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Rapid Enzyme Inactivation

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Experimental Neurochemistry

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Abstract

The brain functions at a high metabolic level and is dependent on the maintenance of energy stores that require constant access to oxygen and glucose via cerebral blood flow. The enzyme activities of individual metabolic pathways and relative concentrations of intermediary metabolites can be significantly altered by changes in regional blood flow in the brain. In order to determine endogenous metabolite levels, cessation of metabolic events should ideally occur in situ instantaneously, since any finite amount of time provides relative periods of ischemia during which artifactual changes in metabolite concentrations can occur. Efforts to measure in vivo levels of intermediary metabolites, high-energy phosphates, cyclic nucleotides, and amino acids in brain tissue require rapid enzyme inactivation to stop metabolism.

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Author information

Authors and Affiliations

  1. Neuroscience Research Unit, Department of Psychiatry, University of Vermont, Burlington, Vermont, 05405, USA

    Robert H. Lenox

  2. Department of Medical Neurosciences, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D.C., 20012, USA

    G. Jean Kant & James L. Meyerhoff

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  1. Robert H. Lenox
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  2. G. Jean Kant
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  3. James L. Meyerhoff
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Editors and Affiliations

  1. Center for Neurochemistry, Ward’s Island, New York, USA

    Abel Lajtha

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© 1982 Plenum Press, New York

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Lenox, R.H., Kant, G.J., Meyerhoff, J.L. (1982). Rapid Enzyme Inactivation. In: Lajtha, A. (eds) Experimental Neurochemistry. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4208-3_4

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  • DOI: https://doi.org/10.1007/978-1-4684-4208-3_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-4210-6

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