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Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins

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Experimental Neurochemistry

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Abstract

O’Farrell1 introduced the first high-resolution method for analyzing heterogenous populations of proteins. Earlier attempts had met with only limited success. O’Farrell’s1 two-dimensional Polyacrylamide gel electrophoresis (20-PAGE) technique separates proteins according to isoelectric point in one dimension and molecular weight in the second dimension. The independence of these separation criteria for proteins contributes to the high resolving power of the technique. After minor modifications, O’Farrell’s1 technique was first used to analyze nervous system proteins in my laboratory.2–5

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Author information

Authors and Affiliations

  1. Department of Physiology and Biophysics, University of Miami, School of Medicine, Miami, Florida, 33101, USA

    David L. Wilson

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  1. David L. Wilson
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Editors and Affiliations

  1. Center for Neurochemistry, Ward’s Island, New York, USA

    Abel Lajtha

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© 1982 Plenum Press, New York

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Wilson, D.L. (1982). Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins. In: Lajtha, A. (eds) Experimental Neurochemistry. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4208-3_6

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  • DOI: https://doi.org/10.1007/978-1-4684-4208-3_6

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-4210-6

  • Online ISBN: 978-1-4684-4208-3

  • eBook Packages: Springer Book Archive

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