Abstract
Advances in molecular biology and methods in mammalian cell culture have been utilized in the construction of DNA-transformed, pluripotent murine teratocarcinoma stem cells (Linnenbach et al., 1980) that have proven to be a useful in vitro model for the study of the regulation of gene expression during differentiation (Huebner et al., 1981; Croce et al., 1981; Linnenbach et al., 1981).
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References
Alwine, J. C., Kemp, D. J., Parker, B. A., Reiser, J., Renart, J., Stark, G. A., and Wahl, G. M., 1979, Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper, in: Methods in Enzymology, Volume 68 (R. Wu, ed.), Academic Press, New York, pp. 220–242.
Aviv, H., and Leder, P., 1972, Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose, Proc. Natl. Acad. Sci. USA 69:1408–1412.
Bantle, J. A., Maxwell, I. H., and Hahn, W. E., 1976, Specificity of oligo (dT)-cellulose chromatography in the isolation of polyadenylated RNA, Anal. Biochem. 72:413–427.
Berger, S., and Birkenmeir, C., 1979, Inhibition of intractable nucleases with ribonucleosidevanadyl complexes: Isolation of messenger ribonucleic acid from resting lymphocytes, Biochemistry 18:5143–5149.
Berk, A. J., and Sharp, P. A., 1977, Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of Si endonuclease digested hybrids, Cell 12:721–732.
Berk, A. J., and Sharp, P. A., 1978, Spliced early mRNAs of simian virus 40, Proc. Natl. Acad. Sci. USA 75:1274–1278.
Bolivar, F., Rodriguez, R., Green, P., Betlach, M., Heyneker, H., Boyer, H., Crosa, I., and Falkow, S., 1977, Construction and characterization of new cloning vehicles. II. A multi-purpose cloning system, Gene 2:95–113.
Carmichael, G., and McMaster, G., 1980, The analysis of nucleic acids in gels using glyoxal and acridine orange, in: Methods in Enzymology, Volume 65 (L. Grossman and K. Maldowe, eds.), Academic Press, New York, pp. 380–391.
Clewell, D. E., and Helsinki, D. R., 1960, Supercoiled DNA-protein complex in Escherichia Coli: Purification and induced conversion to an open circular DNA form, Proc. Natl. Acad. Sci. USA 62:1159–1166.
Croce, C. M., Linnenbach, A., Huebner, K., Parnes, J., Margulies, D., Appela, E., Seidman, J., 1981, Control of expression of histocompatibility antigens (H-2) and β2-microglobulin in F9 teratocarcinoma stem cells, Proc. Natl. Acad. Sci. USA 78:5754–5758.
Curtiss, R., III, Pereira, D. A., Hsu, J. C., Hull, S. C., Clark, J. F., Maturin, L. J., Sr., Goldschmidt, R., Moody, R., Inoue, M., and Alexander, L., 1977, Biological containment: The subordination of Escherichia coli K-12, in: Recombinant Molecules: Impact on Science and Society (R. F. Beers and E. G. Bassett, eds.), Raven Press, New York, pp. 45–56.
Duesberg, P., and Vogt, P., 1973, Gel electrophoresis of avian leukosis and sarcoma viral RNA in formamide: Comparison with the other viral and cellular RNA species, J. Virol. 12:594–599.
Favalora, J., Triesman, R., and Karmen, R., 1980, Transcriptional maps of polyoma virus-specific RNA: Analysis by two-dimensional nuclease S1 gel mapping, in: Methods in Enzymology, Volume 65 (L. Grossman and K. Maldowe, eds.), Academic Press, New York, pp. 718–749.
Glisin, V., Crkvenjakov, R., and Byus, C., 1974, Ribonucleic acid isolation by cesium chloride centrifugation, Biochemistry 13:2633–2637.
Gmiir, R., Solter, D., and Knowles, B. B., 1980, Independent regulation of H-2K and H-2D gene expression in murine teratocarcinoma somatic cell hybrids, J. Exp. Med. 151:1349–1359.
Graham, F.L., and Van der Eb., A. J., 1973, Anew technique for the assay of infectivity of human adenovirus 5 DNA, Virology 52:456–567.
Grunstein, M., and Hogness, D. S., 1975, Colony hybridization: A method for the isolation of cloned DNAs that contain a specific gene, Proc. Natl. Acad. Sci. USA 72:3961–3965.
Hirt, B., 1967, Selective extraction of polyoma DNA from infected mouse cell cultures, J. Mol. Biol. 26:365–369.
Huebner, K., Linnenbach, A., Weidner, S., Glenn, G., and Croce, C. M., 1981, Deoxyribonuclease I sensitivity of plasmid genomes in teratocarcinoma-derived stem and differentiated cells, Proc. Natl Acad. Sci. USA 78:5071–5075.
Hughes, E. W., and August, T. T., 1981, Characterization of plasma membrane proteins identified by monoclonal antibodies, J. Biol. Chem. 256:664–671.
Knowles, B. B., Pan, S. H., Solter, D., Linnenbach, A., Croce, C. M., and Huebner, K., 1980, Expression of H-2 laminin and SV40T and TASA on differentiation of transformed murine teratocarcinoma cells, Nature 288:615–618.
Laemmli, U. K., 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227:680–685.
Linnenbach, A., Huebner, K., and Croce, C. M., 1980, DNA-transformed murine teratocarcinoma cells: Regulation of expression of simian virus 40 tumor antigen in stem versus differentiated cells, Proc. Natl Acad. Sci. USA 77:4875–4879.
Linnenbach, A., Huebner, K., and Croce, C. M., 1981, Transcription of the simian virus 40 genome in DNA-transformed murine teratocarcinoma stem cells, Proc. Natl Acad. Sci. USA 78:6386–6390.
Linzer, D. I., and Levine, A. I., 1979, Characterization of a 54K Dalton cellular SV40 tumor antigen present in SV40 transformed cells and uninfected embryonal carcinoma cells, Cell 17:43–52.
Littlefield, J. W., 1965, The use of drug-resistant markers to study the hybridization of mouse fibroblasts, Exp. Cell Res. 41:190–196.
Maniatis, T., Kee, S. G., Efstratiadis, A., and Katatos, F. C., 1976, Amplification and characterization of a β-globin gene synthesized in vitro, Cell 8:163–182.
Martin, G., and Evans, M. J., 1975, Multiple differentiation of clonal teratocarcinoma stem cells following embryonal body formation in vitro, Cell 6:467–474.
Martinis, J., and Croce, C. M., 1978, Somatic cell hybrids producing antibodies specific for the tumor antigen of simian virus 40, Proc. Natl Acad. Sci. USA 75:2320–2323.
Maxam, A. M., and Gilbert, W., 1977, A new method for sequencing DNA, Proc. Natl Acad. Sci. USA 74:560–564.
McKnight, S., 1980, The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene, Nucleic Acids Res. 8:5949–5964.
McKnight, S., Croce, C. M., and Kingsbury, R., 1979, Introduction of isolated DNA sequences into cultured eukaryotic cells, Carnegie Institute Yearbook 78:56–61.
Migeon, B., Smith, S. W., and Leddy, C., 1969, The nature of thymidine kinase in the humanmouse hybrid cell, Biochem. Genet. 3:583–590.
Pope, J. H., and Rowe, W. P., 1964, Detection of specific antigen in SV40 transformed cells by immunofluorescence, J. Exp. Med. 120:121–127.
Radloff, R., Bauer, W., and Vinograd, J., 1967, A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: The closed circular DNA in Hela cells, Proc. Natl. Acad. Sci. USA 57:1514–1522.
Scherrer, K., 1969, Isolation and sucrose gradient analysis of RNA, in: Fundamental Techniques in Virology (K. Habel and N. Salzman, eds.), Academic Press, New York, pp. 413–432.
Segal, S., and Khoury, G., 1979, Differentiation as a requirement for simian virus 40 gene expression in F9 embryonal carcinoma cells, Proc. Natl. Acad. Sci. USA 76:5611–5616.
Southern, E., 1975, Detection of specific sequences among DNA fragments separated by gel electrophoresis, J. Mol. Biol. 98:503–517.
Spencer, N., Hopkinson, D. A., and Harris, H., 1964, Phosphoglucomutase polymorphism in man, Nature 204:742–745.
Strickland, S., and Mahdavi, V., 1978, The induction of differentiation in teratocarcinoma stem cells by retinoic acid, Cell 15:393–403.
Thomas, P., 1980, Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose, Proc. Natl. Acad. Sci. USA 77:5201–5205.
Tonegawa, S., Brack, C., Hozumi, N., and Schuller, R., 1977, Cloning of an immunoglobulin variable region gene from mouse embryo, Proc. Natl. Acad. Sci. USA 74:3518–3522.
Wigler, M., Silverstein, S., Lee, L., Pellicer, A., Cheng, Y., Axel, R., 1977, Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells, Cell 11:223–232.
Wigler, M., Pellicer, A., Silverstein, S., Axel, R., Urlaub, G., and Chasin, L., 1979, DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells, Proc. Natl. Acad. Sci. USA 75:1373–1375.
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© 1982 Plenum Press, New York
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Linnenbach, A., Croce, C.M. (1982). Viral-DNA Vectors in the Analysis of Mammalian Differentiation. In: Shay, J.W. (eds) Techniques in Somatic Cell Genetics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4271-7_30
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DOI: https://doi.org/10.1007/978-1-4684-4271-7_30
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