Abstract
DNA transformation in bacteria has permitted detailed genetic analysis as well as the implementation of recombinant DNA technology. The utility of manipulation and cloning of genes in bacteria is well recognized. This chapter will concern the manipulation and cloning of genes in eukaryotic systems ranging from yeast to animal systems. Gene manipulation utilizing eukaryotic cloning systems permits the analysis of regulation of eukaryotic genes in their native environment and offers certain advantages for the production of foreign gene products which may require post-transcriptional modifications which are indigenous to eukaryotic organisms.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Beggs, J. D., 1978, Transformation of yeast by a replicating hybrid plasmid, Nature 275:104–109.
Bolivar, F., Rodriquez, R., Green, P., Betlach, M., Heyneka, H., and Boyer, H., 1977, Construction and characterization of new cloning vehicles II. A multipurpose cloning system, Gene 2:95–113.
Bollon, A. P., and Stauver, M., 1980, DNA transformation efficiency of various bacterial and yeast host-vector systems, J. Clin. Hematol Oncol. 10:39–48.
Broach, J. R., Strathern, J. N., and Hicks, J. B., 1979, Transformation in yeast: Development of a hybrid cloning vector and isolation of the CAN 1 gene, Gene 8:121–133.
Flintoff, W. F., Davidson, S. V., and Siminovitch, L., 1976, Isolation and partial characterization of three methotrexate-resistant phenotypes from Chinese hamster ovary cells, Somat. Cell Genet. 2:245–261.
Gordon, J. W., Scangos, G. A., Plotkin, D. J., Barbosa, J. A., and Ruddle, F. H., 1980, Genetic transformation of mouse embryos by microinjection of purified DNA, Proc. Natl. Acad. Sci. USA 77:7380–7384.
Graham, F. L., Abrahams, P. J., Mulder, C., Heijneker, H. L., Warnaar, S. O., de Vries, F. A. J., Fiers, W., and van der Eb, A. J., 1974, Studies on in vitro transformation by DNA and DNA fragments of human adenoviruses and Simian Virus 40, Cold Spring Harbor Symp. Quant. Biol. 39:637–650.
Hinnen, A., Hicks, J. B., and Fink, G. R., 1978, Transformation of yeast, Proc. Natl. Acad. Sci. USA 75:1929–1933.
Hitzeman, R. A., Hagie, F. E., Levine, H. L., Goeddel, D., Ammerer, G., and Hall, B. D., 1981, Expression of human gene for Interferon in yeast, Nature 293:717–722.
Hohn, B., and Hinnen, A., 1980, Cloning with cosmids in E. coli and yeast, in: Genetic Engineering, Volume 2 (J. K. Setlow and A. Hollaender, eds.), Plenum Press, New York, pp. 169–183.
Ilgen, C., Farabaugh, P. J., Hinnen, A., Walsh, J. A., and Fink, G. R., 1979, Transformation of yeast, in: Genetic Engineering, Volume 1 (J. K. Setlow and A. Hollaender, eds.), Plenum Press, New York, pp. 117–132.
Lowy, I., Pellicer, A., Jackson, J. F., Sim, G. K., Silverstein, S., and Axel, R., 1980, Isolation of transforming DNA: Cloning the hamster aprt gene, Cell 22:817–823.
McCutchan, J. H., and Pagano, J. S., 1968, Enhancement of infection of Simian Virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran, J. Natl. Cancer Inst. 41:351–356.
Mulligan, R. C., and Berg, P., 1980, Expression of a bacterial gene in mammalian cells, Science 209:1422–1427.
Perucho, M., Hanahan, D., Lipsick, L., and Wigler, M., 1980, Isolation of the chicken thymidine kinase gene by plasmid rescue, Nature 285:207–210.
Ratzkin, B., and Carbon, J., 1977, Functional expression of cloned yeast DNA in Escherichia coli, Proc. Natl. Acad. Sci. USA 74:487–491.
Robins, D., Ripley, S., Henderson, A., and Axel, R., 1981, Transforming DNA integrates into the host chromosome, Cell 23:29–39.
Scherer, S., and Davis, R. W., 1979, Replacement of chromosomal segments with altered DNA sequences constructed in vitro, Proc. Natl. Acad. Sci. USA 76:4951–4955.
Simm, G. K., and Augustin, A., 1981, Transfection of L-cells with cloned H-2 genes leads to expression of corresponding alloantigens, Nature (in press).
Stinchcomb, D. T., Struhl, K., and Davis, R. W., 1979, Isolation and characterization of a yeast chromosomal replicator, Nature 282:39–43.
Struhl, K., Cameron, J. R., and Davis, R. W., 1976, Functional genetic expression of eukaryotic DNA in Escherichia coli, Proc. Natl. Acad. Sci. USA 73:1471–1475.
Struhl, K., Stinchcomb, D. T., Scherer, S., and Davis, R. W., 1979, High frequency transformation of yeast: Autonomous replication of hybrid DNA molecules, Proc. Natl. Acad. Sci. USA 76:1035–1039.
Sweet, R., Jackson, J., Lowy, I., Ostrander, M., Pellicer, A., Roberts, J., Robins, D., Sim, G.-K., Wold, B., Axel, R., and Silverstein, S., 1981, The expression, arrangement, and rearrangement of genes in DNA-transformed cells, in: Genes Chromosomes and Neoplasia (F. E. Arrighi, P. N. Rao and E. Stubblefield, eds.), Raven Press, New York, pp. 205–219.
Szybalska, E. H., and Szybalski, W., 1962, Genetics of human cell lines IV. DNA-mediated heritable transformation of a biochemical trait, Proc. Natl. Acad. Sci. USA 48:2026–2034.
Wagner, E. F., Stewart, T. A., and Mintz, B., 1981, The human β-globin gene and a functional viral thymidine kinase gene in developing mice, Proc. Natl. Acad. Sci. USA 78:5016–5020.
Whelan, W. L., Gocke, E., and Manney, T. R., 1979, The CAN 1 locus of Sacchromyces cerevisiae: Fine structure analysis and forward mutation rates, Genetics 91:35–51.
Wigler, M., Sweet, R., Sim, G.-K., Wold, B., Pellicer, A., Lacy, E., Maniatis, T., Silverstein, S., and Axel, R., 1979, Transformation of mammalian cells with genes from prokaryotes and eukaryotes, Cell 16:777–785.
Wigler, M., Perucho, M., Kurtz, D., Dana, S., Pellicer, A., Axel, R., and Silverstein, S., 1980, Transformation of mammalian cells with an amplifiable dominant acting gene, Proc. Natl. Acad. Sci. USA 77:3567–3570.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1982 Plenum Press, New York
About this chapter
Cite this chapter
Bollon, A.P., Silverstein, S.J. (1982). Techniques of DNA-Mediated Gene Transfer for Eukaryotic Cells. In: Shay, J.W. (eds) Techniques in Somatic Cell Genetics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4271-7_29
Download citation
DOI: https://doi.org/10.1007/978-1-4684-4271-7_29
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4273-1
Online ISBN: 978-1-4684-4271-7
eBook Packages: Springer Book Archive