Abstract
The analysis of genetic mechanisms underlying phenotypic expression in somatic cells depends on techniques which can demonstrate the presence of particular heritable components. For genetic elements located in the nucleus of the cell, numerous chromosomal, drug resistance, and biochemical markers are now available which allow study of specific loci. Similarly, for the analysis of mitochondrial inheritance in somatic cells there has been increased availability of drug resistance markers which can be transmitted by cytoplasmic transfer (Bunn et al., 1974; Lictor and Getz, 1978; Harris, 1978). Specific differences in buoyant density and restriction endonuclease digest patterns of mitochondrial DNA also have served as markers for the identification of cytoplasmic genetic determinants (Dawid et al., 1974; Case and Wallace, 1981).
“Although the law, that the substances giving the definite and hereditary characters to the cell are entirely contained in the nucleus, is at times spoken of as a very probable hypothesis ... ”
“Simple reflection shows ... that the determination whether or not this Theory of Inheritance (Vererbungs-Theorie) is true, can be settled in one way alone, viz., to take two different sorts of cells, utilizing the nucleus of one and the protoplasm of the other, to form a new cell. If the nucleus and protoplasm are so constituted that they can exist together, then will the properties arising from this cell, made artificially, answer our question. For then either the exclusive qualities of that cell will develop which had held the nucleus, or those will arise that come from the protoplasm, or lastly, from a mixture of both . . . ” (Boveri, 1893).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Attardi, B., and Attardi, G., 1972, Fate of mitochondrial DNA in human-mouse somatic cell hybrids, Proc. Natl. Acad. Sci. USA 69:129–133.
Boveri, T., 1893, An organism produced sexually without characteristics of the mother, Am. Naturalist 27:222–232.
Bunn, C. L., Wallace, D. C., and Eisenstadt, J. M., 1974, Cytoplasmic inheritance of chloramphenicol resistance in mouse tissue culture cells, Proc. Natl. Acad. Sci. USA 71:1681–1685.
Case, J. T., and Wallace, D. C., 1981, Maternal inheritance of mitochondrial DNA polymorphisms in cultured human fibroblasts, Somat. Cell Genet. 7:103–108.
Clayton, D. A., Teplitz, R. L., Nabholz, M., Dovey, H., and Bodmer, W., 1971, Mitochondrial DNA of human-mouse cell hybrids, Nature 234:560–562.
Coon, H. G., Horak, I., and Dawid, I. B., 1973, Propagation of both parental mitochondrial DNAs in rat-human and mouse-human hybrid cells, J. Mol. Biol. 81:285–298.
Dawid, I. G., Horak, I., and Coon, H. G., 1974, The use of somatic cells as an approach to mitochondrial genetics in animals, Genetics 78:459–479.
De Francesco, L., Attardi, G., and Croce, C. M., 1980, Uniparental propagation of mitochondrial DNA in mouse-human cell hybrids, Proc. Natl. Acad. Sci. USA 77:4079–4083.
Ditta, G., Soderberg, K., Landy, F., and Scheffler, I. E., 1976, The selection of Chinese hamster cells deficient in oxidative energy metabolism, Somat. Cell Genet. 2:331–344.
Doersen, C., and Stanbridge, E. J., 1979, Cytoplasmic inheritance of erythromycin resistance in human cells, Proc. Natl. Acad. Sci. USA 76:4549–4553.
Dujon, B., Kruszewska, A., Slonimski, P. P., Bolotin-Fukuhara, M., Coen, D., Deutsch, J., Netter, P., and Weill, L., 1975, Mitochondrial Genetics X: Effects of UV irradiation on transmission and recombination of mitochondrial genes in Saccharomyces cerevisiae, Mol. Gen. Genet. 137:29–72.
Eliceiri, G. L., 1973, The mitochondrial DNA of hamster-mouse hybrid cells, FEBS Lett. 36:232–234.
Gear, A. R. L., 1974, Rhodamine 6G: A potent inhibitor of mitochondrial oxidative phosphorylation, J. Biol. Chem. 249:3628–3637.
Gillham, N. W., Boynton, J. E., and Lee, R. W., 1974, Segregation and recombination of non-Mendelian genes in Chlamydomonas, Genetics 78:439–457.
Graves, J. A. M., 1972, Cell cycles and chromosome replication patterns in interspecific somatic hybrids, Exp. Cell Res. 73:81–94.
Graves, J. A. M., and Koschel, K. W., 1980, Changes in the cell cycle during culture of mousechinese hamster cell hybrids, J. Cell. Physiol. 102:209–216.
Harris, M., 1978, Cytoplasmic transfer of resistance to antimycin A in Chinese hamster cells, Proc. Natl. Acad. Sci. USA 75:5604–5608.
Harris, M., 1980, Pyruvate blocks expression of sensitivity to antimycin A and chloramphenicol, Somat. Cell Genet. 6:699–708.
Humphrey, R. M., and Hsu, T. C., 1965, Further studies on biological properties of mammalian cell lines resistant to 5-bromodeoxyuridine, Texas Rep. Biol. Med. 23:321.
Jami, J., and Grandchamp, S., 1971, Karyological properties of human-mouse somatic hybrids, Proc. Natl. Acad. Sci. USA 68:3097–3101.
Johnson, L. V., Walsh, M. L., and Chen, L. B., 1980, Localization of mitochondria in living cells with rhodamine 123, Proc. Natl. Acad. Sci. USA 77:990–994.
Lanfranchi, G., and Marin, G., 1981, Evidence for the derivation of mammalian somatic hybrids from polykaryocytes, Exp. Cell Res. 133:255–260.
Lichtor, T., and Getz, G. S., 1978, Cytoplasmic inheritance of rutamycin resistance in mouse fibroblasts, Proc. Natl. Acad. Sci. USA 75:324–328.
Mascarello, J. T., Soderberg, K., and Scheffler, I. E., 1980, Assignment of a gene for succinate dehydrogenase to human chromosome 1 by somatic cell hybridization, Cytogenet. Cell Genet. 28:121–135.
Matsuya, H., and Green, H., 1969, Somatic cell hybrid between the established human line D98 (presumptive HeLa) and 3T3, Science 163:697–698.
Sager, R., and Ramanis, Z., 1967, Biparental inheritance of nonchromosomal genes induced by ultraviolet irradiation, Proc. Natl. Acad. Sci. USA 58:931–937.
Sager, R., and Ramanis, Z., 1973, The mechanism of maternal inheritance in Chlamydomonas: Biochemical and genetic studies, Theor. Appl. Genet. 43:101–108.
Strugger, S., 1938, Die Vitalfärbung des Protoplasmas mit Rhodamin B und 6G, Protoplasma 30:85–100.
Wallace, D. C., and Eisenstadt, J. M., 1979, Expression of cytoplasmically inherited genes for chloramphenicol resistance in interspecific somatic cell hybrids and cybrids, Somat. Cell Genet. 5:373–396.
Ziegler, M. L., and Davidson, R. L., 1979, The effect of hexose on chloramphenicol sensitivity and resistance in Chinese hamster cells, J. Cell. Physiol. 98:627–636.
Ziegler, M. L., and Davidson, R. L., 1981, Elimination of mitochondrial elements and improved viability in hybrid cells, Somat. Cell Genet. 7:73–88.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1982 Plenum Press, New York
About this chapter
Cite this chapter
Ziegler, M.L. (1982). Mitochondrial Influences in Hybrid Cells. In: Shay, J.W. (eds) Techniques in Somatic Cell Genetics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4271-7_15
Download citation
DOI: https://doi.org/10.1007/978-1-4684-4271-7_15
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4273-1
Online ISBN: 978-1-4684-4271-7
eBook Packages: Springer Book Archive