Abstract
The physical and chemical nature of chromosomes and the adherence of contaminating cellular materials under isolation conditions are important factors to be considered in selection of a method for mass isolation of chromosomes. The application for which the chromosomes are to be used may ultimately determine the procedure chosen.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Adolph, K. W., 1980, Isolation and structural organization of human mitotic chromosomes, Chromosoma 76:23–33.
Blumenthal, A. B., Dieden, J. D., Kapp, L. N., and Sedat, J. W., 1979, Rapid isolation of metaphase chromosomes containing high molecular weight DNA, J. Cell Biol. 81:255–259.
Burkholder, G. D., and Mukherjee, B. R., 1970, Uptake of isolated metaphase chromosomes by mammalian cells in vitro, Exp. Cell Res. 61:413–422.
Cantor, K. P., and Hearst, J. E., 1966, Isolation and partial characterization of metaphase chromosomes of a mouse ascites tumor, Proc. Natl. Acad. Sci. USA 55:642–649.
Carrano, A. V., Gray, J. W., Moore II, D. H., Minkler, J. L., Mayall, B. H., Van Dilla, M. A., and Mendelsohn, M. L., 1976, Purification of the chromosomes of the Indian muntjak by flow sorting, J. Histochem. Cytochem. 24:348–354.
Chorazy, M., Bendich, A., Borenfreund, E., and Hutchison, D. J., 1963, Studies on the isolation of metaphase chromosomes, J. Cell Biol. 19:59–69.
Cleveland, D. W., Hughes, H. E., Stubblefield, E., Kirchner, M. W., and Varmus, H. E., 1981, Multiple and tubulin genes represent unlinked and dispersed gene families, J. Biol. Chem. 256:3130–3134.
Deisseroth, A., Nienhuis, A., Turner, P., Velez, R., Anderson, W. F., Ruddle, F., Lawrence, J., Creagan, R., and Kucherlapati, R., 1977, Localization of the humana globin structural gene to chromosome 16 in somatic cell hybrids by molecular hybridization assay, Cell 12:205–218.
Franceschini, P., and Giacomoni, D., 1967, Isolation and fractionation of metaphase chromosomes from HeLa cells, Atti Assoc. Genet. Ital. 12:248–258.
Frenster, J. H., 1963, Constraints on isolation of mammalian chromosomes, Exp. Cell Res. Suppl. 9:235–238.
Goyanes, V. J., Matsui, S.-L, and Sandberg, A. A., 1980, The basis of chromatin fiber assembly within chromosomes studied by histone-DNA crosslinking followed by trypsin digestion, Chromosoma 78:123–135.
Gray, J. W., Carrano, A. V., Steinmetz, L. L., Van Dilla, M. A., Moore, D. H., Mayall, B. H., and Mendelsohn, M. L., 1975, Chromosome measurement and sorting by flow systems, Proc. Natl. Acad. Sci. USA 72:1231–1234.
Hamilton, M. G., and Peterman, M. L., 1959, Ultracentrifugal studies on ribonucleoprotein from rat liver microsomes, J. Biol. Chem. 234:1441–1446.
Hearst, J. E., and Botchan, M., 1970, The eukaryotic chromosome, in: Annual Review of Biochemistry, pp. 151-182, Annual Review Inc., Palo Alto, California.
Horan, P. K., and Wheeless, L. L., 1977, Quantitative single cell analysis and sorting, Science 198:149–157.
Hsu, T. C., 1973, Longitudinal differentiation of chromosomes, Ann. Rev. Genet. 7:153–156.
Huberman, J. A., and Attardi, G. J., 1966, Isolation of metaphase chromosomes from HeLa cells, J. Cell Biol. 31:95–105.
Huberman, J. A., and Attardi, G. J., 1967, Studies of fractionated HeLa cell metaphase chromosomes. I. The chromosomal distribution of DNA complementary to 28S and 18S ribosomal RNA and to cytoplasmic messenger RNA, J. Mol. Biol. 29:487–505.
Hughes, S. H., Stubblefield, E., Payvar, F., Engel, J. D., Dodgson, J. B., Spector, D., Cordell, B., Schimke, R. T., and Varmus, H. E., 1979, Gene localization by chromosome fractionation: Globin genes are on at least two chromosomes and three estrogen-inducible genes are on three chromosomes, Proc. Natl. Acad. Sci. USA 76:348–1352.
Inoue, S., 1964, Organization and function of the mitotic spindle, in: Primitive Motile Systems in Cell Biology (R. D. Allen and N. Kamiya, eds.), Academic Press, New York, pp. 549–598.
Kane, R. E., 1965, The mitotic apparatus. Physical-chemical factors controlling stability, J. Cell Biol. 25 (Suppl.):136–144.
Krajca, J. B., and Wray, W., 1977, Banding isolated metaphase chromosomes by a sequential fluorescent G/Q technique, Histochemistry 51:103–111.
Langlois, R. G., Carrano, A. V., Gray, J. W., and Van Dilla, M. A., 1980, Cytochemical studies of metaphase chromosomes by flow cytometry, Chromosoma 77:229–251.
Maio, J. J., and Schildkraut, C. L., 1967, Isolated mammalian metaphase chromosomes. I. General characteristics of nucleic acids and proteins, J. Mol. Biol. 24:29–39.
Mendelsohn, J., Moore, D. E., and Salzman, N. P., 1968, Separation of isolated Chinese hamster metaphase chromosomes into three size groups, J. Mol. Biol. 32:101–112.
Nikolayenko, N. S., and Pinaeu, G. P., 1981, Lipid composition of metaphase chromosomes dependent on the method of their isolation, Tsitologiya 23:185–192.
Padget, T. G., Stubblefield, E., and Varmus, H. E., 1977, Chicken macrochromosomes contain an endogenous provirus and microchromosomes contain sequences related to the transforming gene of ASV, Cell 10:649–657.
Peterson, J. L., and McConkey, E. H., 1976, Non-histone chromosomal proteins from HeLa cells. A survey by high resolution, two dimensional electrophoresis, J. Biol. Chem. 251:548–554.
Prescott, D. M., and Bender, M. A., 1961, Preparation of mammalian metaphase chromosomes free of cytoplasm, Exp. Cell Res. 25:222–223.
Somers, C. E., Cole, A., and Hsu, T. C., 1963, Isolation of chromosomes, Exp. Cell Res. (Suppl.) 9:220–234.
Southern, E., 1975, Detection of specific sequences among DNA fragments separated by gel electrophoresis, J. Mol. Biol. 98:503–517.
Stubblefield, E., and Oro, J., 1982, The isolation of specific chicken macrochromosomes by zonal centrifugation and flow sorting. Cytometry 2:273–281.
Stubblefield, E., and Wray, W., 1971, Architecture of the Chinese hamster metaphase chromosome, Chromosoma 32:262–294.
Stubblefield, E., and Wray, W., 1973, Biochemical and morphological studies of partially purified Chinese hamster chromosomes, Cold Spring Harbor Symp. Quant. Biol. 38:835–843.
Stubblefield, E., Cram, S., and Deaven, L., 1975, Flow microfluorometric analysis of isolated Chinese hamster chromosomes, Exp. Cell Res. 94:464–468.
Stubblefield, E., Linde, S., Franolich, F. K., and Lee, L. Y., 1978, Analytical techniques for isolated metaphase chromosome fractions, in: Methods in Cell Biology, Volume XVII (D. M. Prescott, ed.) Academic Press, New York, pp. 101–113.
Van Dilla, M. A., Carrano, A. V., and Gray, J. W., 1975, Flow karyotyping: Current status and potential development, in: Automation of Cytogenetics (M. L. Mendelsohn, ed.), U.S. ERDA Publication, pp. 145-164.
Wray, V. P., and Wray, W., 1982, Proteins of the metaphase chromosome, in: Biochemistry and Biology of the Cell Nucleus II. Nonhistone Proteins. (L. S. Hnilica, ed.), CRC Press.
Wray, V. P., Elgin, S. C. R., and Wray, W., 1980, Proteins of metaphase chromosomes and interphase chromatin, Nucleic Acids Res. 8:4155–4163.
Wray, W., 1973, Isolation of metaphase chromosomes, mitotic apparatus, and nuclei, in: Methods in Cell Biology, Volume VI (D. M. Prescott, ed.), Academic Press, New York, pp. 283–306.
Wray, W., 1976, Isopycnic centrifugation of mammalian metaphase chromosomes in Metrizamide, FEBS Lett. 62:202–207.
Wray, W., and Stefos, K., 1976, Quinacrine bands in isolated chromosomes, Cytologia 41:729–732.
Wray, W., and Stubblefield, E., 1970, A new method for the rapid isolation of chromosomes, mitotic apparatus, or nuclei from mammalian fibroblasts at near neutral pH, Exp. Cell Res. 59:469–478.
Wray, W., Stubblefield, E., and Humphrey, R., 1972, Mammalian metaphrase chromosomes with high molecular weight DNA isolated at pH 10.5, Nature New Biol. 238:237–238.
Wray, W., Zimmer, W., Berglund, E., and Schwartz, R., 1981, Chromosomal localization of the skeletal muscle α actin gene in chicken, J. Cell Biol. 91:68a.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1982 Plenum Press, New York
About this chapter
Cite this chapter
Wray, W., Stubblefield, E. (1982). Techniques to Isolate Specific Human Metaphase Chromosomes. In: Shay, J.W. (eds) Techniques in Somatic Cell Genetics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4271-7_25
Download citation
DOI: https://doi.org/10.1007/978-1-4684-4271-7_25
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4273-1
Online ISBN: 978-1-4684-4271-7
eBook Packages: Springer Book Archive