Abstract
Somatic cell genetics is a field which encompasses a number of parasexual techniques that can be used to transfer genetic information from one mammalian cell to another. These techniques include somatic cell hybridization (Ruddle and Creagan, 1975), microcell-mediated chromosome transfer (Fournier and Ruddle, 1977a), chromosome-mediated gene transfer (McBride and Ozer, 1973), and DNA-mediated gene transfer (Wigler et al., 1978). The distinguishing features of each approach are (a) the form in which donor genetic material is presented to recipient cells, and (b) the fraction of the donor genome ultimately retained by the cells. Microcell-mediated chromosome transfer occupies a unique position in this hierarchy: although only a fraction of the donor genome is transferred to the recipients, the introduced genetic material is retained as intact chromosomes. Thus, karyo-typically simple clones can be constructed which contain only one or a few introduced chromosomes. Since the transferred genetic material can be identified using cytogenetic tests, microcell hybrids constitute powerful gene mapping tools.
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References
Carter, S. B., 1967, Effects of cytochalasins on mammalian cells, Nature 213:261–264.
Davidson, R. L., and Gerald, P. S., 1976, Improved techniques forthe induction of mammalian cell hybridizations by polyethylene glycol, Somat. Cell Genet. 2:165–176.
Dickerman, L. H., and Goldman, R. D., 1973, A rapid method for production of binucleate cells, Exp. Cell Res. 83:433–436.
Ege, T., and Ringertz, N. R., 1974, Preparation of microcells by enucleation of micronucleate cells, Exp. Cell Res. 87:378–382.
Ege, T., Ringertz, N. R, Hamberg, H., and Sidebottom, E., 1977, Preparation of microcells, in: Methods in Cell Biology, Vol. XV (D. M. Prescott, ed.), Academic Press, New York, pp. 339–358.
Fournier, R. E. K., 1981, A general high efficiency procedure for production of microcell hybrids, Proc. Natl. Acad. Sci. USA 78:6349–6353.
Fournier, R. E. K., and Frelinger, J. A., 1982, Mol. Cell. Biol., in press.
Fournier, R. E. K., and Pardee, A. B., 1975, Cell cycle studies of mononucleate and cytochalasin B-induced binucleate fibroblasts, Proc. Natl. Acad. Sci. USA 72:869–873.
Fournier, R. E. K., and Ruddle, F. H., 1977a, Microcell-mediated transfer of murine chromosomes into mouse, Chinese hamster, and human somatic cells, Proc. Natl. Acad. Sci. USA 74:319–323.
Fournier, R. E. K., and Ruddle, F. H., 1977b, Microcell-mediated chromosome transfer, in: Molecular Human Cytogenetics (R. S. Sparkes, D. E. Comings, and C. F. Fox, eds.), Academic Press, New York, pp. 189–199.
Fournier, R. E. K., and Ruddle, F. H., 1977c, Stable association of the human transgenome and host murine chromosomes demonstrated with trispecific microcell hybrids, Proc. Natl. Acad. Sci. USA 74:3937–3941.
Fournier, R. E. K., Juricek, D. K., and Ruddle, F. H., 1979, Somatic cell genetic analysis of transgenome integration, Somat. Cell Genet. 5:1061–1077.
Gopalakrishnan, T. V., and Thompson, E. B., 1975, A method for enucleating cultured mammalian cells, Exp. Cell Res. 96:435–439.
Kozak, C. A., Fournier, R. E. K., Leinwand, L. A., and Ruddle, F. H., 1979, Assignment of the gene governing cellular ouabain resistance to Mus musculus chromosome 3 using human/mouse microcell hybrids, Biochem. Genet. 17:23–34.
Leinwand, L., Fournier, R. E. K., Nichols, E. A., and Ruddle, F. H., 1978, Assignment of the gene for adenosine kinase to chromosome 14 in Mus musculus by somatic cell hybridization, Cytogenet. Cell Genet. 21:77–85.
Levan, A., 1954, Colchicine-induced C-mitosis in two mouse ascites tumors, Hereditas 40:1–64.
McBride, O. W., and Ozer, H. L., 1973, Transfer of genetic information by purified metaphase chromosomes, Proc. Natl. Acad. Sci. USA 70:1258–1262.
McNeill, C. A., and Brown, R. L., 1980, Genetic manipulation by means of microcell-mediated transfer of normal human chromosomes into recipient mouse cells, Proc. Natl. Acad. Sci. USA 77:5394–5398.
Mercer, W. E., and Schlegel, R. A., 1979, Phytohemagglutinin enhancement of cell fusion reduces polyethylene glycol cyotoxicity, Exp. Cell Res. 120:417–421.
O’Malley, K. A., and Davidson, R. L., 1977, A new dimension in suspension fusion techniques with polyethylene glycol, Somat. Cell Genet. 3:441–448.
Phillips, S. G., and Phillips, D. M., 1969, Sites of nucleolus production in cultured Chinese hamster cells, J. Cell Biol. 40:248–268.
Poste, G., and Reeve, P., 1972, Enucleation of mammalian cells by cytochalasin B. II. Formation of hybrid cells and heterokaryons by fusion of anucleate and nucleated cells, Exp. Cell Res. 73:287–294.
Prescott, D. M., Myerson, D., and Wallace, J., 1972, Enucleation of mammalian cells with cytochalasin B, Exp. Cell Res. 71:480–485.
Ruddle, F. H., and Creagan, R. P., 1975, Parasexual approaches to the genetics of man, Ann. Rev. Genet. 9:407–486.
Smiley, J. R. Steege, D. A., Juricek, K. D., Summers, W., and Ruddle, F. H., 1978, A Herpes Simplex Virus 1 integration site in the mouse genome defined by somatic cell genetic analysis, Cell 15:455–468.
Stubblefield, E., 1964, Cytogenetics of Cells in Culture, Volume 3 (R. J. C. Harris, ed.), Academic Press, New York, pp. 223–298.
Wigler, M. H., and Weinstein, I. B., 1975, A preparative method for obtaining enucleated mammalian cells, Bioch. Biophys. Res. Commun. 63:669–674.
Wigler, M., Pellicer, A., Silverstein, S., and Axel, R., 1978, Transfer of single copy eukaryotic genes using total cellular DNA as donor, Cell 14:725–731.
Worton, R., Duff, C., and Flintoff, W., 1981, Microcell-mediated cotransfer of genes specifying methotrexate resistance, emetine sensitivity, and chromate sensitivity with Chinese hamster chromosome 2, Mol. Cell Biol. 1:330–335.
Wright, W. E., and Hayflick, L., 1972, Formation of anucleate and multinucleate cells in normal and SV-40 transformed WI-38 by cytochalasin B, Exp. Cell Res. 74:187–194.
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© 1982 Plenum Press, New York
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Fournier, R.E.K. (1982). Microcell-Mediated Chromosome Transfer. In: Shay, J.W. (eds) Techniques in Somatic Cell Genetics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4271-7_23
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DOI: https://doi.org/10.1007/978-1-4684-4271-7_23
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