Abstract
DR. LOUIS M. KUNKEL: Dr. Latt has described some of the work currently in progress in our laboratory but has left to me the task of describing our work related to flow sorting and cloning the human X chromosome. The goal was to obtain a library of recombinant phage highly enriched for X chromosome DNA sequences. These phage would serve as sources of DNA possibly useful in linkage analysis with known X-linked loci or in the acquisition of the more than 100 known X-linked genes (52). I would like to describe briefly the construction of the X chromosome enriched library and our initial characterization of some of the human inserts from this library. Lastly, I would like to describe our initial efforts to obtain sequences of DNA which might possibly encode for X-linked proteins.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1982 Plenum Press, New York
About this chapter
Cite this chapter
Epstein, H.F., Wolf, S. (1982). DNA Probes: Development of Libraries. In: Epstein, H.F., Wolf, S. (eds) Genetic Analysis of the X Chromosome. Advances in Experimental Medicine and Biology, vol 154. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4346-2_3
Download citation
DOI: https://doi.org/10.1007/978-1-4684-4346-2_3
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4348-6
Online ISBN: 978-1-4684-4346-2
eBook Packages: Springer Book Archive