Abstract
During the past few years attempts have been made by different groups to develop methods for the introduction of macromolecules into living cells1-3. Such technology can serve as an excellent tool for the study of various problems in modern cell biology and medicine, which otherwise could not have been studied.
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References
G. Poste, and D. Papahadjopoulos, Lipid vesicles as carriers for introduction of biologically active materials, in: “Methods in Cell Biology,” Vol. 4, D.M. Prescott, ed., Academic Press, New York (1976).
R.G. Kulka and A. Loyter, The use of fusion methods for the microinjection of animal cells, in: “Current Topics in Membranes and Transport,” Vol. 12, F. Bronner and A. Kleinzeller, eds., Academic Press, New York (1979).
M. Furusawa, M. Yamaizumi, T. Nishimura, T. Uchida and Y. Okada, Use of erythrocyte ghosts for injection of substances into animal cells by cell fusion, in: “Methods in Cell Biology”, Vol. 14, D.M. Prescott, ed., Academic Press, New York (1976).
M. Wasserman, N. Zakai, A. Loyter and R.G. Kulka, A quantitative study of ultramicroinjection of macromolecules into animal cells, Cell, 7: 551 (1976).
A. Loyter, N. Zakai and R.G. Kulka, “Ultramicroinjection” of macromolecules or small particles into animal cells, J. Cell Biol., 66: 292 (1975).
M. Furusawa, T. Nishimura, M. Yamaizumi and Y. Okada, Injection of foreign substances into single cells by cell fusion, Nature, 249: 449 (1974).
A.B. Schlegel, and C.M. Rechsteiner, Microinjection of thymidine kinase and bovine serum albumin into mammalian cells by fusion with red blood cells, Cell, 5: 371 (1975).
R.E. Pagano and J.N. Weinstein, Interactions of liposomes with mammalian cells, Annu. Rev. Biophys. Bioeng., 7: 435 (1978).
T. Uchida, M. Yamaizumi, E. Mekada and Y. Okada, Methods using HVJ (Sendai virus) for introducing substances into viable cells, in: “Introduction of Macromolecules into Mammalian Cells,” Alan B. Liss, Inc. (1980).
D.J. Volsky and A. Loyter, An efficient method f.or reassembly of fusogenic Sendai virus envelopes after solubilization of intact virions with Triton X-100, FEBS Lett., 92: 190 (1978).
A. Loyter, and D.J. Volsky, The use of reconstituted Sendai virus envelopes as carriers for the introduction of biological material into animal cells, in: “Cell Surface Reviews,” G. Poste and G.L. Nicolson, eds., North Holland Publishing Co., Amsterdam (in press).
D.J. Volsky, Z.I. Cabantchik, M. Beigel and A. Loyter, Implantation of the isolated human erythrocyte anion channel into plasma membranes of Friend erythroleukemic cells by use of Sendai virus envelopes, Proc. Natl. Acad. Sci. USA, 76: 5440 (1979).
Z.I. Cabantchik, D.J. Volsky, H. Ginsburg and A. Loyter, Reconstitution of the erythrocyte anion transport system: In vitro and in vivo approaches, in: “Annals of the New York Academy of Science”, Vol. 341 (1980).
Y. Hosaka and K.Y. Shimizu, Artificial assembly of envelope particles of HVJ (Sendai virus). I. Assembly of hemolytic and fusion factors from envelopes solubilízed by Nonidet P-40, Virology 49: 627 (1972).
A. Loyter and D.J. Volsky, The use of reconstituted Sendai virus envelopes for microinjection of macromolecules or for transfering of membrane components into animal cells, in: “Membrane Bioenergetics,” C.P. Lee, G. Schatz and L. Ernster, eds., Addison-Wesley Publ. Co., Massachusetts (1979).
T. Uchida, M. Yamaizumi and Y. Okada, Reassembled HVJ (Sendai virus) envelopes containing non-toxic mutant proteins of diphtheria toxin show toxicity to mouse L-cells, Nature, 266: 839 (1977).
A. Vainstein, J. Atidia and A. Loyter, Reconstituted Sendai virus envelopes as a biological carrier for microinjection of proteins and DNA molecules into animal cells, in “Liposomes in the Study of Drug Activity and Immunocompentent Cell Function,” A. Paraf and C. Nicolau, eds., Academic Press, London, New York (1981).
A. Loyter, A. Vainstein, M. Graessmann and A. Graessmann, Fusion mediated injection of SV40-DNA: Introduction of SV40-DNA into tissue culture cells by the use of DNA loaded reconstituted Sendai virus envelopes, Proc. Natl. Acad. Sci. USA (submitted for publication).
Z.I. Cabantchik, A. Knauf and A. Rothstein, The anion transport system of the red blood cell: The role of membrane protein evaluated by the use of “probes”, Biochim. Biophys. Acta, 515: 239 (1978).
A. Loyter, D. Ginsberg, D.J. Volsky, N. Zakai and Y. Laster, Association between enveloped viruses and host cell membrane: A specific case of ligand receptor interaction, in: “Receptors for Neurotransmitters and Peptide Hormones,” G. Pepeu, M.J. Kuhar and S. Enna, eds., Raven Press, New York (1980).
S. Israel, N. Zakai, D. Ginsberg, Y. Laster, Z.I. Cabantchik, J. Gordon and A. Loyter, Proteolysis and its possible role in virus-cell fusion, in: “13th FEBS Meeting,” Jerusalem (1980) (Abstract).
G. Tarone, N. Hamasaki, M. Fukuda and U.T. Marchesi, Proteolytic degradation of human erythrocyte band 3 by membrane associated protease activity, J. Memb. Biol. 48: 1 (1979).
A.Prujansky-Jakobowitz, D.J. Volsky, A. Loyter and N. Sharon, Alteration of lymphocyte surface properties by insertion of foreign functional components of plasma membranes, Proc. Natl. Acad. Sci. USA, 77: 7247 (1980).
D.J. Volsky, A.L. Richter, T. Dalianis and G. Klein, Implantation of mouse histocompatibility antigensinto membranes of cultured tumoral cells, Eur. J. Immunol. (in press).
D.J. Volsky, M.I. Shapiro and G. Klein, Transfer of Epstein-Barr virus receptors to receptor negative cells permits virus penetration and antigen expression, Proc. Natl. Acad. Sci. USA, 77: 5453 (1980).
M.R. Steinman, E. Brodie and A.Z. Cohn, Membrane flow during pinocytosis: A sterologic analysis, J. Cell Biol., 68: 665 (1976).
Y. Okada, Factors in of cells by HVJ, in: “Current Topics in Microbiology and Immunology,” Vol. 48, W. Arber and A. Braun, eds., Springer-Verlag, Berlin (1969).
Y. Maeda, J. Kim, I. Koseki, E. Mekada, Y. Shiokawa and Y. Okada, Modification of cell membranes with viral envelopes during fusion of cells with HVJ (Sendai virus), Exp. Cell. Res., 108: 95 (1977).
A. Lalazar and A. Loyter, Involvement of spectrin in membrane fusion: Induction of fusion in human erythrocyte ghosts by proteolytic enzymes and its inhibition by antispectrin antibody, Proc. Natl. Acad. Sci. USA, 76: 318 (1979).
A. Helenius, J. Kartenbeck, K. Simons and E. Fries, On the entry of Semliki Forest virus into BHK-21 cells, J. Cell Biol. 84: 404 (1980).
J. White, J. Kartenbeck and A. Helenius, Fusion of Semliki Forest virus with the plasma membrane can be induced by low pH, J. Cell Biol., 87: 264 (1980).
M.C. Hsu, A. Scheid and W.P. Choppin, Activation of the Sendai virus fusion protein (F) involves a conformational change with exposure of a new hydrophobic region, J. Biol. Chem. 256: 3557 (1981).
G.D. Eytan, Liposome cell interaction: Adsorption of ferritin loaded liposomes. Introduction of ferritin in cytoplasm of Ehrlich ascites tumor cells, J. Cell Biol., (submitted for publication).
A.E. Gad, R. Broza and G.S. Eytan, Fusion of cells and proteo—liposomes: Incorporation of beef heart cytochrome oxidase into rabbit erythrocytes, FEBS Lett., 102: 230 (1979).
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Beigel, M., Eytan, G., Loyter, A. (1982). Reconstituted Sendai Virus Envelopes as a Vehicle for the Introduction of Soluble Macromolecules and Membrane Components into Animal Cells. In: Gregoriadis, G., Senior, J., Trouet, A. (eds) Targeting of Drugs. NATO Advanced Study Institutes Series, vol 47. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4241-0_8
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DOI: https://doi.org/10.1007/978-1-4684-4241-0_8
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