Abstract
Many cellular activities are controlled via signals from outside the cell. Examples of extracellular signals include hormones and neurotransmitters which act on target cells by way of receptors to induce specific responses. Cells unresponsive to a given signal are nontargets and generally possess few, if any, receptors for that signal. Receptors are macromolecules that may exist as free and soluble components of the cytoplasm, may reside within various intracellular compartments, or may exist primarily on the outer surface of plasma membranes. Receptors function via their capacity to recognize and bind specific signals, i.e., ligands. Implicit in receptor theory is the assumption that the occupied receptor, i.e., receptor-ligand complex, is responsible for the production of a biological response. Although the correlation of receptor occupancy with biological response is an important issue in receptor studies, the assessment of receptor-ligand interactions is the primary subject of this chapter. We have recently reviewed the receptor occupancy/response relationship for steroid1 and neurotransmitter2 systems.
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References
Clark, J. H., and Peck, E.J., Jr., 1979, Female Sex Steroids: Receptors and Function, Springer-Verlag, New York.
Peck, E. J., Jr., and Lester, B. R., 1980, The Cell Surface and Neuronal Function (C. W. Cotman, G. Poste, and G. L. Nicolson, eds.), North-Holland, Amsterdam, New York, pp. 405–453.
Michaelis, L., and Menten, M. L., 1913, Biochem. Z. 49:333–369.
Scatchard, G., 1949, Ann. N.Y. Acad. Sci. 51:660–672.
Lineweaver, H., and Burk, D., 1934, J. Am. Chem. Soc. 56:658–666.
Eisenthal, R., and Cornish-Bowdin, A., 1974, Biochem. J. 139:715–720.
Rosenthal, H., 1967, Anal. Biochem. 20:525–532.
Munson, P. J., and Rodbard, D., 1980, Anal. Biochem. 107:220–239.
Feldman, H. A., 1972, Anal. Biochem. 48:317–338.
Lester, B. R., Miller, A. L., and Peck, E. J., Jr., 1981, J. Neurochem. 36:154–164.
Hollenberg, M. D., and Cuatrecasas, P., 1976, Methods in Cancer Research, Volume XII (H. Busch, ed.), Academic Press, New York, pp. 317–366.
Rodbard, D., and Feldman, H. A., 1975, Methods Enzymol. 36:3–6.
O’Brien, R. D. (ed.), 1979, The Receptors, Volume 1, Plenum Press, New York.
Birnbaumer, L., and O’Malley, B. W. (eds.), 1978, Receptors and Hormone Action, Volumes II, III, Academic Press, New York.
Keiner, K. L., Miller, A. L., and Peck, E. J., Jr., 1980, J. Recept. Res. 1:215–237.
Steggles, A. W., and King, R. J. B., 1970, Biochem. J. 118:695–701.
Korenman, S. G., Perrin, L. E., and McCallum, T. P., 1969, J. Clin. Endocrinol. 29:879–883.
Mester, J., Robertson, D. M., Feherty, P., and Kellie, A. E., 1970, Biochem. J. 120:831–836.
Sanborn, B. M., Rao, B. R., and Korenman, S. G., 1971, Biochemistry 10:4955–4961.
Clark, J. H., and Gorski, J., 1969, Biochim. Biophys. Acta 192:508–515.
Erdos, T., Best-Belpomme, M., and Bessada, R., 1970, Anal. Biochem. 37:244–252.
Santi, D. V., Sibley, C. H., Perriard, E. R., Tomkins, G., and Baxter, J. D., 1973, Biochemistry 12:2412–2416.
Peck, E. J., Jr., and Clark, J. H., 1977, Endocrinology 101:1034–1043.
Anderson, J. N., Clark, J. H., and Peck, E. J., Jr., 1972, Biochem. J. 126:561–567.
Zava, D. T., Harrington, N. Y., and McGuire, W. L., 1976, Biochemistry 15:4292–4297.
Roy, E. J., and McEwen, B. S., 1977, Steroids 30:657–669.
Hsueh, A. J., Peck, E. J., Jr., and Clark, J. H., 1974, Steroids 24:599–611.
Lester, B. R., and Peck, E. J., Jr., 1979, Brain Res. 161:79–97.
Cotman, C. W., and Mathews, D. A., 1971, Biochim. Biophys. Acta 249:380–394.
Jones, D. H., and Matus, A. I., 1974, Biochim. Biophys. Acta 356:276–287.
Cotman, C. W., Banker, G., Churchill, L., and Taylor, D., 1974, J. Cell Biol. 63:441–455.
Cohen, R. S., Blomberg, F., Berzins, K., and Siekevitz, P., 1977, J. Cell Biol. 74:181–203.
Cuatrecasas, P., 1971, Proc. Natl. Acad. Sci. U.S.A. 68:1264–1268.
Goldfine, I. D., Roth, J., and Birnbaumer, L., 1972, J. Biol. Chem. 247:1211–1218.
Posner, B. I., Kelly, P. A., Shiu, R. P. C., and Friesen, H. G., 1974, Endocrinology 95:521–531.
Brown, E. M., Aurbach, G. D., Hauser, D., and Troxier, F., 1976, J. Biol. Chem. 251:1232–1238.
Birnbaumer, L., Pohl, S. L., and Kauman, A. J., 1974, Adv. Cyclic Nucleotide Res. 4:239–281.
Rodbell, M., Krans, H. M. J., Pohl, S. L., and Birnbaumer, L., 1971, J. Biol. Chem. 246:1861–1871.
Zukin, S. R., Young, A. B., and Synder, S. H., 1974, Proc. Natl. Acad. Sci. U.S.A. 71:4802–4807.
Enna, S. J., and Snyder, S. H., 1975, Brain Res. 100:81–97.
Beaumont, K., Chilton, W. S., Yamamura, H. I., and Enna, S. J., 1978, Brain Res. 148:153–162.
Cuatrecasas, P., 1972, Proc. Natl. Acad. Sci. U.S.A. 69:318–322.
Iyengar, R., Abramowitz, J., Bordelon-Riser, M., Blume, A. J., and Birnbaumer, L., 1980, J. Biol. Chem. 255:10312–10321.
Hollenberg, M. D., and Cuatrecasas, P., 1979, The Receptors (R. D. O’Brien, ed.), Volume 1, Plenum Press, New York, pp. 193–214.
Munck, A., 1976, Receptors and Mechanism of Action of Steroid Hormones (J. R. Pasqualini, ed.), Volume 1, Marcel Dekker, New York, pp. 1–40.
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© 1982 Plenum Press, New York
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Peck, E.J., Kelner, K.L. (1982). Receptor Measurement. In: Lajtha, A. (eds) Experimental Neurochemistry. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4208-3_3
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DOI: https://doi.org/10.1007/978-1-4684-4208-3_3
Publisher Name: Springer, Boston, MA
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