharma, Organophosphate induced chlorophyll mutations in Hordeum vulgare, Theor. Appl. Genet. 55: 253–255 (1979).
Panda, B.B. and C.B.S.R. Sharma, Cytogenetic hazards from agricultural chemicals 3. Monitoring the cytogenetic activity of trichlorfon and dichlorvos in Hordeum vulgare, Mutat. Res. 78: 341–345 (1980).
Panda, B.B., B.W. Behera, and C.B.S.R. Sharma, Mutagenic potential of some insecticides and fungicides in plant systems, in: Nuclear techniques in studies of metabolism effects and degradation of pesticides. Proc. Symp. at Sri Venkateswara Univ. Tirupati, Dept. of Atomic Energy, Govt, of India, pp. 190–203 (1978).
Plewa, M.J. and E.D. Wagner, Germinal cell mutagenesis in specially designed maize genotypes, Environ. Health Perspect. 37: 61–73 (1981).
Prasad, I. and D. Pramer, Genetics effects of ferbam on Aspergillus niger and Allium cepa, Phytophathology 58: 1188–1189 (1968).
Price, M. and S.C. Schank, Chromosomal damage and abnormal seedling development in barley induced by chemical treatment with TIBA, maleic hydrazide and foramide, Proc. Soil and Crop Sci. Soc. Fla. 32: 41–46, Agron. Dept. U. Fla. Gainesville (1973).
Pueztia, T. and A. Végh, Mutagenic effects of pesticides. I. Acta Bot. Acad. Sci. Hung. 24: 327–342.
Rédei, G.P. and G. Acedo, Biochemical mutants in higher plants, in: “Cell Genetics in Higher Plants”, D. Dudits, G.L. Farkas and P. Maliga, eds., Akademiai Kaido, Budapest, p. 39 (1976).
Ridgway, R.L., J.C. Tinney, J.T. MacGregor and N.J. Star1er, Pesticide use in agriculture, Environ. Health Perspect. 27; 103–112 (1978).
Sandhu, S.S., Regulation of environmental pollutants: Introductory remarks, Environ. Health Perspect. 1–3 (1981).
Sawamura, S., Cytological studies on the effect of herbicides on plant cells in vivo II. Non-hormonic herbicides, Cytologia 30: 325–348 (1965).
Schairer, L.A., J. Van’t Hof, C.G. Hayes, R.M. Burton, and F.J. de Serres, Exploratory monitoring of air pollutants for mutagenicity activity with the Tradescantla stamen hair system. Environ. Health Perspect. 27: 51–60 (1978).
Schairer, L.A., A.H. Sparrow, and N.R. Tempel, Mobile monitoring vehicle designed to assess the mutagenicity of ambient air in high pollution areas, Mutation Res. 53: 111–112 (1978).
Scholes, M.E., The effects of aldrin, dieldrin, isodrin, endrin and DDT on mitosis in roots of the onion (Allium cepa L.), J, Hortic. Sci. 30: 181–187 (1955).
Schubert, I., G. K. Unzel, H. BretSchneider, R. Rieger, and H. Nicoloff, Sister chromatid exchanges in barley, Theoret. Appl. Genet. 56; 1–4 (1980).
Schvartzman, J.B. and P. Hernandez, Sister chromatid exchanges and chromosomal aberrations in 5-aminouracil-synchronlzed cells, Theor. Appl. Genet. 57: 221–224 (1980).
Schwartz, D., Adh locus in maize for detection of mutagens in the environment, Environ. Health Perspect. 37: 75–77 (1981).
Sharma, A.K. and S. Gosh, A comparative study on the effects of certain chemical agents on chromosomes, Acta Biol. Acad. Sci. Hung. 20: 11–21 (1969).
Sharma, C.B.S.R., B. Partra, D.S.S. Raju and K.V. Murty, Chlasma variation in Hordeum vulgare after exposure to herbicides, Mutation Res. 91: 333–336 (1981).
Shelby, M.D. and the Environmental Mutagen Information Center Staff, “Chemical Mutagenesis in Plants and Mutagenicity of Plant Related Compounds”, Oak Ridge National Laboratory, ORNL/EMIC-7 (1976).
Singh, B.D., T.B. Singh, R.M. Singh, Y. Singh and J. Singh, Effect of insecticides on germination, early growth and cytogenetic behavior of barley (Hordeum vulgare), Environ. Exp. Bot. 19: 127–132 (1979).
Singh, R.M., A.K. Singh, R.B. Singh, J. Singh, and B.D. Singh, Chlorophyll mutations induced by seed treatment with certain insecticides in barley (Hordeum vulgare), Indian J. Exp. Biol. 18: 1396–1397 (1980).
Soliman, A.S. and N.R. Al-Najjar, Cytological effects of fungicides. II. Chromosomal aberrations induced by vitavax- 200 and dithane S-60 In meiotic cells of wheat and two related species, Cytologla 45: 169–175 (1980).
Spasojevic, V., Effect of fungicide Benlate on the mitosis of maize, Arch. Poljopr. Nanke 27 (100): 13–21 (1974).
Spasojevic, V., Cytogenetlcal effect of Tuberite on Zea mays, Arkiv. Poljopr. Nanke 28: 119–125 (1975).
Ramulu, K. Sree, H. Schibilla, and P. Dijkhuis, Self-incompatibility system of Oenothera organesls for the detection of genetic effects at low radiation doses, Environ. Health Perspect. 37: 43–51 (1981).
Sterrett, R.B. and T.A. Fretz, Asulam-induced mitotic Irregularities in onion root-tips, Hortscience 10: 161–162 (1975).
Storey, W.B.., L.S. Jordan and J.D. Mann, Carbamate herbicides — new tools for cytological studies, Calif. Agric. 22 (8): 12–13 (1968).
Suneson, C.A. and L.G. Jones, Herbicides may produce instability, Agron. J. 52: 120–121 (1960).
Suneson, C.A., H.C. Murphy, and F.C. Petr, Fostering the recombination of oats with a mutagen, Crop Sci. 5: 176 (1965).
Tomkins, D.J. and W.F. Grant, Monitoring natural vegetation for herbicide-induced chromosomal aberrations. Mutat. Res. 36: 73–84 (1976).
Unrau, J., Cytogenetic effects of 2,4-D on cereals, Annu. Rep. Can. Seed Growers Assoc. 1953–1954; 37–39 (1954).
Unrau, J. and E.N. Barter, Cytogenetical reponse of cereals to 2,4-D. I. A study of meiosis of plants treated at various stages of growth, Can. J. Bot. 30: 22–27 (1952).
Vaarama, A., Experimental studies on the influence of DDT pesticide upon plant mitosis, Hereditas 33: 191–219 (1947).
Vig, B.K., Somatic mosaicism in plants with special reference to somatic crossing over. Environ. Health Perspect. 27: 27–36 (1978)
Warwick, S.I., V. Souza Machado, P. B. Marriage, and J. D. Bandeen, Resistance of Chenopodium strictumRoth (late- flowering goosefoot) to atrazine, Can. J. Plant Sci. 59: 269–270 (1979).
Wuu, K.D. and W.F. Grant, Morphological and somatic chromosomal aberrations induced by pesticides in barley (Hordeum vulgare), Can. J. Genet. Cytol. 8: 481–501 (1966).
Wuu, K.D. and W.F. Grant, Induced abnormal meiotic behavior in a barley plant (Hordeum vulgare L.) with the herbicide Lorox, Phyton, 23: 63–67 (1966).
Wuu, K.D. and W.F. Grant, Chromosomal aberrations induced by pesticides in meiotic cells of barley, Cytologia 32: 31–41 (1967).
Wuu, K.D. and W.F. Grant, Chromosomal aberrations induced in somatic cells of Vicia faba by pesticides, Nucleus 10: 37–46 (1967).
Yakar, N., Mitotic disturbances caused by chloranil, Am. J. Bot. 39: 540–546 (1952).
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Grant, W.F. (1982). Cytogenetic Studies of Agricultural Chemicals in Plants. In: Fleck, R.A., Hollaender, A. (eds) Genetic Toxicology. Basic Life Sciences, vol 6. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4352-3_25
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