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Selected Micromethods for Use in Neurochemistry

  • Chapter
Experimental Neurochemistry

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Abstract

Micromethods do not seem to be widely applied in neurochemical analysis, perhaps because of a widespread prejudice that micromethods are only useful for “microminded,” technically perfect experimenters. In addition, there may be worries about the reproducibility of microtechniques. A microgel, however, with many clearly separated and well-defined bands, each representing some nanograms of protein (see Fig. 10), is rather impressive when seen for the first time, especially when it is realized that the time taken for separation, staining, and destaining is only about one-tenth of the time necessary for the equivalent procedure using a macroscale method. A saving of experimental time is inherent in most of the micromethods described in some detail in this chapter. Such micromethods are also reproducible if they are performed correctly. The use of micromethods is therefore to be recommended even when the amount of material to be analyzed is not limited. Furthermore, now that it is harder to get sufficient funds for relevant research, micromethods are useful because the equipment needed for the analyses is not expensive.

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  1. Forschungsstelle Neurochemie, Max-Planck-Institut für Experimentelle Medizin, 3400, Göttingen, Federal Republic of Germany

    Volker Neuhoff

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  1. Center for Neurochemistry, Ward’s Island, New York, USA

    Abel Lajtha

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Neuhoff, V. (1982). Selected Micromethods for Use in Neurochemistry. In: Lajtha, A. (eds) Experimental Neurochemistry. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4208-3_16

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